Solmaz Kazemalilou; Nosratollah Najafi; adel reyhanitabar
Abstract
Introduction: Sunflower as one of the most important oil crops in the world is affected by water deficit stress. Water deficit stress is one of the most important limiting factors of crops growth and production in Iran and many regions of the world. Because of the low water productivity in Iran, the ...
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Introduction: Sunflower as one of the most important oil crops in the world is affected by water deficit stress. Water deficit stress is one of the most important limiting factors of crops growth and production in Iran and many regions of the world. Because of the low water productivity in Iran, the water conservation and increasing of water use efficiency through good management including integrated application of organic and chemical fertilizers and balanced nutrition of plants are necessary. One of the negative effects of water deficit stress is impaired uptake of nutrients such as phosphorus (P) by plants and a decrease in plant yield. It has been found that use of P can reduce the negative effects of drought stress on plants that means the tolerance of plants to drought stress increases with optimal nutrition of P. In Iran, due to the lack of organic matter and the high cost of chemical fertilizers, farmers tend to use organic wastes such as sewage sludge that can have an important contribution in the improvement of soil fertility and plant nutrition and lead to an increase in water use efficiency. Also, integrated and suitable nutrition of plants is a method of sustainable management of soil fertility under environmental stresses. Therefore, this study aimed to evaluate the effects of sewage sludge (SS) and triple superphosphate (TSP) on sunflower (Helianthus annuus L. cv. Farrokh) seed yield and its components and determining the optimal levels of their consumption under optimum and limited irrigation conditions.
Materials and Methods: This experiment was conducted as a split-plot factorial arrangement in a randomized complete block design in Agricultural Research Station of Khoy under field conditions for two years. Experimental factors were irrigation time at two levels (irrigation after 60 and 150 mm evaporation from class A evaporation pan), triple superphosphate at three levels (0, 100 and 200 kg/ha), sewage sludge at four levels (0, 14.2, 28.4 and 56.7 t/ha) and year at two levels (2014 and 2015) with three replications. The 1000 seed weight of sunflower, weight and number of seeds per plant, the percentage of empty achenes, head diameter and seed yield were measured at the end of plant growth period. Statistical analysis of the data was performed using MSTATC software and means comparison was done by Duncan’s multiple range test at 5% probability level.
Results and Discussion: The combined analysis of variances showed that the effect of year was significant for weight of seeds per plant, head diameter and seed yield but it is not significant for 1000 seed weight, number of seeds per plant and percentage of empty achenes. Although water deficit stress significantly increased the percentage of empty achenes but 1000 seed weight, weight and number of seeds per plant, head diameter and seed yield significantly decreased compared to optimum irrigation conditions. The application of integrated application of 200 kg TSP and 56.7 tons SS per ha increased yield and yield components significantly compared to the control treatment. The interaction effects of TSP×SS×irrigation were significant for all studied characteristics. Under optimum irrigation condition, the highest 1000 seed weight (64 g), weight of seeds per plant (85 g), number of seeds per plant (1513), head diameter (22 cm) and seed yield (5576 kg/ha) were observed in intergrated treatment of 200 kg TSP+56.7 tons SS/ha. Under limited irrigation condition, the highest 1000 seed weight (57 g), seeds weight per plant (81 g), seeds number per plant (1494), head diameter (19.6 cm) and seed yield (5322 kg/ha) were obtained from 56.7 tons SS/ha treatment which showed no significant difference with 100 kg TSP+56.7 tons SS/ha treatment. Under optimum and limited irrigation conditions, the percentage of empty achenes were significantly decreased by integrated treatments of 200 kg TSP+56.7 tons SS/ha and 100 kg TSP+56.7 tons SS/ha (70 and 81 percent, respectively) compared to the control.
Conclusion: The results of this study indicated that integrated application of triple superphosphate and sewage sludge can mitigate negative effects of drought stress; therefore in order to reduce the use of chemical fertilizers, increasing sunflower seed yield and development of sustainable agriculture, integrated treatments of 200 kg TSP+56.7 tons SS/ha under optimum irrigation and 100 kg TSP+56.7 tons SS/ha under limited irrigation can be recommended at similar conditions (from the aspect of soil, plant, sewage sludge and climate).
MirHassan Rasouli-Sadaghiani; Habib Khodaverdiloo; Mohsen Barin; Solmaz Kazemalilou
Abstract
Introduction: Heavy metals (HMs) are serious threat for environment due to their dangerous effects. These metals as contaminants that can be accumulated in soil and after absorption by plants, finally will be found in food chains. Cadmium (Cd) is one of the dangerous HMs that threats the health of plants, ...
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Introduction: Heavy metals (HMs) are serious threat for environment due to their dangerous effects. These metals as contaminants that can be accumulated in soil and after absorption by plants, finally will be found in food chains. Cadmium (Cd) is one of the dangerous HMs that threats the health of plants, living organisms and human. Physicochemical remediation methods may cause large changes in different characteristics of soils . Recently environmental-friendly strategies including phytoremediation have been emphasized by researchers. Phytoremediation that refers to the use of plants and their assistance with microorganisms for remediation of contaminated soils is an effective and low cost method for reclamation of heavy metals polluted soils. The most important limitation of phytoremediation is low availability of heavy metals and sensitivity of plants to contamination. There are evidences that soil microbes can help to overcome these limitations through several ways. Plant growth promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) are known to enhance plant growth and survival in heavy metal contaminated soils through different mechanisms including producing promoting metabolites, auxin, siderophore and antibiotics. In this study the role of some strains of PGPR (a mixture of Pseudomonas species including P. putida, P. fluorescens, and P. aeruginosa) and AMF (a mixture of Glomus species including G. intraradices, G. mosseae and G. fasciculatum), on uptake and accumulation of Cd, Fe, Zn and Cu as well as some physiological properties of Onopordon (Onopordon acanthium L) were evaluated.
Materials and Methods:This study was carried out under greenhouse condition as a factorial experiment based on a randomized complete block design with two factors including Cd concentration (four levels) and microbial treatment (three levels) in three replications. Consequently, a soil was selected and spiked uniformly with different concentrations of Cd (0, 10, 30 and 100 mg Cd kg-1 soil) at greenhouse of agricultural college in Urmia University. The contaminated soils were then sterilized and subsequently inoculated with arbuscular mycorrhizal fungi (a mixture of Glomose species including G. intraradices, G. mosseae and G. fasciculatum) and plant growth promoting rhizobacteria (a mixture of Pseudomonas species includeing P. putida, P. fluorescens, and P. aeruginosa). The seeds of Onopordon plants were grown in 2.5 kilogram pots under greenhouse condition. At the end of growing season the shoot dry weight, Cd, Fe, Zn and Cu concentration and element contents and some of physiological parameters of plant as well as microbial properties were analyzed. Furthermore, the effect of soil Pb level on population, activity and efficiency of the inoculated microbes was studied.
Results and Discussion: Significant difference was observed for plants’ dry weights. At different Cd levels, the yield of inoculated plants was higher than that of control plants. Furthermore, at elevated Cd concentration, plant height, biomass, relative yield, chlorophyll a, b, carotenoids, relative water content (RWC) decreased significantly (P < 0.05), however, plants inoculated with plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi showed considerable amount of dry matter, chlorophyll a, b as well as RWC. Mycorrizal and bacterial inoculation and Cd treatment also had significant effect on leaf photosynthetic pigments concentration and plant relative water content. In general, concentrations of photosynthetic pigments and RWC were higher in inoculated plants at every level of soil Cd. The microbial inoculation effectively decreased the inhibitory effects of Cd on plant growth. Shoot yield of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria plants increased by 2.7 and 2.1 times as well as microbial respiration increased by 2.17 and 2.01 times compared to control treatment. The results showed inoculated plant absorbed more Cd than non-inoculated plants. Plant growth promoting rhizobacteria were more effective than arbuscular mycorrhizal fungi inoculation in shoot Cd concentration. Cd contamination reduced soil microbial population and basal respiration. Results showed that with increasing soil Cd concentration shoot Fe, Zn and Cu concentrations significantly decreased. Root colonization rates decreased significantly with 10 mg kg-1 Cd addition for AMF treatments, and drastically with 100 mg kg-1 Cd added. Plant roots in the control and PGPR treatment were not colonized.
Conclusion: It is concluded that plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi inoculation could be sustained and promoted plant growth in phytoremediation processes. Therefore, under Cd contamination it can be use PGPR and AMF as growth promoters and finally enhance phytoremediation efficiency.
