J. Khallizadeh; E. Dordipour; M. Baranimotlgh; A. Gharanjiki
Abstract
Introduction: Iron deficiency is one of the most important nutritional disorders in plants, particularly in calcareous soils and deeply affects the yield and quality of the product. Due to the major role of iron in the synthesis of chlorophyll, chlorosis occurs in young leaves in deficiency conditions. ...
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Introduction: Iron deficiency is one of the most important nutritional disorders in plants, particularly in calcareous soils and deeply affects the yield and quality of the product. Due to the major role of iron in the synthesis of chlorophyll, chlorosis occurs in young leaves in deficiency conditions. In such condition, biochar can help to increase OM, soil fertility level, and iron use efficiency and, to reduce iron chlorosis. The aim of this study was to investigate the effect of iron- impregnated biochar on the availability of iron and the elimination of soybean iron chlorosis in a calcareous soil.
Materials and Methods: Calcareous soil with iron deficiency (0-30 cm) was collected from the east of Golestan province and prepared for cultivation. Two types of biochar were produced from wheat straw and particleboard through slow pyrolysis (increasing 5 °C/min) at 300 °C for 2 hours under restricted oxygen conditions in an electric furnace, and then impregnated with iron sulfate solution. FTIR spectra and SEM images of biochars surfaces were also provided. A pot experiment was conducted as a factorial based on a completely randomized design with four replications. Factors were biochars (wheat straw biochar (WB) and particleboard biochars (PB) each one with 2.5% w/w), iron impregnated biochars (Fe impregnated wheat straw biochar 2.5% w/w (Fe- IWB1) and 5% w/w (Fe-IWB2), 2.5% w/w (Fe-IPB1) and 5% w/w (Fe-IPB2) Fe impregnated particleboards, Fe- Sequestrene (S) and control without Fe and biochar (C), and two soybean cultivars (Williams and Saman). The sown pots were maintained near the field capacity for 12 weeks. Then, SPAD numbers, concentration and uptake of active iron in young and senile leaves and active iron content in soil were determined after harvest.
Results and Discussion: With increasing application of iron impregnated biochar, active iron content increased in the soil. SPAD numbers of the upper leaves of both soybean cultivars in Fe impregnated biochars were significantly higher than those of non-impregnated biochars and control treatments (P ≤ 0.05). Iron chlorosis symptoms in soybeans decreased following the increased application of Fe impregnated biochars, consequently, there were no iron chlorosis symptoms in 5% Fe impregnated biochar treatments. Also, the active iron concentration of the upper leaves and the amount of leaf active iron uptake significantly increased as a result of Fe impregnated biochars application in both soybean cultivars compared to control and non-impregnated biochars (P ≤ 0.05). The highest concentration of active iron in upper leaves was observed in 5% w/w Fe impregnated biochars treatments, but its value for cultivar Williams in Fe impregnated wheat biochar was higher than that in Fe impregnated particleboard biochar. The results of the SEM images indicated that wheat biochar had more quantity of and fine pores (also CEC) than that of the particleboard biochar, and the surface areas of both biochars were rough and dark after impregnation with iron, indicating the adsorption or accumulation of iron at their surfaces. Also, there was a significant positive correlation between the active iron concentration with SPAD numbers in the upper leaves (r = 0.88 **) and dry weight of soybean shoots (r = 0.87 **). Cultivars responses to Fe impregnated biochars showed that iron uptake and active iron concentration in the upper leaves of Williams variety were significantly less than those of Saman variety at both levels of Fe impregnated biochars (P ≤ 0.05), which indicates that cultivar Williams is more susceptible to the iron chlorosis. The results of this experiment and reports from other studies show that the application of impregnated biochars from nutrients besides increasing SOM, permeability and soil moisture, CEC and soil fertility level, also increases the acquisition and use efficiency of iron in the plant.
Conclusion: The results of this study showed that due to the strong adsorption of soil iron, non-impregnated biochar application in the level of 2.5% had no significant effect on the concentration and uptake of active iron and spad numbers of the plant. However, using Fe impregnated biochar and increasing their application in calcareous soils with iron chlorosis resulted in a significant increase of active soil iron content, concentration and uptake of active iron and SPAD numbers of the plant, and, conversely, a decrease of leaf chlorosis. Therefore, besides improving the physical, chemical and biological properties of the soil, the application of Fe impregnated biochar can also be a promising approach to eliminate iron chlorosis in sensitive plants, particularly soybeans in calcareous soils.
leila tabande; M. R.Bakhshi
Abstract
Introduction
Among essential plant microelements, iron (Fe) exert the highest restriction of crop production in Fars Province. Trace elements in the soil is composed of forms into 5 groups. These are the water-soluble and variable, adsorbed, chelate creating with complex compounds, secondary clay minerals, ...
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Introduction
Among essential plant microelements, iron (Fe) exert the highest restriction of crop production in Fars Province. Trace elements in the soil is composed of forms into 5 groups. These are the water-soluble and variable, adsorbed, chelate creating with complex compounds, secondary clay minerals, forms insoluble metal oxide minerals, and primary minerals. Water-soluble, changeable, adsorbed or form in chelates to be present as balance in the soil is noted and to be important for plants in nutrition.
Materials and Methods
In a greenhouse experiment was done with Soybean planting, the effects of Fe chelate (FeEDDHA) fertilizer levels on William cultivar of soybean (Glycine max L.) growth and chemical composition were studied by using a completely randomized design with 3 replications. Treatments were consisted of 10 soil samples and 3 levels of Fe applications (control, 5 and 10 mg.kg-1 as Fe EDDHA). Beside some physical and chemical soil properties and Extractable iron content with DTPA and EDTA were determined. By sequential extraction methods of Singh & Sposito ( 1982), chemical forms of Iron, exchangeable iron (Exch-Fe), organic bounded iron (OM-Fe), amorph iron oxides bounded iron (AFeOX-Fe), crystal iron oxides bounded iron (CFeOX-Fe) contents of soils were determined. Then, Concentration and uptake of Fe, Mn, Cu and Zn in plant were calculated.
Results and Discussion
carbonate, organically, amorphous oxide, crystalline iron oxide bounded and residual forms of iron were 0.0053, 0.0016, 0.44, 21.1 and 78.6% of the total iron as average, respectively. Therefore, content of carbonate, organically bound iron of soil, represented only a small fraction would not be considered as important as the total iron. In other words, crystalline iron oxide bound iron and residue iron forms constitued an important part of total iron.
Considering the average iron content of the soil related to chemical forms of iron was arrenged such as:
Res-Fe>CFeOX-Fe>AFeOX-Fe>Car-Fe> OM-Fe > Exch.-Fe
Applications of Fe had significant effect on dry matter, concentration, and uptake of Fe, Zn, Cu and Mn, extractable forms via extracting DTPA, EDTA, organic and exchangeable forms in soybean compared to control. Among chemical forms of iron, organic form with the amount of available iron plant (extraction by DTPA) had significant positive correlation. Also, many of the physical and chemical properties of calcareous soils studied, were significantly correlated with some chemical forms and amount of iron uptake by plant. DTPA extractable iron had negative correlation with pH ( R2= 0.514*) and EDTA extractable iron had positive correlation with organic matter (R2= 0.428*).
Conclusions
Application of Fe EDDHA, was leaded to significant increase organic and plant available (DTPA) forms of iron and due to significant regression equation (r=0.435*) between two chemical forms of iron (organic and DTPA extracted), it can be inferred that, the bulk of available iron plant was in form of organically bound. One reason for the positive reaction to the use of Fe EDDHA, subjected to a significant increase absorbable forms of iron in the studied soils.
Keywords: Chemical and Physical properties of soil, DTPA, EDTA, Iron, Sequential extraction
vahid mozafari; fariba khaleghi
Abstract
Introduction: Salinity is one of the main problems which limits crop production, especially in arid and semi-arid areas such as Iran. Iran is the most important producer of pistachio in the world. However, its performance is low in many areas. Most pistachio plantations are irrigated with saline water ...
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Introduction: Salinity is one of the main problems which limits crop production, especially in arid and semi-arid areas such as Iran. Iran is the most important producer of pistachio in the world. However, its performance is low in many areas. Most pistachio plantations are irrigated with saline water and with low quality (28). On the other hand, nitrogen is a dynamic element which is a constituent of amino acids, proteins, nucleic acids and Enzymes and it has a vital role in plant physiology, growth, chlorophyll formation and production of fruit and seeds (34). Gibberellic acid is known as phytohormon which varied physiological responses in plants under stress. acid gibberellic increases the photosynthesis and growth under stress and impact on the physiology and metabolism of plant (29). Based on previous studies, production and activity of plant hormones are affected by natural factors and plant nutrient requirements and the nitrogen has an important influence on production and transmission of acid gibberellic plant shoot. Therefore, in this study the effect of acid gibberellic and nitrogen on some characteristics of physiology parameters and micronutrient pistachio seedlings (Cv. Qazvini) under saline conditions was studied.
Materials and methods: Experiment under greenhouse condition and factorial in a completely randomized design with three replications was conducted in greenhouse agriculture college, Vali-E-Asr University of Rafsanjan. Treatments consisted of three levels of salinity (0, 1000 and 2000 mg of sodium chloride per kg of soil), three levels of nitrogen (0, 75 and 150 mg per kg of ammonium nitrate source) and three acid gibberellic levels (0, 250 and 500 mg per liter). Adequate soil with little available salinity conditions was collected from the top 30-cm layer of a pistachio-culture region of Kerman province. After air drying and ground through passing a 2 mm sieve, some of the physical-chemical properties of this soil include pH (7/63), Tissue (Sandy loam), electrical conductivity (ECe) (1 dS m-1), Silt (23.1%), Clay (5.5%), Organic matter (0.5%), Olsen phosphorus (P) (5.35 mg kg-1), Ammonium acetate-extractable K (100 mg kg-1) were determined. Nitrogen treatments 3 weeks after planting, dissolved in irrigation water was added to pots. Salinity, after the establishment of the plant (5 weeks after planting), divided into two equal parts and one-week interval dissolved with irrigation water was added to the pot. as well acid gibberellic treatments, as spray after salt treatment was applied at three times and at intervals of one week.
Results and discussion: The results showed that the salinity content of carotenoid and Chlorophyll fluorescence parameters significantly reduced but with increasing acid gibberellic and nitrogen application, mentioned parameters were significantly increased, compared to controls. The ability of photosynthesis improved and increased productivity. Mozafari et al studied the pistachio, reported that with increasing salinity from zero to 150 and 300 mM NaCl, carotenoids decreased more than 16% and 22% compared to control respectively. Carotenoids play a most important role in light, protecting plants against stress condition. Salinity application increased leaf proline, but with application of 150 mg nitrogen and 500 mg per liter foliar application of acid gibberellics, this parameter increased by 55 and 26 percent, respectively. Also, combined use of these two treatments increased proline content by 79 percent compared to control. The researchers stated that the increasing gibberellin concentration caused leaf proline increased, so spraying 100 and 200 mg per liter gibberellin significantly increased leaf proline compared with the non-application of gibberellin. The results also showed with increasing salinity increased iron, manganese and zinc concentrations shoots and roots and decreased copper concentrations, but using 150 mg of nitrogen and acid gibberellic consumption concentrations of copper element increased. Hojjat nooghi and Mozafari (28) reported, the used salinity of 60 mM NaCl increased shoot Fe concentration, but by applying the same amount of salinity in the root iron concentration decreased compared with the control. Research has shown that the copper concentration in the leaves and shoot of corn planted in soil decreased with increasing salinity. Micronutrient absorption reduction such as copper in salt condition can result in greater absorption of nutrients such as sodium, magnesium and calcium. The researchers in the study reported that with increasing nitrogen in the form of nitrate and ammonium, zinc concentration in plant tissues increased along with increasing salinity and lower shoot dry weight, zinc concentration was increased in two wheat cultivars too.
Conclusion: The results of this experiment showed that under saline conditions, acid gibberellic and nitrogen applied alone or in combination improved physiology parameters and increased nutrient concentration of pistachio seedling.
