تأثیر کاربرد برخی اسیدهای آلی بر ویژگی‌های رشدی و غلظت عناصر غذایی ذرت علوفه‌ای

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه زنجان

2 زنجان

3 دانشگاه پیام نور

4 تربیت مدرس تهران

چکیده

کاربرد اسیدهای آلی و معدنی در کشاورزی در سال‌های اخیر رو به افزایش است اما به نظر می‌رسد تأثیر آنها بر رشد و عملکرد گیاهان در این زمینه به پژوهش بیشتری نیاز دارد. هدف از این پژوهش مطالعه تأثیر کاربرد اسیدهای آلی و معدنی بر رشد گیاه ذرت علوفه‌ای در یک خاک آهکی بود. این آزمایش در گلخانه علوفه‌ای در قالب طرح آماری کاملاً تصادفی در 9 تیمار و 3 تکرار انجام شد. تیمارهای 1 و 2 کاربرد اسید سیتریک به ترتیب با غلظت 5 و 10میلی‌مولار، تیمار 3 و 4: کاربرد اسید استیک به ترتیب با غلظت 5 و 10 میلی‌مولار، تیمار 5 و 6: کاربرد اسید اگزالیک به ترتیب با غلظت 5 و 10 میلی‌مولار، تیمار 7: کاربرد مخلوط سه اسید آلی هرکدام با غلظت 33/3 میلی‌مولار، تیمار 8: کاربرد اسید سولفوریک با غلظت 5 میلی‌مولار، تیمار 9: بدون کاربرد اسید (شاهد) انجام شد. اسیدها با آب آبیاری در سه مرحله در طول فصل رشد اضافه شدند. طبق نتایج تیمار اسید سیتریک در هر دو سطح غلظتی نسبت به تیمار شاهد منجر به افزایش در وزن تر و خشک بخش هوایی شد. بیشترین غلظت نیتروژن در بخش هوایی در اسیدهای سیتریک، اگزالیک و اسید سولفوریک دیده شد. بیشترین غلظت پتاسیم (ریشه) و منگنز (بخش هوایی و ریشه) در تیمار اسید سولفوریک، بیشترین غلظت آهن (بخش هوایی) در تیمار اسید سیتریک و بیشترین غلظت روی (بخش هوایی و ریشه) در تیمار مخلوط اسیدهای آلی دیده شد.

کلیدواژه‌ها


عنوان مقاله [English]

Application Effects of Organic Acids on Growth of Forage Corn and Concentration of Nutritional Elements in Shoots and Roots

نویسندگان [English]

  • Akbar Hassani 1
  • Maryam Etemadian 2
  • mehdi nourzadeh haddad 3
  • Mehrdad Hanifeie 4
1 University of Zanjan
2 University of Zanjan
3 Payame Noor University (PNU)
4 Tarbiat Modares University
چکیده [English]

Introduction: In calcareous soils of Iran, using fertilizers that reduce soil pH over long periods are prioritized. Reducing pH in calcareous soils increases the concentration of essential nutrients such as phosphorus, iron, zinc, copper and manganese in the soil solution. The use of organic and inorganic acids in calcareous soils may also have other advantages in addition to gradually decreasing the soil solution pH. The effect of organic and minerals acids on plant growth and uptake of essential nutrients has not been studied. The aim of this study was to evaluate the effect of organic acids like acetic, citric and oxalic acid and mineral acids like sulfuric on the growth of forage corn.
Materials and Methods: The experiment was based on randomized complete block design and carried out in pots in a greenhouse. A calcareous soil with electrical conductivity of 0.86 dS m-1 and organic matter of 4.3 g kg-1 was collected from research farm of University of Zanjan. Treatments were T1 & T2: citric acid with concentration of 5 and 10 mM (C5 & C10), T3 & T4: acetic acid at a concentration of 5 and 10 mM (A5 & A10), T5 & T6: oxalic acid at a concentration of 5 and 10 mM (O5 & O10), T7: mixture of citric, acetic and oxalic acid each at a concentration of 3.33 mM (mix):, T8: sulfuric acid at a concentration of 5 mM (S), and T9: control. Treatments were applied in three stages: immediately after sowing, four-leaf and eight-leaf stages. Irrigation of pots was done with water with EC value of 400 μS /cm. Considering the possible effect of acids on increasing the availability of phosphorus, potassium, iron, zinc, copper and manganese, fertilization was done only based on nitrogen demand and 0.55 g urea was added to each pot (equivalent to 200 kg ha-1) with irrigation water in three steps. The shoots of plant samples were harvested after 50 days and the roots were carefully removed from the soil. Some growth related characteristics such as stem height, fresh weight, dry weight, and moisture content of vegetable tissue were also measured. Concentration of nitrogen, potassium, phosphorous, iron, zinc, manganese and copper in roots and shoots was measured. Translocation factor (TF) indicating the transfer rate of the elements from root to shoot was obtained by dividing the concentration of the element in the shoot by that in the root.
Results and Discussion: The results showed the significant effects of the treatments on the growth factor (fresh weight, dry weight and plant height). The percentage of moisture content was the same in all treatments. Citric acid treatment (T2) significantly increased fresh weight of shoot (18.3 percent) and dry weight (20.9 percent) of the plant. Organic acids also increased the concentration of nitrogen in shoots and roots. The concentration of nitrogen in the shoots was roughly twice as compared with that in the plant root. As for the potassium treatments, except for A10 treatment (T4) (the lowest concentration), other treatments did not show a significant difference with control. The highest concentration of potassium in roots was observed in sulfuric acid treatment (T8). The highest translocation factor of potassium (3.34) was observed in O10 treatment (T6). The results indicated a positive effect of 5 mM citric acid, acetic acid, mix treatment and sulfuric acid on shoot phosphorus and the positive effect of acetic acid and mix treatment on the phosphorus root. Citric acid treatments (T1 and T2) were the most effective treatments in increasing the concentration of iron (289 mg kg-1) in shoots. For roots, C10 treatment (T2) and Mix treatment (T7) showed the highest iron concentration. The highest TF for iron was observed in A10 treatment (T4). Acetic acid treatments (both concentrations), and sulfuric acid were more effective than other treatments and significantly increased the manganese concentration of the shoots. Sulfuric acid also caused a significant increase in the manganese concentration of the root. Acetic acid treatment (T5) showed the highest amount of TF for manganese. The amount of zinc element in shoots and roots was significantly affected by the mix treatment (T7). There was no significant difference between all Cu treatments.
Conclusions: In general, application of citric acid in both concentrations is useful to increase the biological yield and product quantity in maize farms. These treatments increased fresh and dry weight of shoots and roots. Acetic acid seems to improve translocation of elements in plants. The use of other acids is likely to enhance concentration of nutritional elements in roots and shoots.

کلیدواژه‌ها [English]

  • Calcareous soil
  • Citric
  • corn
  • Organic and mineral acids
1- Akhtar M., Yaqub M., Naeem A., Ashraf M., and Hernandez V.E. 2016. Improving phosphorus uptake and wheat productivity by phosphoric acid application in alkaline calcareous soils. Journal of the Science of Food and Agriculture, 96:3701-3707.
2- Azizabadi E., Golchin A., and Delavar M.A. 2014. Effect of potassium and drought stress on growth indices and mineral content of safflower leaf. Journal of Science and Technology of Greenhouse Culture, 5: 65-80. (In Persian with English abstract).
3- Backes C.A., McLaren R.G., Rate A.W., and Shift R.S. 1995. Kinetic of cadmium and cobalt desorption from iron and manganese oxides. Soil Science Society of America Journal, 59: 778-785.
4- Broadley M.R., White P.J., Hammond J.P., Zelko I., and Lux A. 2007. Zinc in plants. New Phytologist, 173:677-702.
5- Broschat T. K. 2006. Effects of Phosphorous and Phosphoric Acids on Growth and Phosphorus Concentrations in Container-grown Tropical Ornamental Plants. Technology and Product Reports, 16 (1):105-108.
6- Cayton M. T., Royes E. D., and Neve H. V. 1985. Effect of zinc fertilization on the mineral nutrition of rice differing in tolerance to zinc deficiency. Plant Soil, 87: 319– 327.
7- Choudhary F. M., and Loneragan J.F. 1970. Effect of nitrogen, copper and zinc fertilizers on the copper and zinc nutrition of wheat plants. Australian Journal of Agricultural Research, 21: 865–879.
8- Entezari M., Shariatmadari H., Jalalian A., and Taban M. 2012. Zinc and manganese nutrition of acacia, elm and plane trees in Isfahan landscape through hole mulching. Iranian Journal of Soil and Water Research, 43: 175-182. (In Persian with English abstract)
9- Etemadian M., Hassani A., Nourzadeh Haddad M., and Hanifei M. 2017. Effect of organic and inorganic acids on the release of nutrients in calcareous soils. Journal of Water and Soil Conservation, 24(5):73-91. (In Persian with English abstract)
10- Ezawa T., Smith S.E., and Smith F.A. 2002. P metabolism and transport in AM fungi. Plant and Soil, 244:221-230.
11- Gee G.W., and Or D. 2002. Particle-size analysis. In: Dane J.H., Topp G.C. (Eds.), Methods of Soil Analysis, Part 4. Physical Methods. Soil Science Society of America Book Series.
12- Gerke J., Römer W., and Jungk A. 1994. The excretion of citric and malic acid by proteoid roots of Lupinus albus L.; effects on soil solution concentrations of phosphate, iron, and aluminum in the proteoid rhizosphere in samples of an oxisol and a luvisol. Journal of Plant Nutrition and Soil Science, 157(4):289-94.
13- Hashmi Z.U.H., Khan M.J., Akhtar M., Sarwar T., and Khan M.J. 2017. Enhancing phosphorus uptake and yield of wheat with phosphoric acid application in calcareous soil. Journal of the Science of Food and Agriculture, 97:1733-1739.
14- Jiang Y.D., He Z.L., and Yang X.E. 2007. Effect of pH, organic acids, and competitive cations on mercury desorption in soils. Chemosphere, 69:1662-1669.
15- Jones D.L., and Darrah P.R. 1994. Role of root derived organic-acid in the mobilization of nutrient from the rhizosphere. Plant and Soil, 166:247-257.
16- Kalra Y. 1997. Handbook of Reference Methods for Plant Analysis. CRC press, London.
17- Khademi Z., Jones D. L., Malakouti M. J., Asadi F., and Ardebili M. 2009. Organic acid mediated nutrient extraction efficiency in three calcareous soils. Australian Journal of Soil Research, 47:213-220.
18- Ma L.Q., Komar K.M., Tu C., Zhang W., Cai Y., and Kennelley E.D. 2001. A fern that hyperaccumulates arsenic. Nature, 409(6820):579-579
19- Korte N., Skopp J., Fuller W., Niebla E., and Alesii B. 1976. Trace element movement in soils: Influence of soil physical and chemical properties. Soil Science, 122:350-359.
20- Labrada F. P., Mendoza A. B., Aguilar L. A. V., and Torres V. R. 2016. Citric acid in the nutrient solution increases the mineral absorption in potted tomato grown in calcareous soil, Pakistan Journal of Botany, 48 (1):67-74.
21- Mahmoodabadi M., Yazdanpanah N., Sinobas L.R., Pazira E., and Neshat A. 2013. Reclamation of calcareous saline sodic soil with different amendments (I): Redistribution of soluble cations within the soil profile. Agricultural Water Management, 120:30-38. (In Persian with English abstract)
22- Mesquite M.E., and Vierira J.M. 1996. Zinc adsorption by a calcareous soil. Copper interaction. Ceoderma, 69: 137-146.
23- Moafpourian G. 1995. The effect of zinc sources and sulfuric acid on growth and zinc uptake of maize plants and the chemical forms in the soil. MS thesis, Shiraz University, Iran. (In Persian)
24- Motsara M.R., and Roy R.N. 2008. Guide to laboratory establishment for plant nutrient analysis (Vol. 19). Rome: Food and Agriculture Organization of the United Nations.
25- Najafi N., Parsazadeh M., Tabatabaei S.J., and Oustan S. 2010. Effects of nitrogen form and pH of nutrient solution on the uptake and concentrations of potassium, calcium, magnesium and sodium in root and shoot of spinach plant. Water and Soil Science, 20: 111-131. (In Persian with English abstract)
26- Osorio D., and Mix K. 2016. Effects of organic acids application on olsen-extractable P and eggplant (Solanum melongena) yield. International Journal of Plant & Soil Science, 10(35):1-12.
27- Pacheco M. M. M., Garcia A. F., Gonzalez E. V., and Villegas M. A. C. 2011. Effect of citric acid on the proteolytic activity of Zea mays L., Ciência e Agrotecnologia, 5 (35):908.915. (In Spanish with English abstract)
28- Paul E.A. 2007. Soil Microbiology and Biochemistry. Third edithion. Linacre House, Jordan Hill, Oxford.
29- Paech K., and Tracey M.V. 2013. Modern Methods of Plant Analysis. Springer Science & Business Media. Verlag Berlin Heidelberg.
30- Piry M., and Sepehr E. 2015. Effect of citric acid on characteristics of zinc sorption in calcareous soils. Iranian Journal of Soil and Water Research, 46: 781-790. (In Persian with English abstract)
31- Poozeshi R., Zabihi H.R., Ramazani Moghadam M.R., Rajabzadeh M., and Mokhtari A. 2011. Yield and yield components of grape (Vitis vinefera cv.peykani) as affected by foliar application of zinc, humic acid and acetic acid. Journal of Horticulture Science, 25: 351-360. (In Persian with English abstract)
32- Rashid A., Choudhary F.M., and Sharif M. 1976. Micronutrient availability to cereals from calcareous soils. III. Zinc absorption by rice and its inhibition by important ions in submerged soils. Plant and Soil, 45:613– 623.
33- Rasouli-Sadaghiani M., Dareghayedi B., Khodaverdiloo H., and Moradi N. 2015. Effect of organic acids on sorption and immobilization of Fe in acidic and calcareous soils. Journal of Soil Management and Sustainable Production, 5:215-228. (In Persian with English abstract)
34- Rengel Z., and Romheld V. 2000. Root exudation and Fe uptake and transport in wheat genotypes differing in tolerance to Zn deficiency. Plant and Soil, 222:25-34.
35- Romheld V., and Awad F. 2000. Signicance of root exudates in acquisition of heavy metal from a contaminated calcareous soil by graminaceous species. Journal of Plant Nutrition, 23:1857-1866.
36- Sadiq M., Hassan G., Mehdi S., Hussain N., and Jamil M. 2007. Amelioration of saline-sodic soils with tillage implements and sulfuric acid application. Pedosphere, 17:182-190.
37- Sarikhani M.R., Malboubi M.A., and Ebrahimi M. 2014. Phosphate solubilizing bacteria: Isolation of bacteria and phosphate solubilizing genes, mechanism and genetics of phosphate solubilization. Journal of Agricultural Biotechnology, 6:77-110. (In Persian with English abstract)
38- Shao J.F., Yamaji N., Shen R.F., and Ma J.F. 2017. The Key to Mn homeostasis in plants: regulation of Mn transporters. Trends in Plant Science, 22(3):215-224
39- Simpson R.J. 1986. Translocation and metabolism of nitrogen: whole plant aspects. In: Lambers H., Neeteson J.J., Stulen I. (eds) Fundamental, Ecological and Agricultural Aspects of Nitrogen Metabolism in Higher Plants. Developments in Plant and Soil Sciences, vol 19. Springer, Dordrecht.
40- Taheripur A., Kiani S., and Hosseinpur A. 2015. Effect of EDTA and citric acid on phytoextraction of copper and zinc from a naturally contaminated soil by maize (Zea mays L.) cultivars. Journal of Water and Soil, 29:1493-1505. (In Persian with English abstract)
41- Tomas G.W. 1996. Soil pH and soil acidity. In: Sparks D.L., Page A.L., Helmke P.A., Loeppert R.H. (Eds.), Methods of Soil Analysis, Part 3. Chemical Methods. Soil Science Society of America Book Series.
42- Wallace A., Romney E.M., and Clark R.B. 1980. Corn inbreds differing in efficiency to Zn. Journal of Plant Nutrition, 2: 225–229.
43- Yang C.H., and Crowley D.E. 2000. Rhizosphere microbial community structure in relation to root location and plant iron nutritional status. Applied and Environmental Microbiology, 66:345-351.
44- Zhang F.S., Treeby M., Romheld V., and Marschner H. 1991. Mobilization of iron by phytosiderophores as affected by other micronutrients. Plant and Soil, 130:173-178.
45- Zolfi Baveryani M., and Maftoon M. 2011. Effect of zinc, copper and their chemical forms on growth and chemical composition of rice in a calcareous soil. Journal of Water and Soil Science, 14: 111-120. (In Persian with English abstract)
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