The Effect of Bacillus Bacteria on Phosphorus Release from Oil Refinery Plant-solid Waste in a Calcareous Soil

Document Type : Research Article

Authors

• E. Hashempour
• M.B. Farhangi
• N. Ghorbanzadeh
• M. Fazeli Sangani

University of Guilan

https://doi.org/10.22067/jsw.v34i1.80353

Abstract

Introduction: Due to the increasing development of edible oil processing industries, large amounts of wastewater and solid wastes (SW) are inevitable in these industries. Organic wastes can be used as soil conditioners in agriculture due to the high content of organic matter and nutrient loads. Phosphorus solubilizing bacteria including Bacillus spp., Pseudomonads and Rhizobium spp. can release phosphorus from insoluble organic and mineral sources in soil. Most soils in the semi-arid regions, including southern parts of Guilan province, have low organic matter content and do not support plant cultivation due to the low fertility and instability of soils. Hence, industrial wastes can be applied as a suitable and low-cost source of organic materials and nutrients in these soils. As phosphorus is one of the most important essential nutrients in plant nutrition which is also present in oil refinery soild wastes and P solubilizing bacteria can release phosphorus from the organic phase of the wastes and make it available in the soil solution, this study aimed to investigate the available phosphorus (Pava) content of soil after simultaneous addition of olive refinery-solid wastes and P solubilizing Bacillus spp.
Materials and Methods: the solid waste obtained from Ganje Rudbar oil refinery plant (located in Rudbar, Guilan province) and a soil sample was collected from a surface layer (0-30 cm) of a pasture, located in Lowshan area (Guilan province). A native strain of Bacillus sp. was isolated from the sampled soil based on its P-solubilizing ability in Sperber medium. An indicator strain, Bacillus persicus was also included in the experiments. P-solubilizing ability of the indicator strain was also evaluated in Sperber medium. The experiment was conducted in a completely randomized design based on factorial arrangement and three replications. Factors included three levels of solid waste (0, 2 and 4%), three levels of inoculated bacteria (no bacteria, native Bacillus sp. and Bacillus persicus) and eleven sampling times (0, 2, 7, 14, 28, 42, 56, 86, 116, 146, and 176 days). Different levels of solid waste were added to the soil, inoculated with bacteria (106 cell/g), and incubated at laboratory condition (~25 ºC) for six months. The moisture content of the soil mixtures fixed around 0.7 FC and kept constant during the incubation period. Sampling was done at desired times. The pH, organic carbon (OC), soil Basal Respiration (BR), available phosphorus concentration (Pava), and phosphatase enzyme activity were measured in soil samples. Data analysis and means comparison were done by Duncans’ test using SAS software package.
Results and Discussion: The studied soil was loam in texture, and had slightly alkaline pH, moderate Pava, and low OC content. The studied solid waste contained considerable OC and total P load. The effect of solid waste (SW), bacteria, sampling time and their interactions were significant on most of the measured characteristics (p < 0.05). SW application decreased soil pH and mixtures inoculated with native Bacillus sp. had lower pH values compared to those inoculated with Bacillus persicus, probably due to the greater effect of Bacillus spp. on SW decomposition compared with B. persicus. The highest average BR was attained in mixtures contained 4% SW which was 1.24 and 1.73 times greater than that in mixtures contained 2 and 0% SW, respectively. While the effect of SW on soil BR was obvious, bacteria inoculation had different impact on soil organic material decomposition and the lowest BR was measured in soil (0% SW) inoculated with Bacillus persicus. OC content of mixtures increased with SW application. The highest OC level (3.21 g 100g-1) was obtained in uninoculated mixture contained 4% SW, which was significantly greater than OC levels in mixtures inoculated with bacteria (p < 0.05). The lowest OC level (3.21 g 100g-1) was observed in uninoculated soil (0% SW). SW application significantly increased Pava. The greatest Pava concentration (142.77 mg Kg-1) was attained in uninoculated mixture contained 4% SW which was not significantly different from Pava concentration in 4% SW-mixture inoculated with native Bacillus sp. (P > 0.05). In control treatments (0% SW), Bacillus persicus was efficient in P release from soil native organic carbon and/or phosphate minerals. However, among the soils contained 2% SW, those inoculated with native Bacillus sp. had the highest Pava concentration. The average Pava concentration in the 4% SW-mixtures was 136.33 mg Kg-1 which was 3.5 times greater than that in control treatment (0% SW). Although soil Pava was related to phosphatase enzyme, this enzyme activity was not affected by treatments. In the P-releasing trend, it was found that 4% SW-mixtures had the highest Pava concentration after 6 months of incubation, and bacteria inoculation made the P-release trend to be flatter compared to control.
Conclusion: The application of oil refinery plant-solid waste improved the basal respiration of the studied soil and increased available phosphorus concentration. The comparison of applied solid waste levels showed that the inoculation of soil with Bacillus bacteria had a positive effect on available phosphorus concentration only at 2% solid waste level.

Keywords

• Microbial respiration
• Organic carbon
• Phosphatase
• Phosphate Solubilizing Bacteria

References

1- Ajami M., Khormali F., Ayoubi Sh., and Amoozadeh Omrani R. 2006. Changes in soil quality attributes by conversion of land use on a loess hillslope in Golestan Province, Iran. 1th international soil meeting (ISM) on soil sustaining life on earth, Managing Soil and Technology, 501-504.

2- Alvarez M., Huygens D., Diaz L.M., Villanueva C.A., Heyser W., and Boeckx P. 2012. The spatial distribution of acid phosphatase activity in ectomycorrhizal tissue depend on soil fertility and morphotype, and relates to host plant phosphorus uptake. Plant, Cell and Environment 35: 126-135.

3- Anderson T.H., and Domsch K.H. 1993. The metabolic quotient from CO2 (qCO2) as a specific activity parameter to assess the effects of environmental conditions, such as pH, on the microbial biomass of forest soils. Soil Biology and Biochemistry 25: 393-395.

4- Bloemberg G.V., Lugtenberg B.J.J. 2001. Effect of seed bacterization with fluorescent Pseudomonas on growth promotion of Jute in Terai zone of west Bengal, India. International Journal of current Microbiology and Applid Scienes 4: 343-350.

5- Chakarborty U., Chakraborty B.N., and Basnet M. 2006. Plant growth promotion and induction of resistance in Camellia chninesis by Bacillus megatarium. Journal of Basic Microbiology 46(3): 186-195.

6- Choudhary D.K., and Johri B.N. 2009. Interactions of Bacillus spp. and plants-with special reference to induced systemic resistance (ISR). Microbiological Research 164: 493-513.

7- Environmental regulations for reuse and recycling of waste water, 1389. Bulten No 535, Deputy Director of Strategic Control, Ministry of Energy, Iran. (In Persian)

8- Gomez-Munoz B., Hatch D.J., Bol R., and Garcia-Ruiz R. 2012. The compost of olive mill pomace: from a waste to a resource – environmental benefits of its application in olive oil groves. Chapter 20.

9- Gregorich E.G. and Carter M.R., 2007. Soil Sampling and Methods of Analysis. CRC press.

10- Hammond J.P., Broadley M.R., and White P.J. 2004. Genetic responses to phosphorus deficiency. Annals of Botany 94: 323-332.

11- Henkin T.M. 2016. Classic spotlight: bacterial endospore resistance, structure, and genetics. Journal of Bacteriology 198(14): 1904.

12- Khan A., Jilani G., Akhtar M.S., Saqlan Naqv S.M., and Rasheed M. 2009. Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. Agricultural Biology Science 1(11): 48-58.

13- Khosro M. 2012. Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production, Resources and Environment 2(1): 80-85.

14- Kirsten S.H., Donald R.Z., Kelly K.M., and Julie D.J. 2011. Changes in forest soil organic matter pools after a decade of elevated CO2 and O3, Soil Biology and Biochemistry, 43(7): 1518-1527.

15- Kloepper J.W., Ryu C. M., and Zhang S. 2004. Induce systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94: 1259-1266.

16- Kourtev P.S., Ehrenfeld J.G., and Huang W.Z. 2002. Enzyme activities during litter decomposition of two exotic and two native plant species in hardwood forests of New Jersey. Soil Biology and Biochemistry 34: 1207-1218.

17- Leboffe M.J., and Pierce B.E. 2012. Microbiology: Laboratory Theory and Application," Morton Publishing Company.

18- Loveland P., and Webb J. 2003. Is there a critical level of organic matter in the agricultural soils of temperate regions: a Review. Soil and Tillage Research 70: 1-18.

19- Marhual N.P., Pradhan N., Mohanta N.C., Sukla L.B., and Mishra B.K. 2011. Dephosphrization of LD slag by phosphorus solubilising bacteria, International Biodeterioration and Biodegradation 65: 404-409.

20- Marin L., and Fernandez-Escobar R. 1997. Optimization of nitrogen fertilization in olive orchards. In: Val, J., Montanes, L., Monge, E., (Eds.), Proceedings of the Third International Symposium on Mineral Nutrition of Deciduous Fruit Trees, Zaragoza, Spain, 411-414.

21- Mekki A., Arous F., Aloui F., and Sayadi S. 2013. Disposal of agro-industrials wastes as soil amendments. American Journal of Environmental Science 9(6): 458-469.

22- Navaro A.F., Cegarra J., Roig A., and Garcia D. 1993. Relationship between organic matter and carbon contents of organic wastes. Bioresource Technology 44(33): 203-207.

23- Regni L., Nasini L., Ilarioni L., Brunori A., Massaccesi L., Agnelli A., and Proietri P. 2017. Long term amendment with fresh and composted solid olive mill waste on olive grove affects carbon sequestration by pruning, fruits, and soil. Front Plant Science 7: 2042.

24- Rodriguez H., and Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17: 319–339.

25- Roldan A., Salinas G.J.R., Alguacil M.M., Diaz E., and Caravaca F. 2005. Soil enzyme activities suggest advantages of conservation tillage practices in sorghum cultivation under subtropical conditions. Geoderma 129: 178–185.

26- Saadi I., Laor Y., Raviv M., and Medina S. 2007. Land spreading of olive mill wastewater: effects on soil microbial activity and potential phytotoxicity. Chemosphere 66(1): 75-83.

27- Sankaralingam S., Harinathan B., Shankar T., Prabhu D., and Peer M. 2014. Effect of phosphate solublizing bacteria on growth and development of Sesbania grandiflora and Moringa oleifera. Sciences Agriculture 3(2): 88-96.

28- Shahab S., and Ahmed N., and Khan N.S. 2009. Indole acetic acid production and enhanced plant growth promotion by indigenous PSBs. African Journal of Agricultural Research 4(11): 1312-1316.

29- Sharma K., Dak G., Agarwal A., Bhatnagar M., and Sharma R. 2007. Effect of phosphate solubilizing bacteria on the the germination of Cicer arienatum seeds and seedling growth. Journal of Herbal Medicine and Toxicology 1(1): 61-63l.

30- Sharma R.K., Arawal M., and Marshalla F.M. 2006. Heavy metal contamination in vegetables grown in wastewater irrigated areas of Varanasi, India. Bulletion of Environmental contamination and Toxicology 77: 312-318.

31- Sierra J., Fontaine S., and Desfontaines L. 2001. Factors controlling N mineralization, nitrification and nitrogen losses in an Oxisol amended with sewage sludge. Soil Research 39: 519-534.

32- Sturz A.V., and Christie B.R. 2003. Beneficial microbial alleloplathies in the root zone: the management of soil quality and plant disease with rhizobacteria. Soil and Tillage Research 72: 107-123.

33- Tabatabai M.A. 1982. Soil enzymes, Methods of Soil Analysis. Part 2. American Society of Agronomy, Madison, WI, USA, 539–579.

34- Vessey J.K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil 255: 571–586.

35- Violante A., and Pigna M. 2002. Competitive sorption of arsenate and phosphate on different clay minerals and soils. Soil Science Society of American Journal 66: 1788-1796.

36- Williams M.A., Myrold D.D., and Bottomley P.J. 2006. Carbon flow from 13C-labeled straw and root residues into the phospholipid fatty acids of a soil microbial community under field conditions. Soil Biology and Biochemistry 38: 759–768.