دوماه نامه

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه یاسوج

2 دانشگاه شهرکرد

چکیده

ویژگی‌های زیستی و شیمیایی محیط ریزوسفر از جمله فراهمی و اجزاء شیمیایی فلزات در آن با توده خاک متفاوت است. این پژوهش به منظور بررسی تغییر در قابلیت‌استفاده و اجزاء مس در ریزوسفر آفتابگردان (Helianthus annuus L.) در یک خاک شنی آلوده به مس تیمار‌شده با کلات-کننده‌ها (EDTA، اسید سیتریک و عصاره کود مرغی) در شرایط گلخانه‌ای انجام شد.EDTA و اسید سیتریک در سطوح غلظتی صفر، 5/0 و 1 ‌میلی-مول بر کیلوگرم خاک و عصاره کود مرغی در سطوح غلظتی صفر، 5/0 و 1 گرم بر کیلوگرم خاک استفاده شدند. تعداد سه بذر آفتابگردان در هر ریزوباکس کاشته شد. بعد از 10 هفته گیاهان برداشت شدند و خاک‌های ریزوسفری و توده جدا شدند. مس قابل‌استفاده با استفاده از 7 روش عصاره‌گیری شیمیایی و جزءبندی مس در خاک‌های ریزوسفری و توده اندازه‌گیری شد. ویژگی‌های خاک ریزوسفری با خاک توده متفاوت بود. مقدار مس عصاره‌گیری شده به روش‌های مختلف در خاک‌های ریزوسفری کمتر از خاک‌های توده بود. دامنه تغییرات مس عصاره‌گیری شده با عصاره‌گیرها از 023/0 تا 83/1 میلی‌گرم بر کیلوگرم بود. در خاک‌های ریزوسفری و توده تیمارشده با کلات‌کننده‌ها، بیشترین مقادیر مس به ترتیب در اجزاء باقیمانده، پیوند‌شده با ماده آلی، پیوند‌شده با اکسیدهای آهن و منگنز، پیوند‌شده با کربنات‌ها و تبادلی بود. میانگین اجزاء مس پیوند‌شده با کربنات‌ها، پیوند‌شده با اکسیدهای آهن و منگنز و جزء باقیمانده در شرایط ریزوسفری بیشتر از توده خاک بود. میانگین جزء پیوند‌شده با ماده آلی در شرایط ریزوسفری کمتر از توده خاک بود. دامنه تغییرات اجزاء مس از 81/2 تا 95/12 میلی‌گرم بر کیلوگرم بود.

کلیدواژه‌ها

عنوان مقاله [English]

Change in Availability and Fractions of Copper in the Rhizosphere of Sunflower in a Contaminated Soil Treated With Chelators

نویسندگان [English]

  • Mohamad Rahmanian 1
  • AliReza Hosseinpour 2
  • Ebrahim Adhami 1
  • Hamidreza Motaghian 2

1 Yasouj University

2 Shahrekord University

چکیده [English]

Introduction: Rhizosphere is commonly defined as the zone where root activity significantly influences the biological and chemical properties of the soil. Biological, physical and chemical characteristics of rhizosphere, especially metal availability and metal chemical forms are different than the bulk soil. Plant roots continuously release compounds such as sugars, amino acids, and carboxylic acids. Plant roots have the ability to transform metal fractions for easier uptake through root exudation in the rhizosphere. This study was conducted to investigate change in availability and fractions of Copper in the rhizosphere of sunflower (Helianthus annuus L.) in a sandy contaminated soil treated with chelators (EDTA, citric acid and poultry manure extract (PME)) in greenhouse condition.
Materials and Methods: In this study, EDTA and citric acid were used at concentrations of 0, 0.5 and 1 mmol kg-1 soil and PME was used at concentrations of0, 0.5 and 1 g kg-1 soil. Three seeds of sunflower were planted in the rhizobox. After 10 weeks, plants were harvested and rhizosphere and bulk soils were separated. Dissolved organic carbon (DOC), microbial biomass carbon (MBC), available Cu (by using 7 chemical procedures including DTPA-TEA,AB-DTPA, Mehlich1, Mehlich3, CaCl2 0.01 M, rhizosphere-based method and distilled water) and Cu-fractions were determined in the rhizosphere and bulk soils.
Results and Discussion: Rhizosphere soils properties were different with bulk soils. The results showed that the mean of DOC and MBC in the rhizosphere soils were higher than the bulk soils, but this difference was significant in some treatments. The mean value of pH in the rhizosphere soils was significantly (p

کلیدواژه‌ها [English]

  • Chelator
  • copper
  • Fractionation
  • Rhizosphere
  • Sunflower
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