اثر قارچ‌های مایکوریزا- آربسکولار و فسفر بر جذب آرسنیک از خاک‌های آلوده به آرسنیت و آرسنات به‌وسیله گیاه آفتابگردان

نوع مقاله : مقالات پژوهشی

نویسندگان

• سعید باقریفام
• امیر لکزیان
• امیر فتوت
• رضا خراسانی

دانشگاه فردوسی مشهد

https://doi.org/10.22067/jsw.v0i0.29795

چکیده

در سال‌های اخیر آلودگی خاک‌ها و آب به آرسنیک به دلیل کاربردهای صنعتی و فعالیت‌های معدن‌کاری منجر به نگرانی‌های جدی زیست محیطی شده است. روش‌های مختلفی برای مدیریت زیست محیطی و پالایش خاک‌های آلوده پیشنهاد شده که در بین این روش‌ها گیاه پالایی به عنوان گزینه‌ای موثر و سازگار با محیط زیست مطرح شده است. به منظور پی بردن به اثرات همزیستی مایکوریزایی و غلظت فسفر بر جذب آرسنیت و آرسنات بوسیله گیاه آفتابگردان، مطالعه‌ای گلخانه‌ای به صورت کاملا تصادفی در قالب فاکتوریل با سه سطح مایکوریزا (شاهد، Glomus intraradices وmosseae Glomus)، دو سطح فسفر (0 و 60 میلیگرم بر کیلوگرم) و دو گونه معدنی آرسنیک (50 میلیگرم بر کیلوگرم آرسنیت یا آرسنات) انجام شد. نتایج حاصل از آزمایش نشان داد همزیستی مایکوریزایی غلظت آرسنیک در بخش هوایی گیاه را کاهش و افزایش سطح فسفر خاک غلظت آرسنیک را در بخش هوایی گیاه آفتابگردان افزایش می‌دهد. بنابراین همزیستی مایکوریزایی بوسیله گیاه آفتابگردان بازدهی تثبیت گیاهی و افزایش غلظت فسفر در خاک بازدهی جذب گیاهی آرسنیک را در خاک‌های آلوده به آرسنیک افزایش می‌دهد.

کلیدواژه‌ها

• آرسنیت
• آرسنات
• تثبیت گیاهی
• گیاه پالایی
• مایکوریزا

20.1001.1.20084757.1395.30.5.18.6

عنوان مقاله [English]

The Effects of Arbuscular-Mycorrhizal Fungi and Phosphorous on Arsenic Uptake by Sunflower Plant in Soils Spiked with Arsenite and Arsenate

نویسندگان [English]

• Saeed Bagherifam
• A. Lakzian
• A. Fotovat
• R. Khorasani

Ferdowsi University of Mashhad

چکیده [English]

Introduction: Arsenic is a highly toxic metalloid in group 15 of periodic table. The information on environmental behaviour of arsenic, however, is still scarce. Contamination of soils and water with arsenic and antimony due to their widespread industrial application and mining activities has raised serious environmental concerns. Nearly all Arsenic-contaminated soils results from human activities and it has different environmental and sociological impacts. Various strategies and methods have been proposed for environmental management and remediation of contaminated soils. Among all methods, the phytoremediation is receiving more attention due to its cost effective and environmental friendly characteristics. In the case of arsenic contaminated soils, there are effective factors such as soil fertility, nutrients content and microorganisms function, which can improve the uptake of As by plants. Up to now, several studies have been evaluated the effects of symbiotic fungal association in plants on increasing nutrients and toxic elements uptake. Many of authors reported that the mycorrhizal symbiosis increases the uptake of toxic elements in root and shoot of plants and consequently improve the efficacy of phytostabilization and phytoextraction processes. There are conflicting results about the effect of arbuscular- mycorrhizal fungi (AMF) on As uptake by various plants. Chen et al. (4) found that Glomus mosseae symbiosis with plant reduces As concentration and enhance phosphorus content in shoot and root of plant. Whilst Cozzolino et al. (7) reported that the AMF increases as concentration in shoot and root of cabbage. Phosphorus has important role on mycorrhizal symbiosis and also As uptake by plants. Therefore, current study was conducted to evaluated effect of Glomus intraradices and Glomus mosseae symbiosis with sunflower and also soil phosphorus concentration on uptake of arsenic from arsenite and arsenate contaminated soils.
Materials and Methods:The soil sample (Typic Haplorthids) was collected, air dried and passed through 2 mm sieve and then were heated in 80 centigrade degree temperature for two times. A pot experiment was conducted in a completely randomized design with factorial arrangement and three replications in greenhouse condition. The experimental factors included two species spices of inorganic As (50 mg kg-1 of Arsenite and Arsenate), two levels of phosphorus (0 and 60 mg Kg-1) and three spices of arbuscular mycorrhizae (control, Glomus intraradices and Glomus mosseae). Soil samples spiked with Na2HAsO4.7H2O, NaAsO2 (Arsenite and Arsenate) and Ca (H2PO4)2 (phosphorus) and incubated in greenhouse condition for 4 week. Sunflower seeds were planted and seedlings harvested after 60 day of sowing and then dry weight of sunflower, concentration of As and phosphorus in shoot and root of plant and root colonization percentage determined using standard methods.
Results and Discussion:The results revealed that Glomus intraradices (GI) and Glomus mosseae (GM) symbiosis significantly (P

کلیدواژه‌ها [English]

• Arsenite
• Arsenate
• phytostabilization
• phytoremediation
• Mycorrhizae Fungi

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