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نوع مقاله : مقالات پژوهشی

نویسندگان

1 گروه علوم خاک دانشگاه علوم کشاورزی و منابغ طبیعی گرگان

2 دانشگاه علوم کشاورزی و منابع طبیعی گرگان

3 دانشگاه شاهد

چکیده

یکی از راه­های بهبود ویژگی شیمیایی و حاصلخیزی خاک­های آهکی، کاربرد مواد آلی از جملـه زغال زیستی تولید شده از ضایعات آلی است. اما زغال­های زیستی عمدتاً دارای pH قلیایی بوده و کاربرد مقادیر زیاد آنها می­تواند کمبود برخی عناصر غذایی را برای گیاه در خاک­های آهکی تشدید کند. اصلاح زغال زیستی با اسیدها باعث افزایش در دسترس بودن عناصر غذایی گیاهان در خاک‌های آهکی می‌شود. هدف از این پژوهش بررسی اثر زغال زیستی اصلاح شده با اسید از کاه برنج بر مقدارکلروفیل و غلظت عناصرکم مصرف گیاه کینوا (Chenopodium quinoa) (رقم گیزاوان) در یک خاک آهکی بود. به همین منظور آزمایشی در شرایط گلخانه­ای به­صورت فاکتوریل در قالب طرح کاملاً تصادفی در 4 تکرار به ‌صورت گلدانی اجرا شد. فاکتورها شامل 3 نوع زغال زیستی (اصلاح نشده، اصلاح شده با روش پیش اسیدی و اصلاح شده با روش پس اسیدی) و مقادیر مختلف مصرف زغال زیستی (0، 2 و 5 درصد وزنی) و در مجموع 36 گلدان بودند. مقایسه میانگین اثر تیمارهای مورد بررسی نشان داد با افزایش مقدار مصرف هر سه نوع زغال زیستی مقدار کلروفیل a، b، کل و کارتنوئید افزایش یافت به نحوی که بیشترین مقدار کلروفیل a، b، کل و کارتنوئید به ترتیب با میانگین 58/2، 54/1، 13/4 و 36/1 میلی­گرم بر گرم وزن تازه گیاه از تیمار 5 درصد زغال زیستی پس اسیدی بدست آمد. همچنین بیشترین غلظت آهن، روی، مس و منگنز در اندام هوایی به‌ترتیب با میانگین 48/229، 42/13، 85/3 و 37/23 میلی‌گرم بر کیلوگرم مربوط به تیمار 5 درصد زغال زیستی پس­اسیدی بود که نسبت به تیمار 5 درصد زغال زیستی پیش اسیدی به‌ترتیب افزایشی معادل 08/4، 24/13، 44/7 و 76/30 درصد داشت. به طور کلی نتایج این پژوهش نشان داد کاربرد زغال زیستی اسیدی (پس اسیدی) می­تواند به­عنـوان روشـی مناسب برای ارتقاء حاصـلخیزی و فراهمی عناصر کم مصرف در خـاک­های آهکی مورد استفاده قرار گیرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Acid-modified Biochar Effect on Some Physiological Indicators and Micronutrient Availability of Quinoa (cv. Gizavan) in a Calcareous Soil

نویسندگان [English]

  • M. Bazi Abdoli 1
  • M. Barani Motlagh 2
  • A. Bostani 3
  • T. Nazari 2

1 Gorgan University of Agriculture and Natural Resources

2 Gorgan University of Agriculture and Natural Resources

3 Shahed University

چکیده [English]

Introduction
Organic matter and alkaline pH are the main causes of nutrient deficiencies in calcareous soils of arid and semi-arid regions. The availability of some nutritional elements, including the micronutrients such as iron, zinc, copper, and manganese is very low in calcareous soils, although the total concentration of these elements may be relatively high.  Burning crop residues results in substantial loss of nutrients, and may lead to air pollution and human health problems. An alternative approach is to apply crop residues to soil in the form of biochar. The biochar modification with acid may increase the solubility of nutrients (P, Fe, Zn, Cu, Mn) present in biochar, thereby significant improvement in mineral nutrition of plants grown in calcareous soils. Therefore, the object of this study is to investigate the effect of acid-modified biochar from rice residues on the amount of chlorophyll and the micronutrient concentration of quinoa plant (Chenopodium quinoa) in a calcareous soil.
 
Methods and Materials
The soil was air-dried and ground to pass through a 2-mm sieve then was analyzed to determine various soil physico-chemical properties using standard methods. To achieve the aim of this study the factorial experiment was carried out based on a completely randomized design in 4 replications. Factors include 3 types of biochar (unmodified, modified by pre-acidic method and modified by post-acidic method) and different levels of biochar (0, 2, and 5% by weight). Then 10 quinoa seeds were planted in each pot at 2-cm depth which after emergence, declined to 3 plants in each pot. The pots were randomly moved twice a week during the growth period to eliminate environmental effects. Irrigation and weeding operations were performed by hand. Determination of chlorophyll content (a, b, and ab) and carotenoids were measured precisely before harvesting in fresh plants using Arnon method.  Plants were harvested at 187 days after planting, washed with distilled water and dry with tissue paper. The samples were air-dried and then oven dried at 65˚C to a constant weight in a forced air-driven oven. Then the total micronutrient content of the plant was determined after dry ashing. The statistical results of the data were analyzed using SAS software (9.4) and LSD test (at 5% level) was used for comparing the mean values.
 
Results and Discussion
Based on the variance analysis, all attributes responded positively to different types and levels of biochar and modified biochar (p<0.01). The comparison of the average effect of the studied treatments showed that with the increase in the levels of all three types of biochar, the amount of chlorophyll a, b, total, and carotenoid increased so the highest amount of chlorophyll a, b, total, and carotenoid respectively, with an average of 2.58 and 1.54, 4.13 and 1.36 mg g-1 were obtained from the treatment of 5% post-acidic biochar. The results showed that the highest amount of Fe concentration in shoots with an average of 229.48 mg kg-1 was obtained from the treatment of 5% post-acidic biochar, although there was no statistically significant difference with the treatment of 5% pre-acidic biochar with an average of 220.48 mg kg-1 and its lowest value with an average of 95.95 mg kg-1 was related to unmodified biochar. The highest amount of Zn concentration in shoots with an average of 13.42 mg kg-1 was related to the treatment of 5% post-acidic biochar which showed an increase of 13.24 and 33.26% compared to the treatment of 5% pre-acidic and unmodified biochar, respectively. Also, the highest concentrations of Cu and Mn in shoots were obtained with an average of 3.85 and 23.37 mg kg-1 respectively, from the treatment of 5% post-acidic biochar.
 
Conclusion
Post-acidic biochar had better results in terms of physiological indices and the concentration of micronutrients (Fe, Zn, Cu, and Mn) than unmodified biochar in quinoa. The increase of nutrients in quinoa can be attributed to the dissolution of biochar nutrients after being modified with acid and the reduction of pH and the availability of these elements in the soil. Therefore, biochar modified with acid or biochar produced from sources that have acidic properties can be recommended as a suitable method for improving fertility and increasing micronutrients in calcareous soils affected by salt.
 

کلیدواژه‌ها [English]

  • Carotenoid
  • Chlorophyll
  • Post-acidic biochar
  1. Abbasi, G.H., Akhtar, J., Anwar-ul-Haq, M., Malik, W., Ali, S., Chen, Z.H., & Zhang, G. (2015). Morpho-physiological and micrographic characterization of maize hybrids under NaCl and Cd stress. Plant Growth Regulation75, 115-122. https://doi.org/10.1007/s10725-014-9936-6
  2. Abdel-Fattah, M.K. (2019). Reclamation of saline-sodic soils for sustainable agriculture in Egypt. Sustainability of Agricultural Environment in Egypt: Part II: Soil-Water-Plant Nexus, 69-92. https://doi.org/10.1007/698_2018_310
  3. Adejumo, S.A., Owolabi, M.O., & Odesola, I.F. (2016). Agro-physiologic effects of compost and biochar produced at different temperatures on growth, photosynthetic pigment and micronutrients uptake of maize crop. African Journal of Agricultural Research11(8), 661-673.
  4. Allah Vardi Markdeh and longevity. (2014). Investigating the effect of copper and manganese on the amount of secondary compounds of lemongrass medicinal plant (Mellisa officinalis), international conference on applied research in agriculture. (In Persian)
  5. Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgarisPlant Physiology24(1), 1.
  6. Benton Jones, J.R., & Case, V.W. (1990). Sampling, handling and analyzing plant tissue sample. Soil Testing and Plant Analysis, SSSA, (3).
  7. Boostani, H.R., Zarei, M., & Barati, V. (2017). Effects of application of biochar and arbuscular mycorrhizal fungi on growth and chemical composition of corn (Zea mays 704) in a calcareous soil. Journal of Soil Management and Sustainable Production, 7(2), 1-23. (In Persian with English abstract)
  8. Chan, K.Y., Van Zwieten, L., Meszaros, I., Downie, A., & Joseph, S. (2007). Agronomic values of greenwaste biochar as a soil amendment. Soil Research45(8), 629-634.
  9. Day, P.R. (1965). Particle fractionation and particle size analysis. Methods of Soil Analysis: Part 1 Physical and Mineralogical Properties, Including Statistics of Measurement and Sampling9, 545-567.
  10. Ding, W., Clode, P.L., & Lambers, H. (2019). Is pH the key reason why some Lupinus species are sensitive to calcareous soil?. Plant and Soil434, 185-201.
  11. Eichert, T., Peguero‐Pina, J.J., Gil Pelegrín, E., Heredia, A., & Fernández, V. (2010). Effects of iron chlorosis and iron resupply on leaf xylem architecture, water relations, gas exchange and stomatal performance of field grown peach (Prunus persica). Physiologia Plantarum138(1), 48-59.
  12. El-Sharkawy, M., El-Naggar, A.H., AL-Huqail, A.A., & Ghoneim, A.M. (2022). Acid-modified biochar impacts on soil properties and biochemical characteristics of crops grown in saline-sodic soils. Sustainability14(13), 8190.
  13. Farrell, M., Macdonald, L.M., Butler, G., Chirino-Valle, I., & Condron, L.M. (2014). Biochar and fertiliser applications influence phosphorus fractionation and wheat yield. Biology and Fertility of Soils50, 169-178.
  14. Fu, P., Hu, S., Xiang, J., Yi, W., Bai, X., Sun, L., & Su, S. (2012). Evolution of char structure during steam gasification of the chars produced from rapid pyrolysis of rice husk. Bioresource Technology114, 691-697.
  15. Glaser, B., Lehmann, J., & Zech, W. (2002). Ameliorating physical and chemical properties of highly weathered soils in the tropics with charcoal–a review. Biology and Fertility of Soils35, 219-230.
  16. Hasanpour, I., Shirvani, M., Hajabbasi, M.A., & Majidi, M.M. (2022). Effect of acidic biochars on some chemical properties and nutrient availabilities of calcareous soils. JWSS-Isfahan University of Technology26(2), 39-59. (In Persian)
  17. He, Z., He, C., Zhang, Z., Zou, Z., & Wang, H. (2007). Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. Colloids and Surfaces B: Biointerfaces59(2), 128-133.
  18. Inal, A., Gunes, A.Y.D.I.N., Sahin, O.Z.G.E., Taskin, M.B., & Kaya, E.C. (2015). Impacts of biochar and processed poultry manure, applied to a calcareous soil, on the growth of bean and maize. Soil Use and Management31(1), 106-113.
  19. Jacobsen, S.E., Hollington, P.A., & Hussain, Z. (2002). Quinoa (Chenopodium quinoa), a potential new crop for Pakistan. Prospects for Saline Agriculture, 247-249.
  20. Jin, Z., Chen, C., Chen, X., Hopkins, I., Zhang, X., Han, Z., & Billy, G. (2019). The crucial factors of soil fertility and rapeseed yield-A five year field trial with biochar addition in upland red soil, China. Science of the Total Environment649, 1467-1480.
  21. Jones Jr, J.B., & Case, V.W. (1990). Sampling, handling, and analyzing plant tissue samples. Soil Testing and Plant Analysis3, 389-427.
  22. Kappler, A., Wuestner, M. L., Ruecker, A., Harter, J., Halama, M., & Behrens, S. (2014). Biochar as an electron shuttle between bacteria and Fe (III) minerals. Environmental Science & Technology Letters1(8), 339-344.
  23. Komkiene, J., & Baltrenaite, E. (2016). Biochar as adsorbent for removal of heavy metal ions [Cadmium (II), Copper (II), Lead (II), Zinc (II)] from aqueous phase. International Journal of Environmental Science and Technology13, 471-482
  24. Lahiji, F.A.S., Ziarati, P., & Jafarpour, A. (2016). Potential of rice husk biosorption in reduction of heavy metals from Oryza sativa Biosciences Biotechnology Research Asia, 13, 2231-2237.
  25. Lahiji, F.A.S., Ziarati, P., & Jafarpour, A. (2016). Potential of rice Husk biosorption in reduction of heavy metals from Oryza sativaBiosciences Biotechnology Research Asia13(4), 2231-2237.
  26. Lehmann, J., & Rondon, M. (2006). Bio-char soil management on highly weathered soils in the humid tropics. Biological Approaches to Sustainable Soil Systems113(517), e530.
  27. Lehmann, J., Pereira da Silva, J., Steiner, C., Nehls, T., Zech, W., & Glaser, B. (2003). Nutrient availability and leaching in an archaeological Anthrosol and a Ferralsol of the Central Amazon basin: fertilizer, manure and charcoal amendments. Plant and Soil249, 343-357.
  28. Lindsay, W.L., & Norvell, W. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America Journal42(3), 421-428.
  29. Mahmoudi, H., Ksouri, R., Gharsalli, M., & Lachaâl, M. (2005). Differences in responses to iron deficiency between two legumes: lentil (Lens culinaris) and chickpea (Cicer arietinum). Journal of Plant Physiology162(11), 1237-1245.
  30. Martínez, E.A., Fuentes, F.F., & Bazile, D. (2015). History of quinoa: its origin, domestication, diversification, and cultivation with particular reference to the Chilean context. Quinoa: Improvement and Sustainable Production, 19-24.
  31. Mayer, Z.A., Eltom, Y., Stennett, D., Schröder, E., Apfelbacher, A., & Hornu, A. (2014). Characterization of engineered biochar for soil management. Environmental Progress & Sustainable Energy, 33, 490–96. https://doi.org/10.1002/ep.11788
  32. Ming-gang, X.U., Dong-chu, L.I., Ju-mei, L.I., Dao-zhu, Q.I.N., Yagi, K., & Hosen, Y. (2008). Effects of organic manure application with chemical fertilizers on nutrient absorption and yield of rice in Hunan of Southern China. Agricultural Sciences in China, 7(10), 1245-1252.
  33. Nael, M., Khademi, H., Jalalian, A., Schulin, R., Kalbasi, M., & Sotohian, F. (2009). Effect of geo-pedological conditions on the distribution and chemical speciation of selected trace elements in forest soils of western Alborz, Iran. Geoderma152(1-2), 157-170.
  34. Naroi, A., Zamani, J., Kohestani, Sh., & Abbaszadeh Afshar, F. (2022). The effect of date leaf biochar and pistachio harvest waste biochar on corn growth and heavy metal concentration. Agricultural Engineering, 4, 44. 399-411. (In Persian)
  35. Novak, J.M., Busscher, W.J., Laird, D.L., Ahmedna, M., Watts, D.W., & Niandou, M.A. (2009). Impact of biochar amendment on fertility of a southeastern coastal plain soil. Soil Science174(2), 105-112.
  36. Olsen, S.R. (1954). Estimation of available phosphorus in soils by extraction with sodium bicarbonate(No. 939). US Department of Agriculture.
  37. Page, A.L., Miller, R.H., & Keeney, D.R. (1982). Methods of soil analysis: chemical and microbiological properties, 2nd edn. American Society of Agrronomy Inc, Madison.
  38. Peiris, C., Nayanathara, O., Navarathna, C. M., Jayawardhana, Y., Nawalage, S., Burk, G., ... & Gunatilake, S.R. (2019). The influence of three acid modifications on the physicochemical characteristics of tea-waste biochar pyrolyzed at different temperatures: a comparative study. RSC Advances9(31), 17612-17622.
  39. Ramzani, P.M.A., Khan, W.U.D., Iqbal, M., Kausar, S., Ali, S., Rizwan, M., & Virk, Z.A. (2016). Effect of different amendments on rice (Oryza sativa) growth, yield, nutrient uptake and grain quality in Ni-contaminated soil. Environmental Science and Pollution Research23, 18585-18595.
  40. Ramzani, P.M.A., Shan, L., Anjum, S., Ronggui, H., Iqbal, M., Virk, Z.A., & Kausar, S. (2017). Improved quinoa growth, physiological response, and seed nutritional quality in three soils having different stresses by the application of acidified biochar and compost. Plant Physiology and Biochemistry, 116, 127-138. https://doi.org/1016/j.plaphy.2017.05.003
  41. Ran, C., Gulaqa, A., Zhu, J., Wang, X., Zhang, S., Geng, Y., ... & Shao, X. (2020). Benefits of biochar for improving ion contents, cell membrane permeability, leaf water status and yield of rice under saline–sodic paddy field condition. Journal of Plant Growth Regulation39(1), 370-377.
  42. Safi, D., Alikhani, H., & Motsharazadeh, B. (2012). The effect of plant growth promoting rhizobial bacteria on improving the nutritional conditions of rapeseed (Barssica napus) under salt stress. Science of Water and Soil, 23(4): 159-175. (In Persian)
  43. Sahin, O., Taskin, M.B., Kaya, E.C., Atakol, O.R.H.A.N., Emir, E., Inal, A., & Gunes, A.Y.D.I.N. (2017). Effect of acid modification of biochar on nutrient availability and maize growth in a calcareous soil. Soil Use and Management33(3), 447-456.
  44. Sharifian Bahraman, A., Sepehry, A., & Barani, H. (2022). Soil physiochemical characteristics of Lycium depressum Stocks. habitat in saline and alkaline rangelands in north of Golestan Province, Iran. Journal of Plant Ecosystem Conservation9(19), 47-62. (In Persian)
  45. Silber, A., Levkovitch, I., & Graber, E.R. (2010). pH-dependent mineral release and surface properties of cornstraw biochar: agronomic implications. Environmental Science & Technology44(24), 9318-9323.
  46. Song, W., & Guo, M. (2012). Quality variations of poultry litter biochar generated at different pyrolysis temperatures. Journal of Analytical and Applied Pyrolysis, 94, 138-145.
  47. Taskin, M. B., Kadioglu, Y. K., Sahin, O., Inal, A., & Gunes, A. (2019). Effect of acid modified biochar on the growth and essential and non-essential element content of bean, chickpea, soybean, and maize grown in calcareous soil. Communications in Soil Science and Plant Analysis50(13), 1604-1613.
  48. Thomas, S.C., Frye, S., Gale, N., Garmon, M., Launchbury, R., Machado, N., ... & Winsborough, C. (2013). Biochar mitigates negative effects of salt additions on two herbaceous plant species. Journal of Environmental Management129, 62-68.
  49. Vithanage, M., Rajapaksha, A.U., Zhang, M., Thiele-Bruhn, S., Lee, S.S., & Ok, Y.S. (2015). Acid-activated biochar increased sulfamethazine retention in soils. Environmental Science and Pollution Research22, 2175-2186. https://doi.org/1007/s11356-014-3434-2
  50. Walkley, A., & Black, I.A. (1934). An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science37(1), 29-38.
  51. Wong, V.N., Greene, R.S.B., Dalal, R.C., & Murphy, B.W. (2010). Soil carbon dynamics in saline and sodic soils: a review. Soil Use and Management26(1), 2-11.
  52. Woolf, D., Amonette, J.E., Street-Perrott, F.A., Lehmann, J., & Joseph, S. (2010). Sustainable biochar to mitigate global climate change. Nature Communications1(1), 56.
  53. Yazdanpanah, A., & Motallebifard, R. (2017). The effects of chicken manure and potassium fertilizer on Yield and Nitrogen, Phosphorus, Potassium, Zinc and Copper Uptake of Potato. Applied Soil Research4(2), 60-71. (In Persian)
  54. Yuan, J.H., & Xu, R.K. (2011). The amelioration effects of low temperature biochar generated from nine crop residues on an acidic Ultisol. Soil Use and Management27(1), 110-115.
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