دوماه نامه

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه فردوسی مشهد

2 دانشگاه آزاد اسلامی واحد کرج

چکیده

بازدارنده‌های نیترات­زایی ترکیباتی هستند که اکسایش زیستی آمونیوم به نیتریت را به تأخیر می‌اندازند و بدین ترتیب تجمع نیترات در خاک را کاهش می‌دهند. این پژوهش به منظور بررسی تاثیر بازدارنده‌های نیترات‌زایی، 3 و 4-دی متیل پیرازول فسفات (DMPP)، دی­سیان­دی­آمید (DCD) و پودر درمنه (ART)، در حضور منابع مختلف نیتروژن، بر تغییرات نیتروژن معدنی خاک، میزان نیتروژن گیاه، تجمع نیترات در برگ گیاه و برخی از خصوصیات رشدی کاهو، در قالب طرح پایه کاملا تصادفی با آرایش فاکتوریل در سه تکرار انجام شد. نتایج نشان داد که بازدارنده‌های نیترات­زایی در حضور منابع مختلف کود نیتروژن موجب کاهش غلظت نیتروژن نیتراتی و افزایش نیتروژن آمونیومی خاک شدند. همچنین غلظت نیتروژن در گیاه در حضور بازدارنده­های نیترات­زایی افزایش یافت. کاربرد بازدارنده‌های نیترات­زایی موجب کاهش غلظت نیترات در برگ گیاه شد. همین­طور استفاده از بازدارنده­های نیترات­زایی بر خصوصیات رشدی گیاه نیز تاثیر گذاشتند. به طور کلی بهترین کارکرد بازدارنده‌های نیترات‌زایی در حضور منبع کود اوره مشاهده شد و بازدارنده نیترات‌زایی ART دارای بیشترین تاثیر و پس از آن بازدارنده‌های DMPP و DCD دارای بهترین کارکرد بودند.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Artificial and Natural Nitrification Inhibitors on Plant Growth Characteristics and Nitrate Uptake by Lettuce

نویسندگان [English]

  • S. Sangsefidi 1
  • A. Lakzian 1
  • A.R. Astaraei 1
  • M. Banayan 1
  • M. Mazhari 2

1 Ferdowsi University of Mashhad

2 Karaj

چکیده [English]

Introduction: Nitrification inhibitors are compounds that slow biological oxidation of ammonium to nitrite by reducing the activity of Nitrosomonas bacteria, without affecting the subsequent oxidation of nitrite to nitrate, either by inhibiting or interfering with the metabolism of nitrifying bacteria. The first step of nitrification is inhibited (i.e., the activity of Nitrosomonas bacteria) by the nitrification inhibitors, while the second step for oxidation of nitrite (NO2-) to nitrate (NO3-) is normally not influenced. In recent years, numerous compounds have been identified and used as nitrification inhibitors, particularly in agricultural soils. They are chemical compounds that slow the nitrification of ammonia, ammonium-containing, or urea-containing fertilizers, which are applied to soil as fertilizers, such as  thiourea, carbon Sulfide,  thioethers, ethylene, 3-amino-1,2,4-triazole, dicyandiamide (DCD), 2-amino-4-chloro-6-methyl pyrimidine, ammonium thiosulphate and 3,4-dimethylpyrazole phosphate (DMPP). These inhibitors reduce the losses of nitrogen in soil. Some nitrification inhibitors are very effective in the efficiency of the nitrogen fertilizers. Recently, a lot of attention has been paid to nitrification inhibitors from an environmental point of view. Some nitrification inhibitors are very expensive and not economically suitable for land application. Nonetheless, many farmers and researchers apply these compounds for many purposes in some specific places. On the other hand, there are many inexpensive natural nitrification inhibitors such as Artemisia powder, Karanj (Pongamia glabra), neem (Azadrachta indica) and tea (Camellia sinensis) waste which can compete with the artificial nitrification inhibitors such as 3, 4-dimethylpyrazole phosphate (DMPP), dicyandiamide (DCD) which are very common nitrification inhibitors. Applying 1.5 kg ha-1 of DMPP is sufficient to achieve optimal nitrification inhibition. 4-dimethylpyrazole phosphate (DMPP) can significantly shrink nitrate (NO3) leaching. 4-dimethylpyrazole phosphate (DMPP) may also decrease N2O emission and the use of DMPP-containing fertilizers can improve yield. The aim of this study was to compare the effect of 3, 4-dimethylpyrazole phosphate (DMPP), Dicyandiamide (DCD) and powder Artemisia (ART) at the presence of Urea, cow manure and Vermicompost.
Material and Methods: Effects of three nitrification inhibitors, (3, 4-dimethylpyrazole phosphate (DMPP), Dicyandiamide (DCD) and powder Artemisia (ART)) at the presence of three nitrogen sources (Urea, cow manure and Vermicompost) were investigated in a calcareous soil under lettuce cultivation in a greenhouse condition.  The changes in the soil mineral nitrogen (nitrate and ammonium), plant nitrogen, nitrate accumulation in leaves and some of growth characteristics such as lettuce chlorophyll content, leaf area index, leaf dry weight and root dry weight were determined. The experiment was carried out in a completely randomized factorial design with three replications. Soil ammonium and nitrate concentration were measured during the experiment. The growth characteristics of lettuce were also measured at the end of experiment. Nitrogen and nitrate contents were also determined in lettuce leaves. 
Results and Discussion: The results of the experiment showed that soil nitrate decreased at the presence of three nitrification inhibitors but the soil nitrogen ammonium increased significantly. Application of nitrification inhibitors also reduced the concentration of nitrate in the lettuce leaves during two harvesting times. Moreover, the nitrogen concentration in the plant increased at the presence of nitrification inhibitors. The application of nitrification inhibitors influenced the plant growth characteristics and changed the lettuce growth characteristics. Chlorophyll content increased significantly in lettuce leaves. Leaf area index, leaf and root dry weight of lettuce increased notably when 3, 4-dimethylpyrazole phosphate (DMPP) and powder Artemisia (ART) nitrification inhibitors were applied to the soil samples. These growth characteristics, however, reduced significantly when dicyandiamide nitrification inhibitors was applied to the soil samples. In addition, the symptoms of toxicity were observed in lettuce plant when dicyandiamide nitrification inhibitors were applied to the soil samples. In general, the highest efficiency of nitrification inhibitors was recorded at the presence of urea fertilizer source and the greatest efficiency was observed initially for powder Artemisia (ART) and then for 3, 4-dimethylpyrazole phosphate (DMPP) and dicyandiamide, respectively, when urea fertilizer was applied to the soil samples. There was a positive correlation between soil nitrogen content and plant nitrate in the first and second harvest. The correlation between soil ammonium and plant nitrate (in the first and second harvest) and soil nitrate was negative.

کلیدواژه‌ها [English]

  • Nitrification inhibitors
  • Lettuce
  • Urea
  • Cow manure
  • Vermicompost
1- Abalos D., Jeffery S., Sanz-Cobena A., Guardia G., and Vallejo A. 2014. Meta-analysis of the effect of urease and nitrification inhibitors on crop productivity and nitrogen use efficiency. Agriculture, Ecosystems & Environment 189: 136-144.
2- Ahmed A.A., Abdel-Baky M.M.H., and Abdel-Aal F.S. 2004. The productivity of jew's mallow plant as influenced by different NK fertilization. Mansoura University Journal of Agricultural Sciences (Egypt).
3- Alderfasi A.A., Aljuaid A.M., Moftah A.E., and Selim M.M. 2015. IRole of Nitrification Inhibitor Combined with Different Nitrogen Sources in Decreasing Injurious Components in Spinach. Advance Plants Agriculture Research 2(6): 10-15.
4- Ali I.E.A., Kafkafi U., Yamaguchi I., Sugimoto Y., and Inanaga S. 1998. Response of oilseed rape plant to low root temperature and nitrate: ammonium ratios. Journal of Plant Nutrition 21(7): 1463-1481.
5- Amberger A., and Vilsmeier K. 1979. Dicyandiamidabbau in Quarzsand und Böden. Journal of Plant Nutrition and Soil Science 142(6): 778-785.
6- Asing J., Saggar S., Singh J., and Bolan N.S. 2008. Assessment of nitrogen losses from urea and an organic manure with and without nitrification inhibitor, dicyandiamide, applied to lettuce under glasshouse conditions. Soil Research 46(7): 535-541.
7- Beritto D.T., and Kronzucker H.J. 2002. NH4+ toxicity in higher plants. Journal Plant Physiology 159: 567-584.
8- Blanco F.F., and Folegatti M.V. 2005. Estimation of leaf area for greenhouse cucumber by linear measurements under salinity and grafting. Scientia Agricola 62(4): 305-309.
9- Bremner J.M., and Mulvaney C.S. 1982. Nitrogen – total. Methods of soil analysis. Part 2. Chemical and Microbiological Properties 2(1): 595-624.
10- Chapman H.D. 1965. Total exchangeable bases. Methods of soil analysis. Part 2. Chemical and microbiological properties.
11- Crawford D.M., and Chalk P.M. 1993. Sources of N uptake by wheat (Triticum aestivum L.) and N transformation in soil treated with a nitrification inhibitor (nitrapyrin). Plant and Soil 149: 59-72.
12- Fangueiro D., Fernandes A., Coutinho J., Moreira N., and Trindade H. 2009. Influence of two nitrification inhibitors (DCD and DMPP) on annual ryegrass yield and soil mineral N dynamics after incorporation with cattle slurry. Communications in Soil Science and Plant Analysis 40: 3387-3398.
13- Gent M.P.N. 2002. Growth and composition of salad greens as affected by organic compared to nitrate fertilizer and by environment in high tunnels. Journal of plant Nutrition 25(5): 981-998.
14-Guillaumes E., and Villar J.M. 2004. Effects of DMPP on growth and chemical composition of ryegrass (Lolium perenne L.) raised on calcareous soil. Spanish Journal of Agricultural Research 2: 588-596.
15-Hähndel R., and Zerulla W. 1999, August. Effects of ammonium-stabilized N-fertilizers on yield and quality of vegetables. In International Conference on Environmental Problems Associated with Nitrogen Fertilisation of Field Grown Vegetable Crops 563: 81-86.
16-Haynes R.J. 1986. Uptake and assimilation of mineral nitrogen by plants. Mineral nitrogen in the plant-soil system, 303-378 pp.
17-Imami A. 1996. Explanation of plant decomposition methods, Vol. 1, Technical journal No. 982, Tehran University of Tehran. 110 p. (In Persian)
18-Islam N. 1991. A comparative study of the role of nitrification inhibitors in soil with special attention to their potentials and limitations.
19-Kaffashani A., Riahi M., Entezari M., Hassanzadeh A., Mohabat L., And Torabi A.H. 2013. Comparison of Nitrate content in irrigated vegetables with Zayandeh Rood water and well water. Health Research Journal 9(2): 201-196 (In Persian with English abstract)
20- Keeney D.R.A., and Nelson D. 1982. Nitrogen –inorganic forms. Methods of soil analysis. Part 2. Chemical and microbiological properties, (methods of soil an 2), 643- 698pp.
21-Keeney D.R. 1986. Inhibition of nitrification in soils.
22- Kiani Sh. 2010. Use of nitrification inhibitors to increase nitrogen use efficiency, improve the quality of agricultural products and preserve the environment. First congress on fertilizer challenges in Iran. University of Shahr Kord. (In Persian)
23- Kiran U., and Patra D.D. 2002. Influence of natural essential oils and synthetic nitrification inhibitors on crop yield and nitrogen use efficiency in mint (Mentha arvensis L.)- mustard (Brassica juncea L.) cropping sequence. Journal of the Indian Society of Soil Science 50(1): 64-69.
24- Kiran U., and Patra D. 2003a. Medicinal and aromatic plant materials as nitrification inhibitors for augmenting yield and nitrogen uptake of Japanese mint (Mentha arvensis L. Var. Piperascens). Bioresource Technology 86: 267-276.
25- Kiran U., and Patra D.D. 2003b. Influence of natural essential oils and their by-products as nitrification retarders in regulating nitrogen utilization for Japanese mint in sandy loam soils of subtropical central India. Agriculture, Ecosystems & Environment 94(2): 237-245.
26- Leghari S.J., Wahocho N.A., Laghari G.M., HafeezLaghari A., MustafaBhabhan G., HussainTalpur K., Bhutto T.A., Wahocho S.A., and Lashari A.A. 2016. Role of nitrogen for plant growth and development: a review. Advances in Environmental Biology 10(9): 209-219.
27- Linzmeier W., Schmidhalter U., and Gutser R. 2001. Effect of DMPP on nitrification and N losses (nitrate, NH3, N2O) from fertilizer nitrogen in comparison to DCD. In VDLUFA-Institution Series Congress (52): 485-488.
28- Liu C., Wang K., and Zheng X. 2013. Effects of nitrification inhibitors (DCD and DMPP) on nitrous oxide emission, crop yield and nitrogen uptake in a wheat-maize cropping system. Biogeosciences 10(4): p.2427.
29- Macadam X.M.B., del Prado A., Merino P., Estavillo J.M., Pinto M., and Gonzalez-Murua C. 2003. Dicyandiamide and 3, 4-dimethyl pyrazole phosphate decrease N2O emissions from grassland but dicyandiamide produces deleterious effects in clover. Journal of Plant Physiology 160(12): 1517-1523.
30- Martinez-Alcantara B., Quiñones A., Polo C., Primo-Millo E., and Legaz F. 2013. Use of nitrification inhibitor DMPP to improve nitrogen uptake efficiency in citrus trees. Journal of Agricultural Science 5(2): 10-16.
31- Mengel K., and Kirkby E.A. 2001. Principles of Plant Nutrition. 5th Kluwer Academic of mixed agricultural landuse. Enviromental Pollution 115: 191-204
32- Murugan R.A., Chitraputhirapillai S.W., von Fragstein N.P., and Nanjappan K. 2011. Effects of combined application of biofertilisers with neem cake on soil fertility, grain yield and protein content of black gram (Vigna mungo (L.) Hepper). World Journal of Agricultural Sciences 7(5): 583-590.
33- Olsen S.R., Cola C.V., Watanabe F.S., and Dean C.A. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. U. S Departmene of Agriculture, Cirular, 939pp.
34- Page A.L., Miller R.H., and Keeny D.R. 1982. Methods of soil analysis. Part 2. Chemical and microbiological properties: American Society of Agronomy, Inc. Soil Science Society of Aamerica, Madison, WI.
35- Panakova Z., Slamka P., and Lozek O. 2016. Effect of nitrification inhibitors on the content of available nitrogen forms in the soil under maize (Zea mays L.) growing. Journal of Central European Agriculture 17(4): 1013-1032.
36- Pasda G., Hähndel R., and Zerulla W. 2001. Effect of fertilizers with the new nitrification inhibitor DMPP (3, 4-dimethylpyrazole phosphate) on yield and quality of agricultural and horticultural crops. Biology and Fertility of Soils 34(2): 85-97.
37- Premuzic Z., Garate A., and Bonilla I. 2000. Production of lettuce under different fertilisation treatments, yield and quality. In Workshop Towards and Ecologically Sound Fertilisation in Field Vegetable Production. 65-72pp.
38- Raper C.D., Smith W.T., and York E.K. 1974. Geometry of tobacco leaves: effect on estimation of leaf area. Tobacco international.
39- Randall G.W., Vetsch J.A., and Huffman J.R. 2003. Corn production on a subsurface-drained mollisol as affected by time of nitrogen application and nitrapyrin. Agronomy Journal 95(5): 1213-1219.
40- Randall G.W., and Vetsch J.A. 2005. Corn production on a subsurface-drained mollisol as affected by fall versus spring application of nitrogen and nitrapyrin. Agronomy Journal 97(2): 472-478.
41- Reeves D.W., and Touchton J.T. 1986. Relative phytotoxicity of dicyandiamide and availability of its nitrogen to cotton, corn, and grain sorghum. Soil Science Society of America Journal 50: 1353-1357.
42- Shariati M., And Madadkar Haghjo M. . Plant physiology: absorption and transfer of material through the membrane. Isfahan University Press. 65 p. (In Persian)
43- Sarkar A., Sarkar S., and Zaman A. 2011. Growth and yield of potato as influenced by combination of organic manures and inorganic fertilizers. Potato Journal 38(1): 25-36.
44- Serna M.D., Banuls J., Quinones A., Primo-Millo E., and Legaz F. 2000. Evaluation of 3, 4-dimethylpyrazole phosphate as a nitrification inhibitor in a Citrus-cultivated soil. Biology and Fertility of Soils 32(1): 41-46.
45- Shamsuzzaman S.M., Hanafi M.M.H., Samsuri A.W., Halimi S.M., Begum M., and Maisarah J.N. 2016. Impact of nitrification inhibitor with organic manure and urea on nitrogen dynamics and N2O emission in acid sulphate soil. Bragantia 75(1):108-117.
46- Sivasakthy K., and Gnanavelrajah N. 2012. Organic Nitrogen Sources and Nitrification Inhibitors on Leaching and Phyto-Accumulation of Nitrate and Yield of Amaranthus polygamous. World Journal of Agricultural Sciences 8(2): 208-211.
47- Subbarao G.V., Ito O., Sahrawat K.L., Berry W.L., Nakahara K., Ishikawa T., Watanabe T., Suenaga K., Rondon M., and Rao I.M. 2006. Scope and strategies for regulation of nitrification in agricultural systems—challenges and opportunities. Critical Reviews in Plant Sciences 25(4): 303-335.
48- Taiz L., and Zeiger E. 1991. Plant Physiology The Benjamin. Cummings Redwood City, 565pp.
49- Tate K.R. 1985. Soil phosphorus. In Soil organic matter and biological activity (pp. 329-377). Springer Netherlands.
50- Tilak K.V.B.R., Singh C.S., Roy N.K., and Subba Rao N.S. 1992. Azospirillum brasilense and Azotobacter chrococcum inoculum effect on maize and sorghum. Soil Biology and Biochemistry, 14, pp.417-418.Thind, H., O. Choudhary, R. Guota, and M. Vashista. 2013.
51- Trenkel M.E. 1997a. Improving fertilizer use efficiency controlled realease and stabilized fertilizers in agriculture. Paris International Fertilizer Industry Association.
52- Upadhyay R.K., Tewari S.K., and Patra D.D. 2011. Natural nitrification inhibitors for higher nitrogen use efficiency, crop yield, and for curtailing global warming. Journal of Tropical Agriculture 49: 19-24.
53- Walkley A., and Black I.A. 1934. An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science 37(1): 29-38.
54- Weiske A., Benckiser G., Herbert T., and Ottow J. 2001. Influence of the nitrification inhibitor 3, 4-dimethylpyrazole phosphate (DMPP) in comparison to dicyandiamide (DCD) on nitrous oxide emissions, carbon dioxide fluxes and methane oxidation during 3 years of repeated application in field experiments. Biology and Fertility of Soils 34(2): 109-117.
55- Xu C., and Zhang F.S. 2005. Role of nitrification inhibitor DMPP (3, 4-dimethylpyrazole phosphate) in NO_3^--N accumulation in greengrocery (Brassica campestris L. Ssp. Chinensis) and vegetable soil. Journal of Environmental Sciences 17(1): 81-83.
56- Xu L., Chen H., Xu J., Yang J., Li X., Liu M., Jiao J., Hu F., and Li H. 2014. Nitrogen transformation and plant growth in response to different urea‐application methods and the addition of DMPP. Journal of Plant Nutrition and Soil Science 177(2): 271-277.
57- Zerulla W., Barth T., Dressel J., Erhardt K., von Locquenghien K.H., Pasda G., Rädle M., and Wissemeier A. 2001. 3, 4-Dimethylpyrazole phosphate (DMPP)–a new nitrification inhibitor for agriculture and horticulture. Biology and Fertility of Soils 34(2):79-84.
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