Document Type : Research Article
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Abstract
Due to widespread distribution, hydrocarbon toxicity and mutagencity, PAHs are listed as hazardous pollutants, and remediation of soils contaminated with PAHs is a major challenge. The use of degrading microorganisms and plants for bioremediation of PAHs-contaminated environments seems to be a viable technology for restoration of polluted sites. The purpose of this research was investigation the effect of mycorrhiza and degrading bacteria in increasing pytoremediation. For this purpose, the soil deliberately contaminated with crude oil in 1 and 2 wt% rate and four treatments: plant multiflorum (T1), plant multiflorum with mycorrhiza inoculation (T2), plant multiflorum with oil degrading bacteria inoculation (T3), plant multiflorum with mycorrhiza and oil degrading bacteria inoculation (T4) were employed for bioremediation of oil contaminated soil. The study results showed that with increasing the level of pollution, shoots and roots yield was decreased. The percentage of AM colonization in mycorrhizal treatments did not significantly reduce the yield. Most importantly, degradation of oil components was significantly enhanced by the addition of oil-degrading microorganisms, compared to remediation of growing plants alone at both level of pollution. The highest oil degradation (85%) was observed with AMF + oil degrading bacteria in soil with pollution level of 2%., GC results indicated that all normal paraffin and isoperopanoids i.e. Phytane and Pristane decreased from 40 to 80 percent in treatments with oil-degrading microorganisms.
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