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نوع مقاله : مقالات پژوهشی

نویسندگان

پژوهشکده مرکبات و میوه های نیمه گرمسیری ایران

چکیده

پژوهش حاضر با هدف بررسی اثر مکش آب خاک، همراه با پتاسیم بر عملکرد، برخی ویژگی‌های بیوشیمیایی و رشد رویشی درختان پرتقال تامسون‌ناول روی پایه سیتروملو اجرا شد. بدین­منظور، یک آزمایش فاکتوریل در قالب طرح آماری بلوک کاملاً تصادفی به مدت دو سال در باغ پرتقال اجرا شد. فاکتورها شامل: انجام آبیاری در مکش­های رطوبتی خاک 20، 40، 60 کیلو پاسکال (بر اساس مکش تانسیومتر) و یک تیمار بدون آبیاری؛ و کاربرد کود پتاسیم در دو سطح 50 و 100 گرم پتاسیم ضرب‏ در سن درخت در نظر گرفته شد. سپس، در دو سال آخر آزمایش عملکرد، پتاسیم قابل ‏استفاده و قطر تاج درختان و در سال آخر آزمایش، مقدار پرولین، نشت یونی و هدایت الکتریکی خاک اندازه گیری شد. نتایج این تحقیق نشان داد که مقدار قطر تاج درختان با انجام آبیاری افزایش (05/0P<) یافت. هم­چنین، نتایج سال آخر پژوهش نشان داد که در سطح اول پتاسیم بین مقدار عملکرد در تیمارهای آبیاری 20، 40 و 60 کیلوپاسکال تفاوت معنی­داری (05/0P<) مشاهده نشد. علاوه­براین، اثر پتاسیم بر عملکرد به مکش آب خاک بستگی داشت. هم­چنین، با افزایش مکش رطوبتی خاک در زمان آبیاری مقدار پرولین، نشت یونی، پتاسیم قابل‏استفاده و قابلیت هدایت الکتریکی افزایش معنی­داری داشتند. به­طور کلی، باتوجه به نتایج عملکرد و نیز با در نظر گرفتن محدویت منابع آبی می‏توان پیشنهاد کرد که در مناطق با اقلیم و خاک مشابه با منطقه مور مطالعه، بهترین زمان آبیاری درختان پرتقال تامسون‏ناول موقعی است که تانسیومتر مکش 60 کیلوپاسکال (ضریب تخلیه 52 درصد) را نشان دهد. علاوه براین، بهینه­ترین مقدار پتاسیم جهت کوددهی این درختان در شرایط ذکر شده، معادل 50 گرم پتاسیم ضرب در سن درخت می­باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Soil Water Potential and Potassium Application Effects on Yield and Biochemical Characteristics of Thomson Navel Orange (Citrus sinensis L. Osbeck.)

نویسندگان [English]

  • tahereh raiesi
  • bijan moradi
  • Behruz Golein

Citrus and Subtropical Fruit Research Center, Horticultural Science Research Institute

چکیده [English]

Introduction: Citrus is the main fruit group grown in tropical as well as sub-tropical climate of more than 150 countries in the world. In Iran, the total area under citrus crops is 0.284 M ha with a production of 4.345 M ton and a productivity of 17 ton per ha. Citrus is also one of the most important horticultural products in Mazandaran, with 112,000ha devoted to its cultivation. Drought stress is frequent in Iran and is common in the dry summer periods in Mazandaran. Therefore, irrigation is essential during mentioned periods in this province. Irrigation scheduling and water requirement of the citrus crops are one of the main concerns of the citrus fruit production. Irrigating based on soil water potential (tensiometer) is one of the irrigation scheduling methodologies. In addition, fertilization is used to promote quantity and quality of fruit production. Potassium has a key role in the osmotic adjustment of plants and alleviate the effects of drought stress. Until now, studies on citrus to evaluate the effects of potassium fertilization to mitigate the negative effects of drought stress have not been conducted. In the present study, we hypothesised that K applications via soil could contribute to osmotic adjustment of citrus and alleviate the effects of drought stress. Thus, the objective of the present study was to evaluate the effects of different soil water potential and rate of potassium (K) application on biochemical indices and growth responses of Thomson navel (Citrus, sinensis (L.) osbeck) orange seedlings on Citrumelo rootstock.
Materials and Methods: This study site was located at the Citrus and Subtropical Fruit Research Center of Horticultural Science Research Institute (36°54′11″N, 50°39′30″E), with a mean annual rainfall of 1200 mm. Thomson navel trees (Citrus, sinensis (L.) were planted at 7 × 6m distances. Soil had a pH (soil-to-water suspension ratio of 1:2) of 6.2 and contained 14.3 g kg−1 organic C and CaCO3<1%. The texture of soil was clay loam. A two-year field study was conducted in a factorial experiment based on randomized complete block design with four selected ranges of soil water potential, two levels of K application, and four replicates. Irrigations were scheduled using soil moisture tensiometers. The irrigation treatments were scheduled when soil water tensions reached 20, 40, and 60 kilopascal (kPa) on the tensiometers per treatment and results were compared with control (none irrigation) treatment. Soil water tensions of 20, 40, and 60 kPa correspond to soil water depletions of 17, 35, and 52%, respectively, of the available soil water for the studied soil. Levels of K fertilizer were 50 (k1) and 100 (K2) g K × age of tree. Potassium fertilizer was broadcast below the tree canopy in March. At the end of each year, yield, available K and some growth indices were measured. In addition, in the last year, proline, ionic leakage and electrical conductivity were also measured. All data were represented as mean of four replicates. Differences in yield, canopy diameter and available K among fertilizer and irrigation treatments and sampling years were analyzed using general linear model two-way analysis of variance (ANOVA) in SAS 9.1. Since the mentioned attributes were measured during two years to take into account annual variation, we used ANOVA procedure for a combined analysis of data. The significance of differences between the mean of treatments were determined by using Duncan test. All the statistical analyzes were performed by SAS 9.2.
Results and Discussion: The results showed that irrigation increased the canopy diameter (P<0.05). Under K1 application, the tree yield was not significantly different (P≥0.05) between irrigation at different water potentials (I1, I2 and I3). However, the K effects on tree yield depended on soil water potential and the positive effects of K2 application were evident only in the I2 and I3 treatments. However, K2 application reduced the yield in irrigation treatments including I0 and I1 significantly (P<0.05) compared with K1 application. In addition, the results of the last year showed that proline and ionic leakage were increased (P<0.05) by reduce of water potential in irrigation time. However, double application of K (K2) increased (P<0.05) proline and decreased ionic leakage as compared with normal application of K (K1). Moreover, available K and electrical conductivity were increased (P<0.05) by excessive application of K and reduce of soil water potential.
Conclusion: In summary, regarding this experiment, irrigation at 60 kPa (depletion coefficient =52%) and potassium application rate of 50 g K × age of tree was the best treatment.
 

کلیدواژه‌ها [English]

  • Citromelo
  • Ionic leakage
  • Irrigation
  • Potassium fertilizer
  • Proline
  • Tensiometer
  • yield
1- Anonymous. 2015. Agricultural Statistical. Ministry of Agricultural Jihad, Department of Planning and Economy, Center for Information and Communication Technology, Tehran, Iran. (In Persian)
2- Asadi Kangarshahi A., and Akhlaghi Amiri N. 2015. Advanced and applied citrus nutrition. Agricultural Education and Extension. (In Persian)
3- Bates L., Waldren R., and Teare I. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-207.
4- Blum A. 1996. Crop responses to drought and the interpretation of adaptation. Plant Growth Regulation, 20: 33-45.
5- Bolat M., Dikilitas S., Ercisli A.I., kinci A., and Tonkaz T. 2014. The effect of water stress on some morphological, physiological, and biochemical characteristics and bud success on apple and quince rootstocks. Scientific World Journal, 4: 769-732
6- Boman B.J., Obreza T.A., and Morgan K.T. 2008. Citrus best management practices: fertilizer rate recommendation and precision application in Florida. Proceedings of the International Society of Citriculture, 1: 573–578.
7- Bremner J.M. 1996. Nitrogen-total. p. 1085-1121. In D.L. Sparks (ed.) Methods of Soil Analysis. Part 3, chemical methods. Soil Science Society of America, Madison, Wisconsin.
8- Carlos B.L. 2013. Regulated deficit irrigation in citrus: agronomic response and water stress indicators. Ph.D. thesis, Polytechnic University of Valencia. Valencia, Spain.
9- Creighton J., Sleeper D.A., and Hubbard C. 1989. Tensiometers for irrigation scheduling in a florida citrus grove. Proceedings of the Florida State Horticultural Society, 102: 69-72.
10- Delauney A.J., and Verma D.P.S. 1993. Proline biosynthesis and osmoregulation in plants. The Plant Journal, 4: 215-223.
11- Ebadi H. 2011. Effect of water different levels of micro irrigation on quality and yield fruit of Thomson navel orange in the west of Mazandaran. Final report. Iran Citrus Research Institute. (In Persian with English abstract)
12- Fayez Kh.A., and Bazaid S.A. 2014. Improving drought and salinity tolerance in barley by application of salicylic acid and potassium nitrate. Journal of the Saudi Society of Agricultural Sciences, 13: 45-55.
13- Fifaei R., Fotouhi Ghazvini R., Golein B., and Hamidoghli Y. 2016. Effect of drought stress on proline, soluble sugars, malondialdehyde and pigments content in northern commercial Citrus rootstocks. Journal of Crops Improvement, 17: 939-952. (In Persian with English abstract)
14- Ford H. 1972. Eight years of root injury from water table fluctuations. Proceedings of the Florida State Horticultural Society, 85: 65-68.
15- Garcia A.L., Torreclllas A., Leon A., and Ruiz-Sunches M.C. 1987. biochemical Indicators of the Water Stress in Maize Seedlings. Biologia Plantarum, 29: 45-48.
16- Garcia-Sanchez F., Syvertsen J.P., Gimeno V., Botia P., and Perez-Perez J.G. 2007. Responses to flooding and drought stress by two citrus rootstock seedlings with different water-use efficiency. Physiologia Plantarum, 130: 532–42.
17- Gee G.H., and Bauder J.W. 1986. Particle size analysis. p. 383-409. In: A. Klute (ed.) Methods of Soil Analysis. Part 2, physical properties. Soil Science Society of America, Madison, Wisconsin.
18- Gimeno V., Diaz-Lopez L., Simon-Grao S., Martinez V., Martinez-Nicolas J.J., and Garcia-Sanchez F. 2014. Foliar potassium nitrate application improves the tolerance of Citrus macrophylla L. seedlings to drought conditions. Plant Physiology and Biochemistry, 83: 308-315.
19- Gonzalez-Altozano P., and Castel J.R. 1999. Regulated deficit irrigation in‘Clementina de Nules’ citrus trees. I. Yield and fruit quality effects. The Journal of Horticultural Science and Biotechnology, 74: 706–713.
20- Gonzalez-Altozano P., and Castel J.R. 2000. Regulated deficit irrigation in “Clementina de Nules” citrus trees. II. Vegetative growth The Journal of Horticultural Science and Biotechnology, 75: 388–392.
21- Helmke Ph.A., and Sparks D.L. 1996. Lithium, sodium, potassium, rubidium and cesium. p. In D.L. Sparks (ed.) Methods of Soil Analysis. Part 3, chemical methods. Soil Science Society of America, Madison, Wisconsin.
22- Hilgeman R.H., and Sharp F.O. 1970. Response of Valencia orange trees to four soils water schedules during 20 years. Journal of American Society of Horticulture Science, 95: 739 -745.
23- Hsiao T.C. 1973. Plant responses to water stress. Annual Review of Plant Biology, 24: 519-570.
24- Khammari I., Galavi M., Ghanbari A., Solouki M., and Poorchaman M.R.A. 2012. The effect of drought stress and nitrogen levels on antioxidant enzymes, proline and yield of Indian Senna (Cassia angustifolia L.). Journal of Medicinal Plants Research, 11: 2125-2130.
25- Khoshbakht D., Akbar Ramin A., and Baninasab B. 2014. Citrus Rootstocks Response to Salinity: Physio-biochemical Parameters Changes. Research Journal of Environmental Sciences, 8: 29-38.
26- Lei Y.B., Yin C.Y., and Li C.Y. 2006. Differences in some morphological, physiological, and biochemical responses to drought stress in two contrasting populations of Populus przewalskii. Physiologia Plantarum, 127: 182-191.
27- Loeppert R.H., and Sparks D.L. 1996. Carbonate and gypsum. p. 437-474. In D.L. Sparks (ed.) Methods of Soil Analysis. Part 3, chemical methods. Soil Science Society of America, Madison, Wisconsin.
28- Malik N.S.A., Perez J. L., Kunta M., Patt J.M., and Mangan R.L. 2014. Changes in free amino acids and polyamine levels in Satsuma leaves in response to Asian citrus psyllid infestation and water stress. Insect Science, 21: 707-716.
29- Mahdavi Reykande J., Akhlaghi Amiri N., and Shahabian M. 2013. Analyzing phenological stages of three citrus varieties at foothills, plain and shoreline areas of Sari in North of Iran. International Journal of Agriculture and Crop Sciences, 6: 452-457.
30- Mooreand K.J., and Dixon Ph.M. 2015. Analysis of Combined Experiments Revisited. Agronomy Journal, 107: 763-771.
31- Morgan K.T., Obreza T.A, and Wheaton T.A. 2006. Size, biomass and nitrogen relationships with sweet orange tree growth. Journal of the American Society for Horticultural Science, 131: 149-156.
32- Morgan K.T, Obreza T.A., and Scholberg J.M.S. 2007. Orange tree fibrous root length distribution in space and time. Journal of the American Society for Horticultural Science, 132: 262-269.
33- Murray T.P., and Clapp J.G. 2004. Current fertilizer salt index tables are misleading. Communications in Soil Science and plant Analysis, 35: 2867-2873.
34- Nelson D.W., and Sommers L.E. 1996. Total carbon organic carbon and organic matter. p. 961-1011. In D.L. Sparks (ed.) Methods of Soil Analysis. Part 3, chemical methods. Soil Science Society of America, Madison, Wisconsin.
35- Obreza T., and Morgan K.T. 2011. Nutrition of Florida Citrus Trees. University of Florida, IFAS Extension.
36- Olsen, S.R., and Sommers L.E. 1984. Phosphorus. p. 403-430. In A. Klute (ed.) Methods of Soil Analysis. Part1, chemical and biological properties. Soil Science Society of America, Madison, Wisconsin.
37- Paramasivam, S., Alva A.K., and Fares A. 2000. An evaluation of soil water status using tensiometers in a sandy soil profile under citrus production. Journal of Soil Science, 165: 345-353.
38- Perez-Perez J.G., Garcia J., Robles J.M., and Botia P. 2010. Economic analysis of navel orange cv. ‘Lane Late’ grown on two different drought-tolerant rootstocks under deficit irrigation in South-eastern Spain. Agricultural Water Management, 97:157-164.
39- Rhoades J.D. 1996. Salinity Electrical conductivity and total dissolved solids. p. 417-437. In D.L. Sparks (ed.) Methods of Soil Analysis. Part 3, chemical methods. Soil Science Society of America, Madison, Wisconsin.
40- Romero P., Navarro J.M., Perez-Perez J., Garcia-Sanchez F., Gomez-Gomez A., Porras I., Martinez V., and Botia P. 2006. Deficit irrigation and rootstock: their effects on water relations, vegetative development, yield, fruit quality and mineral nutrition of Clemenules mandarin. Tree Physiology, 26: 1537–1548.
41- Sanchez C.A., Wilcox M., Wright G.C., and Brown P. 1996. Efficient Irrigation and N Management for Lemons: Results for 1993-1996. Citrus Research Report, 22-44.
42- Skogley E.O., and Haby V.A. 1981. Soil Science Society of American Journal, 45:533-536.
43- Spiegel-Roy P., and Goldschmidt E.E. 1996. Biology of Citrus. Cambridge University Press.
44- Smajstrala A.G., and Koo R.C.J. 1985. Effects of trickle irrigation methods and amounts of water applied on citrus yield. Proceedings of the Florida State Horticultural Society, 97: 3-7.
45- Srivastava A.K., Shirgure P.S., and Shyam S. 2003. Differential fertigation response of Nagpur mandarin (Citrus reticulate Balanco) on an alkaline Inceptisol under sub-humid tropical climate. Tropical Agriculture, 80: 97–104.
46- Thomas G.W. 1996. Soil pH and soil acidity. p. 475-491. In D.L. Sparks (ed.) Methods of Soil Analysis. Part 3 chemical methods. Soil Science Society of America, Madison, Wisconsin.
47- Xie Sh., Liu Q., Xiong X., and Lovatt C.J. 2012. Effect of water stress on citrus photosynthetic characteristics. Acta Horticulturae, 928: 315-322
48- Yan B., Dai Q., Liu X., Huang S., and Wang Z. 1996. Flooding-induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves. Plant Soil, 179:261-268.
49- Zandalinas S.I., Rivero R.M., Martinez V., Gomez-Cadenas A., and Arbona V. 2016. Tolerance of citrus plants to the combination of high temperatures and drought is associated to the increase in transpiration modulated by a reduction in abscisic acid levels. Plant Biology, 16:1-16.
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