شبیه سازی آزادسازی روی تحت تاثیر تلقیح میکروبی و سطوح شوری در یک خاک غیر استریل آهکی با استفاده از مدل‌های سینتیکی

نوع مقاله : مقالات پژوهشی

نویسندگان

• حمید رضا بوستانی ¹
• مصطفی چرم ²
• عبدالامیر معزی ²
• نجفعلی کریمیان ³
• نعیمه عنایتی ضمیر ²
• مهدی زارعی ⁴

¹ دانشکده کشاورزی و منابع طبیعی داراب، دانشگاه شیراز

² دانشگاه شهید چمران اهواز

³ شیراز

⁴ دانشگاه شیراز

https://doi.org/10.22067/jsw.v30i3.41629

چکیده

آزادسازی روی از خاک در اثر فعالیت‌های بیولوژیکی یکی از عوامل مهم کنترل کننده قابلیت استفاده زیستی روی است. هدف از انجام این پژوهش، بررسی تاثیر تلقیح باکتری های محرک رشد و قارچ میکوریز بر سینتیک آزادسازی روی در خاک آهکی با سطوح مختلف شوری در کشت ذرت بود. آزمایشی فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در شرایط گلخانه انجام شد. فاکتور اول شامل سطوح شوری خاک (0، 15 و 30 میلی‌اکی والان نمک در کیلوگرم خاک از منابع کلرید سدیم، کلسیم و منیزیم به ترتیب به صورت ترکیبی 1:2:3) و فاکتور دوم تلقیح میکروبی (بدون تلقیح، تلقیح با قارچ، تلقیح با باکتری، تلقیح همزمان باکتری و قارچ) بود. کاربرد همه تیمارهای میکروبی میزان آزادسازی اولیه روی را نسبت به تیمار شاهد افزایش دادند. به ترتیب بیشترین و کمترین آزادسازی اولیه روی در تیمار قارچ-باکتری و باکتری مشاهده شد. همچنین با کاربرد همه تیمارهای میکروبی سرعت رهاسازی روی نسبت به تیمار شاهد کاهش یافت و کمترین کاهش در تیمار قارچ مشاهده شد. به طور کلی افزایش سطوح شوری سبب افزایش آزادسازی اولیه روی و کاهش سرعت آزادسازی روی شد. همچنین شکل محلول و تبادلی بیشترین تاثیر را در کنترل آزادسازی روی داشت. نتایج نشان داد که مدل‌های الوویچ ساده شده، سرعت دو ثابته و پخشیدگی پارابولیک توصیف خوبی را از آزادسازی روی نشان دادند. بر اساس بیشترین ضریب تبیین و کمترین خطای استاندارد، برترین مدل، الوویچ ساده شده تعیین شد، بنابراین به نظر می رسد که مکانیسم اصلی کنترل کننده آزادسازی روی، در خاک مورد آزمایش پدیده پخشیدگی باشد.

کلیدواژه‌ها

• باکتری محرک رشد گیاه
• پخشیدگی
• معادله الوویچ
• شکل محلول و تبادلی
• قارچ میکوریز

20.1001.1.20084757.1395.30.3.20.4

عنوان مقاله [English]

Simulation of Zinc Release Affected by Microbial Inoculation and Salinity Levels in a non-sterile Calcareous Soil Using kinetic Models

نویسندگان [English]

• hamidreza boostani ¹
• mostafa chorom ²
• abdolamir moezzi ²
• najafali karimian ³
• naimeh enayatizamir ²
• mehdi zarei ⁴

¹ Coledge of Agriculture and Natural Resources of Darab, Shiraz University

² Shahid Chamran University, Ahvaz

³

⁴ shiraz university

چکیده [English]

Introduction: Zinc (Zn) is an important nutrient element for humans and plants that controls many biochemical and physiological functions of living organisms. Zinc deficiency is common in high pH, low organic matter, carbonatic, saline and sodic soils. Salinity is a major abiotic environmental stresses that limits growth and production in arid and semi-arid regions of the world. Bioavailability of Zn is low in calcareous and saline soils having high levels of pH and calcium. Desorption of Zinc (Zn) from soil as influenced by biological activities is one of the important factors that control Zn bioavailability. Few reports on the effects of salinity on the availability and desorption kinetics of Zn are available. Rupa et al. (2000) reported that increasing the salt concentration led to increase Zn desorption from soil due to ion competition on soil exchangeable sites. Different kinetic equations have been used to describe the release kinetics of nutrients. Reyhanitabar and Gilkes (2010) found that the power function model was the best equation to describe the release of Zn from some calcareous soil of Iran, whereas Baranimotlagh and Gholami (2013) stated that the best model for describing Zn desorption from 15 calcareous soils of Iran was the first-order equation.less attention has been paid to kinetics of Zn release by DTPA extractant over time by inoculation of plant growth promoting rhizobacteria and mycorrhizae fungi in comination with soil salinity.The objective of this study was to evaluate the effect of plant growth promoting rhizobacteria (PGPR) and mycorrhizae fungi (MF) inoculation on release kinetic of Zn in a calcareous soil at different salinity levels after in cornplantation
Materials and Methods: A composite sample of bulk soil from the surface horizon (0-30 cm) of a calcareous soil from southern part of Iran was collected, air dried, passed through 2 mm sieve, and thoroughly mixed. Routine soil analysis was performed to determine some physical and chemical properties. The experiment was conducted in the greenhouse of agriculture college of Shahid Chamran University, Ahvaz, Iran. A factorial experiment as a completely randomized design with three replications was conducted in greenhouse conditions. The first factor consisted of salinity levels (0, 15 and 30 cmol(c) kg-1 salt supplied as a 3:2:1 Na:Ca:Mg chloride salts) and the second factor was microbial inoculation (without inoculation, fungi, bacteria, bacteria + fungi).Soil samples were extracted using DTPA extractant for periods of 0.5, 1, 2, 6, 12 and 24 hours. Cumulative Zn released (q) as a function of time (T) was evaluated using seven different kinetic models. A relatively high values of coefficient of determination (r2) and low values of standard error of estimate (SEE) were used as criteria for the selection of the best fitted models. Statistical analysis of data was done using MSTATC package (Mstatc, 1991). Comparison between means was performed using Duncan's multiple range test (DMRT) at the significant level of P < 0.05. Also, charts were drawn by excel computer package.
Results and Discussion: Investigation of Zn release patterns showed that the control and all treated soils had a uniform pattern of Zn release. Overall, Zn release patterns were generally characterized by an initial fast reaction at first two hours, followed by slower continuing reaction. It seems likely that the release of zinc is controlled by two different mechanisms. Two-step process of releases (rapid and subsequent slow) is attributed to the existence of places with different energy. The use of all microbial treatments increased the initial release of Zn compared to control. The most and the least Zn initial release observed in fungi-bacterial and bacterial treatment respectively. By application of all microbial treatments, Zn release rate declined compared to control and the lowest decrease observed in fungal treatment. In general, Zn initial release was increased and Zn desorption rate was decreased by increasing of salinity levels. Also, soluble and exchangeable forms of Zn had the highest influence on Zn release control.
Conclusions: Results showed that simplified Elovich, two constant rate and parabolic diffusion kinetics models showed good description of the Zn release. Based on the highest correlation coefficient and the lowest mean standard error of the estimate, simplified elovich determined as the best kinetic model. So it seems that the main mechanism controlling the Zn release in the tested soil is diffusion phenomena.

کلیدواژه‌ها [English]

• Diffusion
• Elovich Equation
• Mycorrhizae Fungi
• PGPR
• Water and Soluble Form

مراجع

Aharoni C., Sparks D.L., Levinson S., and Revina I. 1991. Kinetics of soil chemical reactions: Relationship between empirical equations and diffusion models. Soil Science Society of American Journal, 55: 1307-1312.

2-Alloway B.J. 2004. Zinc in soils and crop nutrition. International Zinc Association (IZA). http://www.Zinc-crop.org, pp: 128

3-Alloway B.J. 2009. Soil factors associated with zincdeficiency in crops and humans. Environment and Geochemical Health, 31: 537–548.

4-Banai M.G., Moemeni A., Baybordi M., and Malakouti M.G. 2004. Iranian soil, new developments in the diagnosis and management. Sana publication, Tehran, Iran. (In Persian)

5-Baranimotlagh M., and Gholami M. 2013. Time_dependent zinc desorption in some calcareous soils of Iran. Pedosphere, 23(2): 185-193.

6-Bedrossian S., and Singh B. 2004. Kinetics of potassium release from vertisoils from northern NSW. In: Super Soil : Australian New Zealand Soils Conference,December 2004. Sydney, University of Sydney, Australia.

7-Bremner J.M. 1996. Nitrogen- Total. P. 1085- 1122. In: D. L. Sparks. et al., (eds) Methods of Soil Analysis. Part III. 3rd ed, Am. Soc. Argon., Madison, WH.

8-Chien S., and Clayton W.R. 1980. Application of Elovich equation to the kinetics of phosphate release and sorption in soils. Soil Science Society of American Journal, 44:265-286.

9-Dodd J.C., Burton C.C., and Jeffries P. 1987. Phosphatase activity associated with the roots and the rhizo‌sphere of plants infected with vesicular arbuscu‌lar mycorrhizal fungi. New Physiology, 107: 163-172.

10-Dang Y.P., Dalal R.C., Edwards D.G., and Tiller K.G. 1994. Kinetics of zinc desorption from Vertisols. Soil Science Society of American Journal, 58: 1392–1399.

11-Gee G.W., and Bauder J.W. 1986. Particle size analysis, hydrometer method. P. 404-408. In A. Klute et al(eds). Methods of soil analysis. 2 nd ed. Part 1.America Society of Agronomy, Madison. WI.

12-Ghasemi Fasaei R., Tavajjoh M., Oloma V., Molazem B., Maftoun M., Ronaghi A., Karimian N., and Adhami E. 2007. Copper release characteristics in selected soils from southern and northern Iran. Australian Journal of Soil Research, 45: 459-464.

13-Ghasemi-phasaei R., Maftoun N., Olama V., Molazem B. and Tavajjoh M. 2009. Manganese release characteristics of highly calcareous soils. Communication in Soil Science and Plant Anal., 40(7-8): 1171-1182.

14-Gholami A., Shahsavandi S., and Nezarat S. 2009. The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maze. Proceeding of World Academy of Science. Engineering and Technology, 37: 2070-3740.

15-Gholami M., Baranimotlagh M., Dardipour A., and Olamai M. 2011. Simulation of Zinc releases kinetics from different soils of Golestan province using DTPA extractant. Soil and Water Protection Research, 18(2):123-139. (in Persian with English abstract)

16-Habashy N.R., and Abo-Zied M.M.A. 2005. Impact of Cd-Pb polutted water on growth and elemental compo‌sition of onion plants growth on a calcareous soil inoculated with mycorrhiza. Egyptian Journal of Applied Science, 20: 586-594.

17-Havlin J.L., Westfall D.G., and Olsen S.R. 1985. Mathematical models for potassium release kinetics in calcareous soils. Soil Science Society of American Journal, 49: 371–376.

18-Helmke P.H., and Spark D.L. 1996. Potassium. p: 551-574. In Sparks D.L., et al. Methods of Soil Analysis.SSA,Inc. ASA, Inc. Madison, WI

19-Kamali, S., Ronaghi, A., and Karimian, N. 2011. Soil Zinc Transformations as Affectedby Applied Zinc and Organic Materials. Communication in Soil Science and Plant Analysis, 42(9): 1038-1049

20-Kandpal G., Srivastava P.C., and Ram B. 2005. Kinetics of desorption of heavy metals from polluted soils: influence of soil type and metal source. Water Air Soil Pollution, 161: 353–363.

21-Kim T.W., and Lei X.G. 2005. An improved method for a rapid determination of phytase activity in animal feed. Journal of Animal Science, 83: 1062–1067

22-Lehmann R.G., and Harter R.D. 1984. Assessment of copper-soil bond strength by desorption kinetics. Soil Science Society of American Journal, 48: 769-772.

23-Lindsay W.L., and Norvell W.A. 1978. Development of DTPA soil test for zinc, iron, manganese and copper. Soil Science Society of American Journal,42: 421-428.

24-Lindsay W.L., and Cox F.R. 1985. Micronutrient soil testing for the tropics. Fertilizer Research 7, 169–200.

25-Loeppert R.H., and Suarez D.L. 1996. Carbonate and Gypsum. p: 437-474. In Sparks D.L., et al. Methods of SoilAnalysis. SSSA, Inc. ASA, Inc. Madison, WI.

26-Maftoun M., and Karimian N. 1989. Relative efficiency of two zinc sources for maize (Zea mays L.) in two calcareous soils from an arid area of Iran. Agronomy, 9: 771–775.

27-Martin H.W., and Sparks D.L. 1983. Kinetics of nonexchangeable potassium release from two coastal plain soils. Soil Science Society of American Journal, 47: 883–887.

28-Mengel K., Rahmtullah H. and Dou H. 1998. Release of potassium from the silt and sand fraction of loess-drived soils. Soil Science, 163: 805-813.

29-Mehboob I., Naveed M., and Zahir Z.A. 2009. Rhizobial Association with Non-Legumes: Mechanisms and Applications. Critical Reviews in Plant Science, 28: 432–456.

30-Motaghian H.R. and Hosseinpour A. 2013. Characteristics Change of copper release in wheat rhizosphere of some calcareous soils. Soil and Water Research, 44(1): 107-122. (in Persian with English abstract)

31-Nelson D.W. and Sommers L.E. 1996. Total carbon, organic carbon and organic matter. P. In D. L. Sparks et al., (eds). Methods of soil analaysis. Part III. 3 rd Ed., Am. Soc. Agron., Madison, WI.

32-Olsen S.R., Cole C.V., Watanabe F.S and Dean L.A. 1954. Estimation of available phosphorous in soil by extraction with sodium bicarbonate. USDA. Cric. 939. U. S. Gov. Print. Office, Washington, DC.

33-Orhan E., Estiken A., Ercisli S., Taran M., and Sahin, F. 2006. Effect of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Scienta Horticulture, 11: 38-43.

34-Page A.L., Changeand A.C., and Adriano D.C. 1996. Deficiencies and Toxicities of Trace Elements. In: “Agricultural salinity assessment and management” No. 71 (Ed.) Tanji, K. K., pp. 138-160. Amer. Soc. Civil Eng., New York

35-Poletti S., Gruissen W., and Sautter C. 2004. The nutri‌tional fortification of cereals. Current Opinion Biotechnology, 15: 162–165

36-Qadir M., Qureshi R.H., and Ahmed N. 1997. Nutrient Availability in a Calcareous Saline-sodic Soil during Vegetative Bioremediation. Arid Soil Research, 11: 343-352.

37-Rattan R.K., and Sharma P.D. 2004. Main micronutrients available and their method of use. Proceedings IFA International Symposium on Micronutrients, 1-10.

38-Ravikovitch S., Margolin M., and Navrot J. 1968. Zinc Availability in Calcareous Soils: I. Comparison of Chemical Extraction Methods for Estimation of Plant Availability Zinc. Soil Science, 105: 57-61.

39-Reyhanitabar A., and Karimian N. 2008. Kinetics of copper desorption of selected calcareous soils from Iran. American-Eurasian Journal of Agricultural and Environmental Science, 4(3): 287-293.

40-Reyhanitabar A., and Gilkes R.J. 2010. Kinetics of DTPA extraction of zinc from calcareous soils. Geoderma, 154: 289-293.

41-Reyhanitabar A., Ardalan M., Gilkes R.J. and Savaghebi J. 2010. Zinc sorption characteristics of some selected calcareous soils of Iran. Journal of Agricultural Science and Technology, 12: 99-110.

42-Rupa T.R., Tomar K.P., Damidar Reddy, D., and Subba Rao, A. 2000. Time-dependent zinc desorption in soils. Communication in Soil Science and Plant Analaysis, 31: 2547-2563.

43-Sinha M.K., Dhillon S.K., and Dhillon K.S. 1977. Zinc chelate reaction in alkaline soils. Australian Journal of Soil Research, 15: 103–113.

44-Singh, J.P., Karwarsa, S.P.S., and Singh, M. 1988. Distribution and forms of copper, iron, manganese, and zinc in calcareous soils of India. Soil Science, 146: 359-366.

45-Sposito, G., Lund, L.J., and Chang, A.C. 1982. Trace metal chemistry in arid zone field soils amended with sewage sludge: I. Fractionation of Ni, Cd and Pb solid phases. Soil Science Society of American Journal,46: 260-264.

46-Subramanian K.S., Bharathi C., and Jegan R.A. 2008. Re‌sponse of maize to mycorrhizal colonization at varying levels of zinc and phosphorus. Biology and Fertility of soil, 8: 317-328

47-Subramanian K.S., Charest C., Dwyer L.M., and Hamilton R.I. 1997. Effects of mycorrhizas on leaf water poten-tial, sugar and P contents during and after recov‌ery of maize. Canadian Journal of Botany, 75: 1582-1591.

48-Strawn D.G., and Sparks D.L. 2000. Effect of soil organic matter on the kinetics and mechanisms of Pb(II) sorption and desorption in soil. Soil Science Society of American Journal, 64:144-156.

49-Sparks D.L. 1989. Kinetics of soil chemical processes. Academic Press. San Diego. California. USA.

50-Steel R.G.D., and Torrie J.H. 1960. Principles and procedures of statistics. McGraw-Hill, New York.

51-Thomas G.W. 1996. Soil and Soil acidity. Pp:475-490. In: D. L. Sparks et al., (eds). Methods of Soil Analysis. Part III. 3rd Ed., Am. Soc. Agron., Madison, WI.

52-Uygur V., and Rimmer D.L. 2000. Reaction of zinc with iron coated calcite surface at alkaline pH. Europ. Journal of Soil Science, 51, 511–516.

53-Wamberg C., Christensen S.I., Jakobsen A.K., Muller I., and Sorensen S.J. 2003. The mycorrhizal fungus (Glo‌mus intraradices) affects microbial activity in the rhizosphere of pea plants (Pisum sativum). Soil Biology and Biochemistry, 35: 1349–1357

54-Wright S. F., and Upadhyaya A. 1998. A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant Soil 198: 97-107.

55-Yildirim E., Turan M., and Donmez M.F. 2008. Mitigation of salt stress in radish (RaphanusSativus l.) by plant growth promoting rhizobacteria. Roumanian Society of Biological Science, 13 (5): 3933-3943.

56-Zahedifar, M., Karimian, N., and Yasrebi, J. 2012. Influence of applied zinc and organic matter on zinc desorption kinetics in calcareous soils, Archive of Agronmy and Soil Science, 58:2, 169-178.