Document Type : Research Article

Authors

Vali-e-Asr University of Rafsanjan

Abstract

Introduction: Pistachio is one of the most important crops in many regions of Iran with respect of production and export. There are more than 470000 ha of nonbearing and bearing pistachio trees mainly in Kerman province. Despite the economic importance of this crop, very little information is available on its nutritional requirements. Pistachio trees like other crops need to macro and micro nutrients. one of these elements is manganese (Mn). Manganese is an essential mineral nutrient, playing a key role in several physiological processes, particularly photosynthesis, respiration and nitrogen assimilation. This element is normally supplied to the plants by soil. Therefore, soil conditions affect its availability to plants. Soils with high pH, calcareous soils, especially those with poor drainage and high organic matter, are among the soils which produce Mn-deficient plants. Calcium carbonate is the major inactivation factor of Mn in calcareous soils. The soils of Iran are predominantly calcareous in which micronutrients deficiency, including Mn, is observed due to the high pH and nutrient fixation. The objective of this research was to examine the effect of manganese application on growth and chemical composition of pistachio seedlings in some calcareous soils with different chemical and physical properties.
Materials and Methods: For this purpose a greenhouse experiment was carried out as factorial (two factors including soil type and Mn levels) experiment in completely randomized design with three replications. Treatments were consisted of three levels of Mn (0, 10 and 20 mg Mn Kg-1 soil as Manganese sulfate) and 12 different soils from Rafsanjan region in Southern Iran. Soil samples were air dried and crushed to pass through a 2-mm sieve, and some physical and chemical properties of soils such as texture, electrical conductivity, pH, organic matter content, calcium carbonate equivalent, cation exchange capacity and iron, manganese, copper and zinc availability were determined. Then plastic pots were filled with 5 kg of these soils. Pistachio seeds (cv Badami Zarand) were placed in muslin sacks and pretreated for 24 h with Benomyl solution. The germinated seeds were planted in each pot, and each pot was irrigated with distilled water. Nitrogen and phosphorous were applied uniformly to all pots at the rate of 50 mg kg−1 soil as ammonium nitrate and potassium dihydrogen phosphate forms, respectively. Zinc, iron and copper also were added to treatments at level of 5 mg kg−1 soil as zinc sulfate, iron sequestrine138 and copper sulfate. After 24 weeks, the seedlings were cut at the soil surface, and the roots were washed free of soil. Leaves, stems and roots were dried at 70 oC for 48 h in an oven. The total leaf, stem, and root dry weights were recorded. The ground plant samples were dry- ashed at 550oC, dissolved in 2 N HCl, and made to volume with hot distilled water. Plant Mn, Cu, Zn and Fe concentrations determined by atomic absorption spectrophotometry. All data were statistically analyzed according to the technique of analysis variance (ANOVA) by MSTATC.
Results and Discussion: Results indicated that the application of manganese increased leaf, stem and root dry weight of pistachio seedlings so that the maximum amount of the dry weight of roots stems and leaves of pistachio seedlings were observed at 10 mg Mn kg-1soil. Application of 10 mg Mn kg-1 soil increased leaf, stem and root dry weight by 19.2%, 25.2% and 23.9% in comparison to control, respectively. Chemical composition (concentration and uptake) of shoot of pistachio seedlings was also affected by Mn application. Mn application decreased the concentration and uptake of iron, concentrations of copper and zinc in Pistachio seedling shoots so that the highest concentrations of these elements were observed in control treatment. Reductions in concentrations of zinc and copper elements in 10 mg Mn kg-1 treatment were not statistically significant but in 20 mg Mn kg-1 treatment they were significant.
Conclusions: The results of this research showed that the application of 10 mg Mn kg-1soilto highly calcareous soils significantly increased most of growth parameters of pistachio seedlings in comparison to control. Although higher amounts of manganese (20 mg Mn kg-1 soil) reduced the growth parameters than the previous level. In overall, the results of this study indicated that for optimum growth and chemical composition of pistachio seedlings in calcareous soils, application of 10 mg Mn kg-1 sol is necessary.

Keywords

1- Afrousheh M., Hokmabadi H., and Hosseini H.M. 2010. Effect of nitrogen, iron, magnesium, manganese and molybdenum deficiencies on biochemical and ecophysiological characteristics of pistachio seedling (Pistaciavera), Ciheam Options Mediterraneannes, 94:53-63.
2- Alam S.M., and Ansari R. 2001. Influence of iron and manganese on the growth and contents of Fe, Mn and P in rice. Journal of Biological Sciences, 1(6):434-435.
3-Alipour H., and Hosseinifard J. 2003. Detection and alleviation nutrient deficiency in pistachio. Ministry of Agriculture, Agricultural Research Organization, Page 53.
4- Alison L.E., and Moodie C.D. 1965. Carbonate. In: C. A. Black et al. (eds.), Methods of soil analysis. Part II, American Society of Agronomy, Madison, WI, 1379-1396.
5- ArefF. 2011. Influence of zinc and boron nutrition on copper, manganese and iron concentration in the maize leaf. Australian Journal of Basic and Applied Sciences, 5(7):52-62.
6- AsadiKangarshahi A., AkhlaghiAmiri N., and Malakouti M.J. 2011. Residual effects of manganese sulfate on growth, yield and manganese uptake in soybean. Iranian Journal of Soil and Water Research, 1(42):143-153. (in Persian with English abstract).
7- Bouyoucos G.J. 1951. A recalibration of hydrometer method for making mechanical analysis of soil. Agronomy Journal, 43:434-438.
8- Burnell J.N. 1988. The biochemistry of manganese in plants.In Manganese in soils and plants. pp. 125-137. Kluwer Academic Publishers, Dordrecht, the Netherlands.
9- Chapman H.D. 1965. Cation exchange capacity. In: C. A. Black et al. (eds.), Methods of soil analysis. Part II, American Society Agronomy, Madison, WI, USA, 891-900.
10- Dominy A., and Bertling I. 2001. Manganese, zinc and silicon studies of cucumber (Cucumissativus) using a miniature hydroponic system. ActaHorticulturae (ISHS), 644:393-398.
11- El-Shazly S.M., and Dris R. 2004. Response of'Anna'apple trees to foliar sprays of chelated iron, manganese and zinc. Journal of Food, Agriculture and Environment, 2(3-4):126-130.
12-Ghafarinejad S.A., and Karimian N.A. 1998.The correlation between manganese extracted by five procedure with soil properties and soybean responsein calcareous soils of Fars state. Journal of Science and Technology of Agriculture and Natural Resources, 2(4):65-76. (in Persian).
13- GholamalizadehAhangar A., Karimian N., Abtahi A., Assad M.T., and Emam, Y. 1995. Growth and manganese uptake by soybean in highly calcareous soils as affected by native and applied manganese and predicted by nine different extractants. Communications in Soil Science and Plant Analysis, 26(9-10):1441-1454.
14- Haldar M., and Mandal L.N. 1982.Cu x Mn interaction and the availability of Zn, Cu, Fe, Mn and P in waterlogged rice soils. Plant and Soil, 69(1):131-134.
15- Hasani M., Zamani Z., Savaghebi, G., and Fatahi R. 2012. Effects of zinc and manganese as foliar spray on pomegranate yield, fruit quality and leaf minerals. Journal of Soil Science and Plant Nutrition, 12(3):471-480.
16- Hatam Z., and Ronaghi A. 2011.Growth and nutrient simbalance in silage corn as affected by copper and manganese application in a calcareous soil. Iranian Journal of Soil Research (Formerly Soil and Water Sciences), 25(3):197-206. (in Persian with English abstract).
17- Jackson M.L. 1975. Soil chemical analysis, advanced course.University of Wisconsin College of Agriculture Department of Soil Science, Madison, WI., U.S.A.
18- Karimian N., and GholamalizadehAhangar A. 1998. Manganese retention by selected calcareous soil as related to soil properties. Communications in Soil Science and Plant Analysis, 29:1061-1070.
19- Karimian N., and Hashemi S.M. 2002. Manganese nutrition of wheat as affected by phosphorus and manganese application to a calcareous soil. Journal of Plant Nutrition, 92:834-835.
20- Khorsandi F., AlaeiYazdi F., and Vazifehshenas M.R. 2009. Foliar zinc fertilization improves marketable fruit yield and quality attributes of pomegranate. International Journal of Agriculture and Biology, 11 (6):766-770.
21- Kosesakal T., and Unal M. 2010. Role of zinc deficiency in photosynthetic pigments and peroxidase activity of tomato seedlings. IUFS Journal of Biology, 68(2):113-120.
22- Kuo S., and Mikkelsen D.S. 1981. Effect of P and Mn on growth response and uptake of Fe, Mn and P by sorghum. Plant and Soil, 62(1):15-22.
23- Lidon F.C. 2002. Micronutrient uptake and translocation in Mn treated rice. Journal of Plant Nutrition, 25(4):757-768.
24- Lindsay W.L., and Norvell W.A. 1978. Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Science Society of America Journal, 42:421-428.
25-Malakouti M.J., and Tehrani M.M. 2008. The role of micronutrients to increase yield and improve the quality of agricultural products (micro elements with macro effect). TarbiatModarres University, Tehran.
26-Malakouti M.J., Keshavarz P., and Karimian N.A. 2008. Methods of diagnosis and recommend the best fertilizer for sustainable agriculture. TarbiatModarres University, Tehran.
27- Marschner H. 1995. Mineral nutrition of higher plants, 2nd ed., Academic Press, London, UK, pp. 324-333.
28-Mirzaeekhalilabadi H.R., and Chizari A.H. 2004. Determination of technical efficiency and optimum amount of water in pistachio production. Journal of Research and reconstruction in agriculture and horticulture, 49:43-62.(in Persian).
29- Mousavi S.R., Galavi M., and Ahmadvand G. 2007. Effect of zinc and manganese foliar application on yield, quality and enrichment on potato (Solanumtuberosum L.). Asian Journal of Plant Sciences, 6(8):1256-1260.
30- Nielsen D., Nielsen, G.H., Sinclair, A.H., and Linehan, D.J. 1992. Soil phosphorous status, pH, and manganese nutrition of wheat. Plant and Soil, 145:45-50.
31- Olsen S.R., Cole C.V., Watanabe, F.S., and Dean, L.A. 1954. Estimation of available phosphorous in soil by extraction with sodium bicarbonate. USDA Circ. 939, U.S. Govern. Print. Office, Washington, D. C., U.S.A.
32- Papadakis I.E., Sotiropoulos T.E., and Therios I.N. 2007. Mobility of iron and manganese within two citrus genotypes after foliar applications of iron sulfate and manganese sulfate. Journal of Plant Nutrition, 30:1385-1396.
33- Richards L.A. 1954. Diagnosis and improvement of saline and alkali soils. U.S.D.A. Handbook, 60. Washington, D.C., U.S.A.
34- Salardini A.A. 1987. Soil Fertility. Tehran University Publications.
35- Sarcheshmehpoor M. 1998. Investigation of the interactions between zinc and iron, copper and manganese in pistachio seedlings, MSc Thesis in Soil Science, College of Agriculture, Tehran University, Tehran, Iran.
36- Shibli R.A., Shatnawi M.A., Mohammad M.J., Hindiyeh M.Y., and Abu-Ein A. 2007. Influence of Zn and Mn levels on growth and micronutrient acquisition of apple microculture. American-Euroasian Journal of Agricultural and Environmental Science, 2(2):147-152.
37- Tanq M., Sharif M., Shah Z., and Khan R. 2007. Effect of foliar application of micronutrients on the yield and quality of sweet orange (Citrus sinensis L.). Pakistan Journal of Biological Sciences, 10(11):1823-1828.
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