سیدسجاد حسینی امیر لکزیان اکرم حلاج نیا


افزودن EDTA به عنوان یک عامل کلات کننده به خاک‌های آلوده به فلزات سنگین ممکن است فراهمی و استخراج گیاهی فلزات را از خاک افزایش دهد اما می‌تواند اثرات منفی بر ویژگی‌های بیولوژیکی خاک داشته باشد. به منظور بررسی اثر کاربرد EDTA و اسید سیتریک بر فراهمی سرب، فعالیت دهیدروژناز، اوره‌آز و فسفومونواستراز قلیایی و همچنین تنفس میکروبی برانگیخته (SIR)، آزمایشی در قالب طرح کاملاً تصادفی با آرایش فاکتوریل و سه تکرار در شرایط گلخانه اجرا گردید. تیمارهای آزمایش شامل EDTAو اسید سیتریک در دو سطح 3 و 5 میلی مول بر کیلوگرم به همراه شاهد و زمان (7، 14، 21 و 28 روز) بودند. نتایج نشان داد که به طور میانگین تیمارهای EDTA3 و EDTA5 به ترتیب سبب افزایش 17/2 % و 10 % غلظت فراهم سرب نسبت به شاهد شدند. این در حالی است که بررسی اثر زمان نشان داد که فراهمی سرب برای تیمار EDTA3 در زمان 28 در مقایسه با اولین زمان نمونه برداری (روز هفتم) 3/12 % کاهش یافت در حالی که برای تیمار EDTA5 تغییر معنی‌داری در طی زمان مشاهده نشد. همچنین به طور میانگین تیمارهای CA3 و CA5 به ترتیب سبب کاهش 3/8 % و 7/15 % غلظت فراهم سرب نسبت به شاهد شدند به طوری که در آخرین زمان نمونه برداری فراهمی سرب به ترتیب 9/15 % و 8/12 % نسبت به اولین زمان کاهش پیدا کرد. نتایج نشان دهنده اثر منفی EDTA بر فعالیت آنزیم‌های دهیدروژناز و اوره‌آز خاک بود. از طرف دیگر افزودن اسیدسیتریک اثر مثبتی بر فعالیت آنزیم‌های دهیدروژناز، اوره‌آز و فسفومونواستراز قلیایی خاک داشت. در تمام تیمارها به جز تیمار شاهد فعالیت آنزیم‌های مورد مطالعه با گذشت زمان افزایش پیدا کرد که این افزایش در تیمارهای اسید سیتریک بیشتر بود. افزودن EDTA و اسیدسیتریک مقدار SIR خاک را در همه زمان‌های مورد مطالعه افزایش دادند که این افزایش برای تیمارهای اسیدسیتریک بیشتر از EDTA بود.

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ارجاع به مقاله
حسینیس., لکزیانا., & حلاج نیاا. (2016). اثر کاربرد EDTA و اسیدسیتریک بر تغییرات فعالیت آنزیمی خاک، تنفس میکروبی برانگیخته و فراهمی سرب در خاک. آب و خاک, 30(6), 2032-2045. https://doi.org/10.22067/jsw.v30i6.50232
نوع مقاله
علمی - پژوهشی