Study of Sage (Salvia officinalis L.) Cultivation in Condition of Using Irrigated Water Polluted By Cadmium and Lead

Document Type : Research Article

Authors

• Sh. Amirmoradi
• P. Rezvani Moghaddam
• A. Koocheki
• Shahnaz Danesh
• A. Fotovat

Ferdowsi University of Mashhad

https://doi.org/10.22067/jsw.v29i5.51508

Abstract

Introduction: Accumulation of heavy metals in agronomic soils continuously by contaminated waste waters not only causes to contamination of soils but also it affects food quality and security. Cadmium and lead are one of the most important heavy metals due to long permanence and persistence in soil can cause problems to human and animal health. Some medicinal plants are able to accumulate of heavy metals from contaminated soils. Heavy metals are not able to enter in the essential oil of some aromatic plants. Study of these plants helps human to select them for cultivating the resistant medicinal plants in contaminated soils.
Materials and Methods: This experiment was carried out in the research greenhouse of agriculture faculty of Ferdowsi university of Mashhad in 2011. Seeds were cultivated in planting aprons into peat moss medium. Then the uniform plantlets were transferred into soil in the plastic boxes (30×50×35 cm) at two leaf stage. In each box 6 plantlets were sown with distance of 15 cm on the planting rows and 20 cm between rows. Experiment was set up as factorial on the basis of randomized complete block design with three replications. The first factor was cadmium concentrations consisted of 0,10,20,40 mg per kilogram and the second factor was lead concentrations consisted of 0,100,300 and 600 mg/kg. Plants were irrigated during of15 weeks with cadmium and lead nitrogen nitrate solutions and then irrigated with distilled water. The differences of nitrogen amounts in treatments were compensated with ammonium nitrate on the basis of differences between level of the highest treatment and the treatment which obtained lower amount of nitrogen. Plants were harvested after 180 days at the beginning of flowering. All shoots and roots were weighted separately as fresh weight and then were dried under shading and then were weighted. The essential oil sage was determined by using of 30 grams of dried sage leaves with distillation method with Clevenger. Cadmium and lead contents in shoot and root were measured by wet digestion method (digestion by Perchloric and Nitric acid). Cadmium and lead contents were detected by atomic absorption apparartus. Data were analyzed by MSTATC software and all means were compared by DMRT at 5% of probability.
Result and Discussion: Results argued that fresh weight of sage at 40 mg/kg of cadmium were decreased 4.61% as compare as control. Dry weight of sage decreased at 600 mg/kg of lead 11.08 % as compare of control. Mean comparisons indicated that at the highest concentrations of cadmium and lead fresh and dry weight of sage were dropped. Growth decrement due to toxicity of cadmium causes to photosynthesis and respiration decline, carbohydrate metabolism decreasing and leaf chlorosis. Researchers observed lead ions by interfering with water balance lead to water stress. High concentrations of lead may cause to decrease the availability of water for plant and high concentrations of cadmium causes to disturb the protein synthesis and lead to protein decline in plant cells. Plant height of sage was declined at 40 mg/kg and 600 mg/kg as compared as control 14.17 and 10.83, respectively. Essential oil in sage was dropped in high levels of cadmium and lead as compare of control 12 and 14.51, respectively. Researchers stated that cadmium concentrations of 2,6 and 10 mg/lit and 50,100 and 500 mg/kg of lead had no significant effect on peppermint, but caused to drop the essential oil percentage of dill and basil.
Disturbance of carbon nutrition in plant cells during the photosynthesis process by heavy metals lead to a decrease in the essential content. The most cadmium absorption by sage shoots belonged to 40 mg/kg and 600 mg/kg of cadmium and lead, respectively and then 40 mg/kg cadmium and 300 mg/kg lead were ranked as second treatment. Increase of cadmium and lead concentrations in irrigation water led to increase of these heavy metals into sage shoots. Increase of lead and cadmium concentrations caused to antagonistic effects of cadmium and lead absorption into shoots of sage. In this experiment cadmium and lead concentrations of all treatments were too below to detect by atomic absorption apparatus. In this study cadmium and lead could not enter to essential oil. Researchers stated that high doses of cadmium, lead, zinc and copper concentrations could not enter into essential oil in sage. Some researchers showed that cadmium, lead and copper were not transferred to essential oil of peppermint, dill and basil during the essential oil distillation process. This finding confirmed that selection of medicinal plants as alternative plants with crops in cadmium and lead contaminated soils.
Conclusion: Fresh and dry weight of Sage in the condition of contaminated soil by 100 mg/kg cadmium and 600 mg/kg lead were declined 4.61 and 5.16 % as compare as control, respectively. At the highest doses of cadmium and lead the essential oil of sage were dropped but, these heavy metals were not detected in essential oil. So, it is seems that this medicinal plant may be applied in the contaminated soil or in the condition of using of contaminated irrigated water by cadmium and lead.

Keywords

• Medicinal plant
• Heavy metals uptake
• morphological traits
• Essential oil content

20.1001.1.20084757.1394.29.5.25.6

References

1-Ahmad Kamel H. 2008. Lead Accumulation and its effect on photosynthesis and free amino acids in Vicia faba grown hydroponically. Australian Journal of Basic and Application Science. 2(3): 438-446.

2-Angelova V. K., Ivanov and Ivanova R. 2004. Effects of chemical forms of lead, cadmium, and zinc in polluted soils on their uptake by tobacco. J. Plant Nutrition. 27(5): 757-773.

3-Aycicek M., Ince M., and Yaman. M. 2008. Effects of cadmium on the germination, early seedling growth and metal content of cotton (Gossypium hirsutum L.). International Journal of Science and Technology. 3(1):1-11.

4-Balestrasse K.B., Benavides M.P., Gallego S.M., and Tomaro, M.L. 2003. Effect of cadmium stress on nitrogen metabolism in nodules and roots of soybean plants. Functional Plant Biology. 30: 57-64.

5-Bavi Kh., Kholdebarin B., and Moradshahi. A. 2011. Effect of cadmium on growth, protein content and peroxidase activity in pea plants. Pakistan. J. Bot. 43(3): 1467-1470.

6- Baudouin C., Charveron M., Tarrouse R., and Gall Y. 2002 Environmental pollutants and skin cancer. Cell Biology and Toxicology. 18:341-348.

7-Begonia G. B. 1997. Comparative lead uptake and response of some plants grown on lead contaminated soils. J. Missisipi Academy Science. 42(2): 101-106.

8-Brennan M.A., and Shelley M.L. 1999. A model of the uptake, translocation, and accumulation of lead (Pb) by maize for the purpose of phytoextraction. Ecology and Engineering. 12: 271.

9-Croteau R., Burbott A.J., and Lommis W.D. 2006. Biosynthesis of mono and sesquiterpenes in peppermint. Phytochemistry. 71: 2937-2948.

10-Croteau R. and Johnson M.A. 1984. Biosynthesis of terpenoids in glandular trichomes. Chemistry of Plant Trichomes. Pp:133-185.

11-Curtis M. 2000. Healing Plants. Prague: Aventium.

12-Chen Y., Wang C., and Wang Z. 2005. Residues and source identification of persistent organic pollutants in farmland soils irrigated by effluents from biological treatment plants. Environment International 31: 778–783.

13-Chen Y.X., He Y.F., Luo Y.M., Yu Y.L., Lin Q., and Wong M.H. 2003. Physiological mechanism of plant roots exposed to cadmium. Chemosphere. 50: 789-793.

14-Czepak M. P. 1998. Produção de oleo bruto e mentol cristalizavel em oito freqüências de colheita da menta (Menta arvensis L.). Sciences, Botucatu: 53-80.

15-Cunningham L.M., Collins F.W., and Hutchinson T.C.1975. Physiological and biochemical aspects of cadmium toxicity in soybean. In: Proceed of the Int. Conf. Heavy Metals in the Environment, Toronto, October 27:97–103.

16-Clemens S. 2006. Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie. 88:1707-1719.

17-Ghadreian S.M., and Jamali Hajiani N. 2010. Tolerance, uptake and accumulation of cadmium in Matthiola chenopodiifolia Fisch & C. A. Mey (Brassicaceae). Journal of Plant Biology. 6:87-98.

18-Ghani A. 2010. Effect of Cadmium Toxicity on the Growth and Yield Components of Mungbean [Vigna radiata (L.) Wilczek]. World Applied Sciences. (Special Issue of Biotech. Genetic Engin) 8: 26-29.

19-Hall J.L. 2002. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany. 53: 1–11.

20-Harmens H., Hartog P.R.D., Bookum W.M.T., and Verkleij J.A.C.1993. Increased zinc tolerance in Silene vulgaris (Moench) Garkle is not due to increased production of phytochelatins. Plant Physiol. 103:1305-1309.

21-Hassan M. J., Zhu Z., Ahmad B. and Mahmood F. 2006. Influence of cadmium toxicity on rice genotypes as affected by zinc, sulfur and nitrogen fertilizers. Caspian Journal of Environmental Science. 4(1): 1-8.

22-Hayat Sh., Hasan S.A., and Ahmad A. 2011. Growth, nitrate reductase activity and antioxidant system in cadmium stressed tomato (Lycopersicon esculentum Mill.) cultivars. Biotechnology and Agronomy Society and Environment. 15(3):401-414.

23-Heidari M., and Sarani S. 2011. Effect of lead and cadmium in seed germination, seedling growth and antioxidant enzymes activities of Mustard (Sinapis arvensis L.). Journal of Agriculture and Biological Science. 6(1) :44-47.

24-Hernandez L.E., Carpena-Ruiz R., and Garate A. 1996. Alteration mineral nutrition of pea seedlings exposed to cadmium. Journal of Plant Nutrition. 19: 1581-1598.

25-Johna R., Ahmadb P., Gadgil K., and Sharma S. 2009. Heavy metal toxicity: Effect on plant growth, biochemical parameters and metal accumulation by Brassica juncea L. International Journal of Plant Production. 3:3.

26-Jun R., Ling T., and Guanghua Z. 2009. Effects of chromium on seed gemination, root elongation and coleoptile growth in six pulses. International Journal of Environmental Science Technology. 6: 571-578.

27-Kabata-Pendias A., and Pendias H.1991. Trace Elements in Soils and Plants, second ed. CRC Press. 365.

28-Kabir M., Zafar Iqbal M., Shafiq Z.R., and Farooqi M.2008. Reduction in germination and seedling growth of Thespesia populnea L. caused by lead and cadmium treatments. Pakistan Journal of Botany. 40(6): 2419-2426.

29-Kasim W.A. 2005. The Correlation between Physiological and Structural Alterations Induced by Copper and Cadmium Stress in Broad Beans (Vicia faba L.) Egyptian Journal .of Biology.7: 20-32.

30-Liu W-X., Li H-H., Li S-R., and Wang Y-W.2006. Heavy metal accumulation of edible vegetables cultivated in agricultural soil in the suburb of Zhengzhou city, People’s Republic of China. Bulltain of Environmantal Contamination and Toxicology. 76:163–170.

31-Lindsay W.L., and Norvell W.A.1978. Development of a DTPA soil test for zinc, iron, manganez and copper. Soil Science Society of American Journal. 42: 421-428.

32-Małkowski E., Kurtykal R., Kita A.,and Karcz W. 2005. Accumulation of Pb and Cd and its effect on Ca distribution in maize seedlings (Zea Mays L.). Polish journal of Environmental Studies. 14(2): 203-207.

33-Meharg A.A.1993. Integrated tolerance mechanisms constitutive and adaptive plant response to elevated metal concentrations in the environment. Plant, Cell and Environment.17: 989-993.

34-Mitchell P. 1979. Keilin`s respiratory chain concept and its chemiosmotic consequences. Science. 206: 11-48.

35-Mihalescu LA., Mare-Rosca O.E., Marian M., and Bildar C.F. 2010.Research on the growth intensity of the zea mays L. plantlets aerial parts under cadmium treatment. Analele Universitatii din Oradea, Fascicula Biologie. 147-151.

36-Muchuweti M., Birkett J. W., Chinyanga E., Zvauya R., Scrimshaw M.D., and Lester J.N. 2006. Heavy metal content of vegetables irrigated with mixture of wastewater and sewage sludge in Zimbabwe: implications for human health. Agriculture, Ecology and Environment. 112: 41–48.

37-Nowak J. 2007. Effect of cadmium and lead concentrations and arbascular mycorrhiza on growth, flowering and heavy metal accumulation in Scarlet Sage(Salvia splendens Sello Torrreador). Acta Agrobotanica. 60(1):79-83.

38-Obata H., Inone N., and Umebayshi M. 1996. Effect of cadmium on plasma membrane ATPase from plant root differing in tolerance to cadmium. Soil Science. Plant. Nutrition. 42, 361-366.

39-Padmaja K., Parsad D.K., and Parsad A.R.1990. Inhibition of chlorophyll synthesis in Phaseolus vulgaris L. seedlings by cadmium acetate. Photosynthetica. 24: 399–404.

40-Pandey S., Gupta K., and Mukharjee A.K. 2007. Impact of cadmium and lead on Catharanthus roseus- A phytoremediation study. Jornal of Environmental Biology. 28:655-662.

41-Patra M., Bhowmik N., Bandopadhyay B., and Sharma A. 2004. Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance. Environmental and Experimental Botany. 52: 199-223.

42-Punz W. F., Sieghardt H.1993. The response of roots of herbaceous plant species to heavy metals. Environtal and Expermintal Botany. 33: 85-98.

43-Rayan J.R., Estefan G., and Rashid A. 2001. Soil and Plant Analysis Laboratory Manual. (2nd edition). ICARDA. Syria. P.172.

44-Rotkittikhun P., Kruatrachue M., Pokethitiyook P., and Baker A.J.M.2010.Tolerance and accumulation of lead in Vetiveria zizanioides and its effect on oil production. Journal of Environmental Biology. 31: 329-334.

45-Sanita di Toppi L. and Gabbrielli R. 1999. Response to cadmium in higher plants. Environmental and Experimental Botany. 41:105-130.

46-Scora R W., Chang A C (1997). Essential oil quality and heavy metal concentrations of peppermint grown on a municipal sludge-amended soil. Journal of Environmental Quality. 26(4): 975-979.

47-Shah, F. R., Ahmad N., Masood K.R., and Zahid D.M. 2008. The influence of cadmium and chromium on the biomass production of shisham (Dalbergia sissoo ROXB.) seedlings. Pakistan. Journal of Botany. 40(4): 1341-1348.

48-Shanker A. K., Cervantes C., Loza-Tavera H., and Avudainayagam S. 2005. Chromium toxicity in plants. Environment International. 31: 63-68.

49-Singh S., Singh A., and Bahadur R. 2011. Effect of cadmium on germination and seedling growth of tomato (Lycopersicum esculentum Mill). Plant Arch. 11(2): 859-862.

50-Soghoian S, R. Sinert. 2009. Toxicity, heavy metals.http://emedicine.medscape.com/article/814960-overview.

51-Srivastava N. K. and Luthra R.1994. Relationship between photosynthetic carbon metabolism and essential oil biogenesis in peppermint under Mn stress. Journal of Experimental. Botany.45: 1127-1132.

52-Stancheva M.G., Hristozkova M., Markovska Y., and Salamon I. 2010.Antioxidant Capacity of sage grown on heavy metal polluted Soil. Russian Journal of Plant Physiology.57(6):799–805.

53-Stancheva M., Geneva M., Hristozkov M., Boychinova M., and Markovska Y. 2009. Essential oil varioations of Salvia officinalis L. grown on heavy metals polluted soil. Biotech. Biotech.EQ. XI Anniversary Science Conference. Special Edit. Online. 373-376.

54-Sun Y.,. Zhou Q, and Diao Ch.2008. Effects of cadmium and arsenic on growth and metal accumulation of Cd-hyperaccumulator Solanum nigrum L. Bioresearch and Tech.99: 1103–1110.

55-Tirillini B., Ricci A., Pintore G., Chessa M., and Sighinolfi S. 2006. Induction of Hypericins in Hypericum perforatum in Response to Chromium. Fitoterapia.77: 164-70.

56-Tiryakioglu M., Eker S., Ozkutlu F. 2006. Antioxidant defense system and cadmium uptake in barley genotypes differing in cadmium tolerance. Journal of Trace Element. Medicine and Biology. 20: 181-189.

57-Topalov V. and Zhelyazkov V. 1991. Effect of harvesting on the yield of fresh material, essential oil, and planting material from Mentha piperita L. and Mentha arvensis L. Herba. Hung 50: 60-67.

58-Van Assche F., Clijsters H.1990. Effects of heavy metals on enzyme activity in plants. Plant and Cell Environmen. 13: 195-206.

59-Vassilev A. and Yordanov I.1997. Reductive analysis of factors limiting growth of cadmium –treated plants: A Review:Bulgarian Journal Plant Physiology. 23(3-4): 114-133.

60-Vassilev A., Schwitzguebel J.P., Thewys T., Van der Lelie D., and Vangronsveld J. 2004. The Use of plants for remediation of metal-contaminated soils. Rev. The Science World Journal. 4:9–34.

61-Wei S.H., Zhou Q.X. 2004. Discussion on basic principles and strengthening measures for phytoremediation of soils contaminated by heavy metals. Chin. J. Ecol. 23: 65–72 (in Chinese).

62-Wierzbicka M. 1995. How lead loses its toxicity to plant. Acta Socienty Botany. 64: 81-90.

63-Wierzbicka1 M. 1998. Lead in the apoplast of Allium cepa L. root tips.ultrastructural studies. Plant Science.133:105.

64-Yang X.E., Long X.X., Ye H.B., He Z.L., Calvert D.V., Stoffella P.J. 2004. Cadmium tolerance and hyperaccumulation in a new Znhyperaccumulating plant species (Sedum alfrdii Hance.). Plant and Soil. 259: 181–189.

65-Zheljazkov V.D., and Nielson N.E. 1996. Effect of heavy metals on peppermint and cornmint. Plant and Soil. 178: 59–66.

66-Zheljazkov V.D. Jeliazkova E.A., Kovacheva N., and Dzhurmanski A. 2008. Metal uptake by medicinal plant species grown in soils contaminated by a smelter, Environmental Expermental Botany. 64: 207–216.

67-Zheljazkov V.D., Craker L.E., and Xing B. 2006. Effects of Cd, Pb, and Cu on growth and essential oil contents in dill, peppermint, and basil Environmental and Experiment Botany. 58: 9–16.

68-Zheljazkov V.D., and Nielsen N.E. 1993. Studies on the Effect of Heavy Metals (Cd, Pb, Cu, Mn, Zn and Fe) Upon The Growth, Productivity and Quality of Lavander (Lavandula vera D. C.) Production. A Paper Presented at the 24th International Symposium on Essential Oils. Berlin. Journal of Essential Oil Research. (In press).

69-Zheljazkov V D, and Warman P.R.2003. Source-Separated Municipal Solid Waste Compost 70-Application to Swiss Chard and Basil. Heavy Metals in the Environment. Technical Report.

70-Zheljazkov V.D., Warman P.R.2003. Application of High Cu Compost to Swiss Chard and Basil. Science Total Environment. 302:13–26.