Distribution of Phosphate Solubilizing Bacteria and Soil Phosphatase Activity in Different Land Uses

Document Type : Research Article

Authors

• Mohammad Reza Sarikhani ¹
• N. Chalabianlu ²
• S. S. Alavikia ²

¹ Tabriz

² University of Tabriz

https://doi.org/10.22067/jsw.v29i6.39566

Abstract

Introduction: Phosphorous is one of the essential macronutrients for plant growth and development but its mobility in soil is very low. The utilization of the soil biological potential, in particular phosphate solubilizing bacteria, is an efficient way which can be used for exploiting available sources of phosphorous in the soil. The principal mechanism for mineral phosphate solubilization is the production of organic acid, and acid and alkaline phosphatases play a major role in the mineralization of organic phosphorous in the soil. Presence and distribution of phosphate solubilizing bacteria in the soil and soil phosphatase activities is influenced by soil conditions such as climate, soil type, vegetation and land uses. In order to understand the relationships and considering the importance of the subject, the soil samples were chosen from two different climates; semi-moist (Fandoghlou-Ardabil) and semi-arid (Namin- Ardabil) under culture of legumes, cereals and uncultivated areas, in this experiment.
Materials and Methods: In order to study the effects of different land uses, climate conditions and soil physicochemical properties on phosphate solubilizing microorganism (PSM) distribiution and soil acid and alkaline phosphatase activity, a factorial experiment based on completely randomized design was performed with considering three different land uses (including legumes, cereals and wasteland) and two climate conditions (semi-moist: Fandoghlu- Ardabil and semi-arid: Namin-Ardabil). Four composite soil samples (0-25 cm) were taken from each land uses. Finally, a total number of 24 soil samples were used to enumerate phosphate solubilizng bacteria and evaluate soil phosphatase activities. The enumeration and selection of bacteria in the mineral Sperber medium was done by attention to the clear zone production in the presence of tri-calcium phosphate and in organic sperber (IHP+BCIP) due to blue phenotype of grown colonies. Also, phosphatase activity of soil samples was assessed based on the usual methods for phosphatase assessment. Moreover, after the evaluation of the physicochemical properties of the soil samples and soil enzyme activities and PSB distribution, all data were analyzed by SPSS and MSTAT-C softwares.
Results and Discussion: The Sperber mediums containing mineral and organic phosphates were used in counting the number of PSB. According to the results, the highest total number of bacteria (>6 log cfu/g) was gained in legume land uses in both climate conditions. Furthermore, the highest numbers of organic and mineral phosphate solubilizing bacteria (5.3 log cfu/g) were counted in samples taken from pastures, in other word in soil samples which were collected from uncultured land in semi-moist climate conditions. Enzyme assay showed that acid phosphatase activity (430 µg pNP/g.h) in semi-moist climate conditions were increased three times in comparison to semi-arid climate conditions. Perhaps, this increase can be explained by parameters such as high moisture content and organic matter which can cause an increase in the number of bacteria and soil enzyme content. Our results showed that, alkaline phosphatase activities (APAs) were affectd by interaction effects of land uses and climate, wherein the highest APA (810 µg pNP/g.h) was measured in legume samples in semi-arid climate conditions. The pH of these soil samples and supporting legume rhizospheres from AP producing microorganisms may be the reasons of this increment.
Conclusion: The highest activity of the soil acid phosphatase was observed in soil samples which were taken from uncultivated area under semi-moist climate conditions (namely pastures) (866.59 µgPNP/g.h) while the soil alkaline phosphatase activity had high mean in legume land use soil samples under semi-arid climate conditions (795.15 µPNP/g.h). The total number of bacteria was the highest in semi-arid leguminous land use (14.13×106 cfu/g) but the total numbers of solubilizing bacteria in both mineral and organic media were the highest in semi-moist uncultivated area (respectively 1.9 ×106 and 1.48 × 106cfu/g).

Keywords

• Acid phosphatase
• Alkaline phosphatase
• phosphate
• Phosphate Solubilizing Bacteria

20.1001.1.20084757.1394.29.6.23.6

References

1- Chavoshi A., Khademi H., and Norbakhsh F. 2004. Land usage type and Land Scape position effects on alkaline phosphatase activity. Agriculture Science, 35 (3): 741-751. (in Persian with English abstract)

2- Sarikhani M.R., and Malboobi M.A. 2011. Phytases: enzymology, molecular and biochemical characteristic and applications. Journal of Agricultural Biotechnology. 2 (2): 13-39. (in Persian with English abstract)

3- Sarikhani M.R., Malboobi. M.A., and Ebrahimi M. 2014. Phosphate solubilizing bacteria: Isolation of Bacteria and Phosphate Solubilizing Genes, Mechanism and Genetics of Phosphate Solubilization. Journal of Agricultural Biotechnology. 6(1): 76-110. (in Persian with English abstract)

4- Fallah Nosrat Abad A. 2012. Evaluation of relationships between soil properties and total bacteria and fungi in soils of Guilan. Journal of Soil Management and Sustainable Production. 2 (2): 49-68. (in Persian with English abstract)

5- Fallah Nosrat Abad A., Rahimian H., and Saleh Rastin N. 2001. Study of phosphate solubilizing microorganism’s distribution in some soils of Gilan province, Water and Soil Science, 17 (2): 345-383. (in Persian with English abstract)

6- Gollar Ata M., Raiese F., and Nadian H. 2008. Salinity and phosphorus interactions on growth, yield and nutrient uptake by berseem clover (Trifolium alexandrinum L.). Journal of Agronomic Researchs of Iran, 22 (2): 217-223. (in Persian with English abstract)

7- Amador J.A., Glucksman A.M., Lyons J.B. and Gorres J.H. 1997. Spatial distribution of soil phosphatase activity within a riparian forest. Soil Science, 162 (11): 808-823.

8- Baby U.I., Tensing Baliah N., Ponmurugan P. and Premkumar R. 2001. Population dynamics of nitrogen fixing and phosphate solubilizing bacteria in tea Soil. UpASI Tea Res. Found. Newsletter, 10:4.

9- Bandick A.K., and Dick R.P. 1999. Field management effects on soil en. zyme activities. Soil Biology Biochemistry 31: 1471-1479.

10- Cui J., and Holden N.M. 2015. The relationship between soil microbial activity and microbial biomass, soil structure and grassland management. Soil and Tillage Research, 146A: 32–38.

11- Dick R.P., Doran J.W., Coleman D.C., Bezdicek D.F., and Stewart B.A. 1994. Soil enzyme activities as indicators of soil quality. American Journal of Soil Science Society. Special Publication, 35: 107-124.

12- Dick R.P., Myrold D.D., and Kerle E.A. 1988. Microbial biomass and soil enzyme activities in compacted and rehabilitated Skil trail soil. American Journal of Soil Science Society, 52: 512-516.

13- Fageria N.K. 2009. The use of nutrients in crop plants. Taylor and Francis Group, LLC. New York.

14- Gee G.W., and Bauder J.W. 1986. Particle size analysis. Methods of Soil Analysis. Part1, Physical and mineralogical methods. American Society Agronomy Publication. 383-411.

15- Gupta V.V.S.R., and Germida J.J. 1988. Distribution of microbial biomass and its activity in different soil aggregate size classes as affected by cultivation. Soil Biol. Biochem. 20: 777-786.

16- Han X.M., Wang R.Q., Liu J., Wang M.C., Zhou J., and Guo W.H. 2007. Effects of vegetation type on soil microbial community structure and catabolic diversity assessed by polyphasic methods in North China. Journal of Environmental Sciences, 19(10): 1228–1234.

17- Hedlund K. 2002. Soil microbial community structure in relation to vegetation management on former agricultural land. Soil Biology and Biochemistry, 34: 1299–1307.

18- Johnson N.C., Copeland P.J., Crookston R.K., and Pfleger F.L. 1992. Mycorrhizae: Possible explanation for yield decline with continuous corn and soybean. Agron. J., 84: 387-390.

19- Khan A., Jilani G., Akhtar M.S., Saqlan Naqvi S.M., and Rasheed M. 2009. Phosphorus solubilizing bacteria: Occurrence, Mechanisms and their role in crop production. Agricultural biology Science, 1(11): 48-58.

20- Liang Q., Chen H., Gong Y., Yang H., Fan M., and Kuzyakov Y. 2014. Effects of 15 years of manure and mineral fertilizers on enzyme activities in particle-size fractions in North China Plain soil. European Journal of Soil Biology, 60: 112-119.

21- Malboobi M.A., Owlia P., Behbahani M., Sarokhani E., Moradi S., Yakhchali B., Deljou A., and Morabbi Heravi K. 2009. Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates. World Journal of Microbiology and Biotechnology, 25: 1471–1477.

22- Monreal C.M., and Bergstrom D.W. 2000. Soil enzymatic factors expressing the influence of land use, tillage system and texture on soil biochemical quality. Canadian Journal of Soil Science 80: 419-428.

23- Omidi H., Tahmasebi Z., Torabi H., and Miransari M. 2008. Soil enzyme activities and available P and Zn as affected by tillage practices, Canola (Brassica napus L.) cultivars and planting dates. European Journal of Soil Bioloy, 44: 443-450.

24- Pandey D., Agrawal M., and Bohra J.S. 2014. Effects of conventional tillage and no tillage permutations on extracellular soil enzyme activities and microbial biomass under rice cultivation. Soil and Tillage Research, 136: 51-60.

25- Ponmurgan P., and Gopi. C. 2006. Distributation pattern and screening of phosphate solubilizing bacteria selected form different food and forage crops. Journal of Agronomy, 5(4): 600-604.

26- Rodrıguez H., and Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances, 17: 319 –339.

27- Sarikhani M.R., Malboobi M.A., Aliasgharzad N., Greiner R., and Yakhchali. 2010. Functional screening of phosphatase-encoding gene from bacterial sources. Iranian Journal of Biotechnology 8(4): 275-279.

28- Shahbazi F., Aliasgharzad N., Ebrahimzad S.A., and Najafi N. 2013. Geostatistical analysis for predicting soil biological maps under different scenarios of land use. European Journal of Soil Biology, Volume 55. 20-27.

29- Sperber J.I. 1985. The incidence of apatite solubilizing organisms in the rhizosphere. Australian Journal of Agricultural research, 9: 778-781.

30- Tabatabai M.A., Weave R.W., Angle S., Bottomley P., Bezdicek D., Smith S., Tabatabai A., and Wollum A. 1994. Soil enzyme, Methods in soil Analysis, part 2: Microbiological and Biochemical properties.

31- Wei K., Chen Z.H., Zhang X.P., Liang W.J. and Chen L.J. 2014. Tillage effects on phosphorus composition and phosphatase activities in soil aggregates. Geoderma, 217-218: 37-44.

32- Xue-mei H., Ren-qing W., Jian L., Meng-cheng W., Juan Z., and Wei-hua1 G. 2007. Effects of vegetation type on soil microbial community structure and diversity assessed by polyphasic methods in North China. J. Environ. Sci., 19: 228–1234.