Evaluation of Freezing Tolerance of English Daisy (Bellis perennis) under Controlled Conditions

Document Type : Research Article

Authors

1 Department of Horticulture Sciences, Faculty of Agricultural, Ferdowsi University of Mashhad

2 Khosheparvaran Zistfanavar

3 Department of Agronomy and Plant Breeding, Faculty of Agricultural, Ferdowsi University of Mashhad

4 Faculty of Agriculture, Ferdowsi University of Mashhad

5 , Faculty of Agricultural, Ferdowsi University of Mashhad

Abstract

Abstract
The aim of this experiment was the study of freezing tolerance of Bellis perennis under controlled conditions and were arranged in a completely randomized design with three replications. Plants after sowing and grow in the bed, at the middle of autumn (after hardening in natural conditions), in the 7-8 leaf stage put on the thermogradiant freezer with the 12 freezing temperatures (0,-2,-4,-6,-8,-10,-12,-14,-16,-18,-20,-22 oC). Cell membrane stability was measured through electrolyte leakage (EL) and survival percentage and regrowth of the plants after 3 weeks in cold frame were measured, by counting the number of plants and determining their proportional with the number of plants before freezing and measuring the dry matter, number of flowers and fully developed leaves. With decreasing the temperature EL increased significantly (P

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