A. Golestani Fard; H. Mirseyed Hosseini; Gholam Reza Savaghebi; Gh.R. Savaghebi
Abstract
In this research chemical forms of lead and zinc in bulk and rhizosphere soil of different cultivars of maize and canola were determined by sequential and single step extraction methods. Some factors affecting chemical transformation of the two elements such as pH, dissolved organic carbon (DOC), their ...
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In this research chemical forms of lead and zinc in bulk and rhizosphere soil of different cultivars of maize and canola were determined by sequential and single step extraction methods. Some factors affecting chemical transformation of the two elements such as pH, dissolved organic carbon (DOC), their uptake by plant and also cation exchange capacity in the rhizosphere and bulk soil were also assessed. Rhizosphere was obtained with gentle shaking and separating the soil around plant roots. Results showed that zinc uptake was more than lead in both plant cultivars. Zinc and lead accumulation in all cultivars roots were also more than shoots. Translocation index (The ratio of element concentration in shoot to root) in the corn cultivars was higher than canola cultivars (significant correlation= 1%) while the ratio was higher for lead in canola cultivars. Metal concentration in shoots to total metal concentrations in soil (Accumulation factor) showed a similar trend like the translocation index. In total, the corn ability in zinc and lead absorption and extraction in soil was higher compared to canola. There weren’t any significant changes in rhizosphere pH compared to bulk soil. Dissolved organic carbon in the rhizosphere of different cultivars was more than bulk soil and the amount of it in canola was significantly more than corn cultivars. Among each plant cultivars, the absorption rate increased with increasing root exudates. The results of sequential and single step extraction methods showed that the general trend and the soil system tendency (Root-Soil interface) is toward zinc availability in the rhizosphere. The amount of lead in rhizosphere showed no significant changes to bulk soil with none of the extractants in single step extraction method but with regards to the results of sequential extraction method, rhizosphere processes tend to reduce the availability of this element in both plant cultivars.
A. Bostani; Gh.R. Savaghebi
Abstract
Abstract
This study was conducted to investigate, under incubation conditions (FC moisture and temperature of 30o c), the effect of the potassium concentration and the cultivation background on the K- fixation in Khuzestan’s sugarcane under-cultivation soils. For this reason, 70 complex soil samples ...
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Abstract
This study was conducted to investigate, under incubation conditions (FC moisture and temperature of 30o c), the effect of the potassium concentration and the cultivation background on the K- fixation in Khuzestan’s sugarcane under-cultivation soils. For this reason, 70 complex soil samples in 35 pairs each coupled out of two different depths (0-30 and 30-60 cm), chosen from the under-cultivation soils of 3 different farmland: Haft Tappeh Agro- industry (with a maximum cultivation background of 41 years), Karun (with a maximum cultivation background of 30 years), and sugarcane Development Plan (with a maximum cultivation background of 8 years) were supplied. In order to study of the effect of the Potassium concentration and the sugarcane cultivation background on the K- fixation, a factorial experiment with 4 levels of the K concentration (0, 150, 300, 600, mg kg -1), and 4 levels of the cultivation background (less than 10 years, 10-20, 20-30, and more than 30) in based on completely randomized design was conducted. The results showed that the amount of Potassium fixation ranged from 17.74 mg kg -1 to 129.15 mg kg-1. With an increase in the potassium concentration, the amount and percentage of the K- fixation were both increased (p
B. Motesharezadeh; Gh.R. Savaghebi
Abstract
Abstract
Nowadays, soil pollution with heavy metals has become a major problem which their accumulation in plants can directly or indirectly influence on animals and humans life. Lead and cadmium are poisonous metals, with increasing the amount of these metals in environmental pollution; they will be ...
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Abstract
Nowadays, soil pollution with heavy metals has become a major problem which their accumulation in plants can directly or indirectly influence on animals and humans life. Lead and cadmium are poisonous metals, with increasing the amount of these metals in environmental pollution; they will be increased in food chain significantly. In this research, three levels of cadmium (0, 100 and 200 mg/kg), three levels of lead (0, 200 and 400 mg/kg) and three levels of native resistance bacteria inoculants (B0: Control, B1: Bacillus mycoides and B2: Bacillus circulans ) were examined as a factorial experiment with randomized complete block design (RCBD) in three replications. Then sunflower responses to cadmium and lead toxicity including: heavy metal and micronutrients concentration and their uptakes and finally dry matter weight of sunflower were evaluated in greenhouse condition. Results showed that with increasing cadmium and lead concentration in soil, concentration of these elements in plant root and shoot increased significantly (P
E. Entezami; M. Shorafa; Gh.R. Savaghebi
Abstract
Abstract
Soil pollution with heavy metals like zinc causes increasing concerns about environment. According to distribution in soil, this study was conducted to compare zinc concentration between aggregate exteriors and interiors of some contaminated soils. Aggregates were collected from 5 depths of ...
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Abstract
Soil pollution with heavy metals like zinc causes increasing concerns about environment. According to distribution in soil, this study was conducted to compare zinc concentration between aggregate exteriors and interiors of some contaminated soils. Aggregates were collected from 5 depths of 4 soil profiles with different textures and were separated mechanically into exterior and interior fractions. Soluble and exchangeable and also bonded to carbonate fractions of zinc from both aggregate interiors and exteriors, were extracted and measured. The concentrations of zinc in both above fractions were high in the exterior part. Concentration of soluble and exchangeable zinc showed high correlation coefficient with soil pH and total concentration of soil zinc, also bonded to carbonate fraction of zinc showed high correlation coefficient with total concentration of zinc and low correlation coefficient with percentage of soil CaCO3.
Keywords: Aggregate, Contaminated soil, Forms of zinc
Z. Farzanegan; Gh. Savaghebi; H. Mirseyed Hosseini
Abstract
Abstract
The use of plants to remove contaminants from the environment and concentrate them in above-ground plant tissue is known as phytoextraction. This study was conducted to investigate the potential of phytoextraction of Pb and Cd metals canola (Brassica napus) and sorghum (Sorghum bicolor) which ...
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Abstract
The use of plants to remove contaminants from the environment and concentrate them in above-ground plant tissue is known as phytoextraction. This study was conducted to investigate the potential of phytoextraction of Pb and Cd metals canola (Brassica napus) and sorghum (Sorghum bicolor) which are high Biomass Crop and the effect of amendments on the efficiency of phytoextraction. The soil sample for the experiment was collected from an area adjacent to a Zinc Concentrate factory in west of Zanjan, Iran. Five treatments compared included powdered sulfur, sulfur plus thiobaccilus inucolum (S+I) at 8g kg -1 rate, citric acid at two levels of 1g kg-1 (CA1) and 3g kg-1 (CA2) and the control (C). The experiment was conducted in two stages. In the first stage of study, the treated soil with the above material were incubated for 8 weeks at field capacity and 25˚C temperature. In the incubation period the subsamples were collected at 0, 4 and 8 weeks to determine the amount of plant available Pb and Cd using DTPA extraction. In the second stage, a greenhouse experiment was conducted using the same soil and treatments. Sorghum and canola were planted and grown for 60 days and then the above ground parts and roots were harvested. Plant samples were analyzed for Cd and Pb content and the amount of DTPA exractable Pb and Cd were measured in soils before and after planting. Both experiments were conducted in the factorial experiment with randomized complete design with 5 treatments (including control) and 4 replications. The statistical Analysis was done using SAS and MSTATC Softwares. The resulst indicated that effect of sulfur plus thiobaccilus and citric acid at 1g.kg-1 treatments on Cd and Pb availability were highest and lowest, respectively. The pot experiment showed that the best of amendment in transfer of metal from root to shoot for two plant species was powdered sulfur plus thiobaccilus inucolum. The results of greenhouse experiment indicated that canola has higher potential for remediation of Cd and Pb from contaminated soil in comparison with sorghum.
Keywords: Lead, Cadmium, Sorghum, Canola, Phytoextraction, Amendment
E. Malekzadeh; H.A. Alikhani; Gh.R. Savaghebi; M. Zarei
Abstract
Abstract
In this study, interaction between AMF (G. mosseae and Glomus spp., respectively indigenous and non-indigenous of HM-contaminated areas) with Cd-resistant PGPRs (Bacillus mycoides and Micrococcus roseus, indigenous of contaminated areas) on the growth, Cd and nutrient uptake of maize plant ...
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Abstract
In this study, interaction between AMF (G. mosseae and Glomus spp., respectively indigenous and non-indigenous of HM-contaminated areas) with Cd-resistant PGPRs (Bacillus mycoides and Micrococcus roseus, indigenous of contaminated areas) on the growth, Cd and nutrient uptake of maize plant (Zea mays L.) in Cd polluted soil were investigated. With increasing levels of Cd, shoot and root dry weights, shoot Fe and P contents decreased but root and shoot Cd content increased. Root colonization was varied at different levels of Cd and co-inoculation with PGPRs. G. mosseae treatment had greatest amount of shoot and root dry weight, Fe and P of shoot at high concentration of Cd. At the levels of 100 and 200 Cd, in only mycorrhizal treatments, plants colonized by Glomus spp. and G. mosseae had respectively high content of Cd roots. At both levels of Cd, shoot Cd content in co-inoculation of M. roseus and B. mycoides with G. mosseae increased and decreased respectively in comparison with single inoculation of G. mosseae. While, at the levels of 100 and 200 Cd, shoot Cd content in co-inoculation of PGPRs with Glomus spp. respectively increased and decreased/did not significant different compared to single inoculation of Glomus spp. Co-inoculation of G. mosseae and M. roseus, with maximum Cd-accumulated in plant, was the most effective treatment in Cd phytoremediation and stabilization.
Keywords: AM fungi, Plant growth promoting rhizobacteria, Phytoremediation, Cd and maize
A.H. Khoshgoftar Manesh; E.S. Razizadeh; H.R. Eshghizadeh; Gh.R. Savaghebi; A. Sadrearhami; D. Afuni
Abstract
Abstract
This study was conducted at Rudasht Research Farm in Isfahan to evaluate tolerance to zinc (Zn) deficiency of 30 spring wheat genotypes, using split plots in a randomized complete blocks design with triplications. Main plot consisted of two Zn levels (Zero and 80 kg Zn ha-1 applied as zinc ...
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Abstract
This study was conducted at Rudasht Research Farm in Isfahan to evaluate tolerance to zinc (Zn) deficiency of 30 spring wheat genotypes, using split plots in a randomized complete blocks design with triplications. Main plot consisted of two Zn levels (Zero and 80 kg Zn ha-1 applied as zinc sulfate at planting). The results of ANOVA as well as correlations between tolerance and susceptibility indices to Zn deficiency stress and grain yield indicated that MP, GMP and STI were the most suitable indices for identifying and selecting Zn-deficiency tolerant wheat genotypes. Both at with and without added Zn treatments, grain yield had significant positive correlation with MP, GMP and STI. The STI could separate the studied wheat genotypes in different groups based on both their response to fertilization and grain yield potential. Therefore, the STI was the best index to identify Zn deficiency tolerant genotypes. Based on three indices, Ghods and Falat genotypes were the most tolerant and sensitive genotypes to Zn deficiency, respectively.
Keywords: Stress indicator, Wheat, Zinc efficiency, Tolerance
.A Sadat; Gh. Savaghebi; F. Rejali; M. Farahbakhsh; K. Khavazi; M. Shirmardi
Abstract
Abstract
The objective of this study was to assess the effects of few arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria on the growth and yield indices of two wheat varieties in a saline soil (EC=10/1 dSm-1). A factorial experiment with completely randomized design with four replications ...
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Abstract
The objective of this study was to assess the effects of few arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria on the growth and yield indices of two wheat varieties in a saline soil (EC=10/1 dSm-1). A factorial experiment with completely randomized design with four replications was conducted to investigate the effects of three levels of fungal inoculation (non inoculation , inoculation with Glomus etunicatum and with Glomus intradices) and four levels of bacterial inoculation (non inoculation, inoculation with P. fluorescens strains 4 , 9, 12) on two wheat varieties (Sistan and Chamran) as tolerant and semi-tolerant to salinity, respectively. Our results showed that the growth and yield indices of two varieties were significantly (P
F. Moshiri; M. Ardalan; M. Tehrani; Gh. Savaghebi
Abstract
Abstract
Due to calcareous soil conditions, zinc deficiency is a yield limiting factor for wheat production in Iran. One solution for this problem is the cultivation of Zn-efficient wheat varieties. Ten wheat cultivars (nine bread wheat and one durum wheat) with different tolerance to Zn deficiency ...
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Abstract
Due to calcareous soil conditions, zinc deficiency is a yield limiting factor for wheat production in Iran. One solution for this problem is the cultivation of Zn-efficient wheat varieties. Ten wheat cultivars (nine bread wheat and one durum wheat) with different tolerance to Zn deficiency were studied in a greenhouse experiment. Plants were treated with Zn (10 mg Zn Kg-1 soil as ZnSO4.7H2O) and without it. Based on the results of pot experiment, two wheat cultivars with different Zn efficiency were selected to assess the ability of phytosiderophore release from their roots. Visual Zn deficiency symptoms, such as light colored necrotic patches and reduction in plant height appeared more severe in Alvand, Mahdavi (bread wheat) and Yavares (durum wheat) cultivars. Under Zn deficiency, shoot dry matter decreased in most cultivars. Zn efficiency of wheat cultivars ranged from 80.4% to 106.2%. Based on shoot dry weight, Pishtaz and Darab were the most Zn-efficient and Alvand and Mahdavi were the most Zn-inefficient wheat cultivars. Zn supply markedly increased the Zn concentration and content of shoots of all cultivars. Cultivars differed in Zn uptake under Zn-deficient and Zn-sufficient conditions. On average, Zn-efficient cultivars had more Zn uptake efficiency compared to Zn-inefficient cultivars. Our results strongly suggested that sensitivity to Zn deficiency varied widely among wheat cultivars. Different susceptibility of cultivars did not correspond well with the Zn concentration of the shoot. In contrast, the total amount of Zn of shoot was better related to the sensitivity of wheat cultivars to Zn deficiency. Under Zn deficiency, different ability of genotypes to release phytosiderophore from roots was observed. Pishtaz exuded more phytosiderophore than Alvand. Higher Zn uptake in Zn-efficient wheat genotypes may be attributed to higher release rates of Zn-mobilizing phytosiderophores from roots.
Keywords: Wheat cultivars, Zinc efficiency, Zinc concentration, Zinc uptake
F. Khosravui; Gh.R. Savaghebi; H. Farahbakhsh
Abstract
Abstract
The use of plants for remediation of heavy metals contaminated soils is an economical, cheap and effective strategy. This research was conducted in order to study the effect of potassium chloride on cadmium uptake and enhancement of it's phytoremediation by canola (Brassica napus L.) and sunflower ...
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Abstract
The use of plants for remediation of heavy metals contaminated soils is an economical, cheap and effective strategy. This research was conducted in order to study the effect of potassium chloride on cadmium uptake and enhancement of it's phytoremediation by canola (Brassica napus L.) and sunflower (Helianthus annuus L.) in a cadmium polluted soil from Angoran in Zanjan province. In a randomized complete block design (RCBD), with three replications, four levels of potassium (0(control), 50,100 and 200 mg K2O per kilogram of soil as KCl) was performed and Azargoal hybrid variety of sunflower and Double Zero variety of spring canola separately were planted in pot. The ANOVA results showed that application of KCl treatments have significantly effects (P
