vahid alah jahandideh mahjan abadi; alidad karami; sayed roholla mousavi; H. Asadi Rahmani
Abstract
Introduction:Soil quality as an important part from soil resource sustainability, consistently isinfluenced by human activities.Today, the presence of accurate information about variability of soil quality properties is considered more than ever to apply this information in economic modeling, environmental ...
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Introduction:Soil quality as an important part from soil resource sustainability, consistently isinfluenced by human activities.Today, the presence of accurate information about variability of soil quality properties is considered more than ever to apply this information in economic modeling, environmental predictions, accurate farming and natural resources management. Soil quality is defined as: “capacity of the soil to function, within the ecosystem and land-use boundaries, to sustain biological productivity, maintain environmental quality, and promote plant and animal health”; therefore, it is one of the most important factors in developing sustainable land management and sustaining the global biosphere. The definition of soil quality encompasses physical, chemical and biological characteristics, and it is related to fertility and soil health. Many indicators can be used to describe soil quality, but it is important to take into account sensitivity, required time, and related properties, than can be explained. Properties related to organic matter content, such as microbial respiration, microbial biomass carbon (MBC) and enzymatic activity (urease and phosphatases) can be used as soil quality indicators. They provide early information about mineralization processes, nutrient availability and fertility, as well as effects resulting from changes in land use or agricultural practices (e.g. tillage or application of different types of organic matter). In this context, biological properties have been used as soil quality indicators, because of their relationship with organic matter content, terrestrial arthropofauna, lichen, microbial community (biomass or functional groups), metabolic products as ergosterol or glomalin and soil activities as microbial respiration and enzyme production. This study was carried out for evaluation the spatial variability of biological soil quality indicators in wheat farms of Pasargad plain.
Materials and Methods: After reviewing the initial map of Pasargad, a total of 60 samples were provided using a systematic grid square sampling pattern with 500×500 m over the 1200 ha area of Pasargad at surface soil depth (0-30 cm). The characteristics of soil including organic carbon, pH, EC, microbial respiration, microbial biomass carbon , soil alkaline phosphatase and urease enzymes activity, ratio of microbial biomass carbon to organic carbon (MBC/OC) andmicrobial metabolic quotient(qCO2) were measured and calculated. Results were analysed with SPSS, Excel, GS+, and ArcGIS sotwares. Summary statistics were calculated for the 60 samples including mean, maximum and minimum, coefficient of variation (CV), kurtosis and skewness. In addition, Pearson correlation coefficients were calculated for untransformed data. For evaluation of different interpolation methods of soil characteristics in Pasargad plain root mean square error (RMSE), mean bias error (MBE) and mean absolute error (MAE) were used. We also constructed maps of the spatial distributions for each individual variable using best interpolators including kriging, inverse distance weighting (IDW) and cokriging methods.
Results and Discussion; The results showed that in the most cases the studied properties had too much variation. Based on the coefficient of variation, pHand qCO2had the lowest and highest variations, respectively. There was significant linear correlation between most of soil properties. From lognormal transformation was used for normalization of EC and qCO2. Best model for single semivariogram of organic carbon, microbial respiration, urease enzyme activity, microbial biomass carbon, qCO2 and MBC/OC in the soil was spherical model, for pH in the soilwas exponential model and for EC and phosphatase enzyme activity was gaussian model. Also, the best interpolator for pH, EC, organic carbon, microbial biomass carbon, urease activity, qCO2and MBC/OC was kriging, for alkaline phosphatase activity was inverse distance weight, and for microbial respiration was cokriging method. Amount of pH increased from north to south of Pasargad plain, but amounts of EC and organic carbon were inverse of pH.The higher amounts of microbial respiration and urease activity were observed at the south and east, respectively. The amount of phosphatase activity in the soil of Pasargad plain was scattered, and wide area in the plain had the activity between 215-275 µg PNP/g.hr. The higher amount of MBC and MBC/OCand lower amount of qCO2were observed at the west.
Conclusions: The biological soil properties were sensitive and rapid indicators of effects of soil management. Generally, according to the spatial variabilitymap, the areas in the region are critical situations in terms of biological indicators of soil. So the management techniques that are applied by farmers in these areas have to be changed. The results of this study used in the improvement of regional planning for sustainable management of soil.
Vida Hemmati; Hadi Asadi Rahmani; Shokofeh Rezaee
Abstract
Introduction: Wheat is one of the most important food crops. In modern agriculture, due to the increase in human population and the detrimental effects of pesticides such as environmental pollution, concerns about human and animal health, adapting suitable alternatives which have none of these dangerous ...
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Introduction: Wheat is one of the most important food crops. In modern agriculture, due to the increase in human population and the detrimental effects of pesticides such as environmental pollution, concerns about human and animal health, adapting suitable alternatives which have none of these dangerous effects would be necessary. This is possible by increasing the production of bio-fertilizers. Plant growth-promoting rhizobacteria (PGPR) are the beneficial rhizosphere bacteria that can enhance plant growth directly or indirectly through a wide variety of mechanisms. PGPR can stimulate plant growth directly by supplying nutrients such as phosphorous and nitrogen or by the production of phytohormones such as auxins, cytokinins (CK), gibberellins (GAs) or ACC deaminase synthesis. They can also promote plant development indirectly by the suppression of pathogens by different mechanisms such as biosynthesis of antimicrobial molecules or antibiosis induced systemic resistance (ISR), rhizosphere competition, cell wall degrading enzymes like chitinase and HCN production. In this study, amplified ribosomal DNA restriction analysis was performed for screening the bacterial isolates. Then phosphate solubilization, siderophore and auxin release activities and effect of bacterial isolates on wheat seed germination traits were studied.
Materials and Methods: In order to isolate wheat rhizosphere bacteria, soil samples were taken from the wheat rhizosphere of Tehran, Qazvin, Zanjan, West and East Azerbaijan, Kurdistan and Hamadan provinces. Genomic DNA of each isolate was extracted by using a modified cetyl trimethylammonium bromide (CTAB) method. Amplified ribosomal DNA restriction analysis with HpaII and RsaI restriction enzymes was done for genetic screening. Growth stimulating factors were evaluated by auxin production, siderophore production, and inorganic phosphate solubilizing activity. Siderophore production was determined by measuring the diameters of the colony (mm) and of any orange halo (mm) formed from the blue medium surrounding bacterial growth on CAS Blue Agar medium. To examine Pi solubilization capability, 2µ bacteria suspension was placed on the plates containing Sperber’s medium. Cultures were incubated at 25 ± 2 °, when the diameters of the colony and of the halo zone surrounding it were measured and the mean ± SE of the ratios of halo (mm)/colony (mm) calculated. In order to evaluate the production of auxin, isolates were grown in 100ml flasks containing 25ml TSB medium for 48h on a rotary shaker. 1 ml supernatant was mixed with 2ml of Salkowsky reagent after centrifugation at 10000g for 15min. The absorbance of the complex was read at 535nm in a Spectrophotometer. To investigate the effect of bacterial isolates on germination traits, radicle and plumule fresh and dry weight, radicle and plumule length, germination percentage, germination rate, and germination average rate were measured. The data were analyzed with using SAS 9.1. Mean comparisons were performed by LSD and main effective interaction was found significant at P < 0.05.
Results and Discussion: 20 isolates of wheat rhizosphere bacteria were subjected to amplified ribosomal DNA restriction analysis. The 16S rDNA region was amplified by polymerase chain reaction and PCR products were digested by HpaII and RsaI restriction enzymes. From each pattern, one sample was sent to sequencing. Different species including; Chryseobacterium ginsenosidimutans, C. lathyri, C. piperi, C. taiwanense, Novosphingobium aromaticivorans, Pedobacter duraquae, and Sphingomonas koreensis were identified from the wheat rhizosphere. Bacteria were tested for their plant growth promoting qualities. All of the strains produced auxin from 1.90 to 25.93. Mean comparison of the data showed that the highest level of auxin was produced with F1 and the lowest amount was observed by F18. Phosphate solubilization measured as a halo zone on Sperber’s medium was observed with F6 and F56 isolates. The ratio of the diameter of the halo zone to the colony diameter was 2.86 with F6. The highest level of siderophore production by wheat rhizosphere bacteria, observed as halo formation around colonies on CAS Blue Agar medium, was obtained with F46, followed by F45 and F3. The ratio of the diameter of the orange halo surrounding bacterial growth to the colony diameter was 2.86 with F46. The result showed that the effect of wheat rhizosphere bacteria on germination traits such as radicle fresh and dry weight, plumule fresh weight, radicle and plumule length, germination percentage, germination rate, and germination average rate was significant at the one percent level and the effect of wheat rhizosphere bacteria on plumule fresh weight was significant at the five percent level.
Conclusion: Plant growth promoting bacteria enhance the growth and development of plants with different ways. These bacteria affect the growth and development of crops by phosphate solubilization, production of hydrogen cyanide, siderophore, and hormones such as auxin, gibberellic acid and cytokinins. According to the result, due to growth promoting characteristics such as siderophore and auxin production, phosphate solubilization, and the improvement of the seed germination traits, it can be possible to prepare bacterial inoculant for the field experiment in order to increase the availability of nutrients and improve the growth of plants.
H. Asadi Rahmani; A. Lakzian; J. Ghaderi; P. Keshavarz; H. Haghighatnia; K. Mirzashahi; M. R. Ramezanpour; A. Charati Arayi; A. Mohammadi Torkashvand
Abstract
Intoduction: Plant growth promoting rhizobacteria (PGPR) are a diverse group of bacteria consisting different species like Pseudomonas, Azotobacter, Azospirillum, Flavobacterium, Bacillus and Serratia with ability of enhancing plant growth and yield by different mechanisms. Flavobacteria are aerobic, ...
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Intoduction: Plant growth promoting rhizobacteria (PGPR) are a diverse group of bacteria consisting different species like Pseudomonas, Azotobacter, Azospirillum, Flavobacterium, Bacillus and Serratia with ability of enhancing plant growth and yield by different mechanisms. Flavobacteria are aerobic, gram negative, rod shape bacteria with more than 100 species living in different habitats ranging from soil and water to the foods. There are reports indicating that Flavobacteria are of dominant rhizosphere bacteria with beneficial effects on agricultural crops. Studies in Iran showed that six species of Flavobacterium were isolated and identified from rhizosphere of wheat. The aim of this study was to evaluate the effect of four strains of Flavobacterium on growth and yield of wheat under field conditions.
Materials and Methods: In this study four strains of Flavobacterium F9, F11, F21 and F40 were used. Bacterial strains were propagated in liquid NB growth medium and were used in field experiments. Fields were prepared in Khorasan Razavi, Khuzestan, Fars, Mazandran and Kermanshah and wheat seeds were inoculated with strains and sowed in a randomized complete block design (RCBD) with five treatments (four strains and a un-inoculated control) with four replications. Wheat varieties were Pishtaz in Khorasan and Fars, Marvdasht in Kermanshah, Chamran in Khuzestan and Milan in Mazandaran. Chemical fertilizers were used based on soil analysis. The rate of inoculation was 10 ml of bacteria per kg of seed. Plants were harvested at the end of the experiment and seed yield, total shoot biomass, 1000-seed weight, plant height, number of panicles per m2, number of seeds per panicle and panicle length were measured. Data analysis was performed by SPSS software, and the means were compared at α꞊5% by Duncan test.
Results and discussion: Results of the study showed that bacterial strains increased growth and yield of wheat in all provinces. In Mazandaran, all strains promoted seed yield although the effect of F21 was not significant. F40 had the highest effect on factors measures in the study. In Khuzestan, inoculation had no significant effect of seed yield production, although yield production was increased compared to control treatment. There was a similar trend regarding to other factors. In Khorasan, all factors were increased except for seed yield and 1000-seed weight due to inoculation with Flavobacterium strains. In Fars, inoculation with strain F40 significantly increased seed yield production by 11.5% compared to control treatment. In Kermanshah, seed yield, total biomass and plant height were significantly affected by inoculation with bacterial strains. Results showed that strain F40 was the most effective strain to increase yield of wheat. This study showed that Flavobacterium as a PGPR bacteria is able to positively affect the growth of wheat in Iran. This is in agreement with experiments in other parts of the world. In Khuzestan, bacteria were not effective on growth of wheat probably due to high soil temperature in this province compared to other provinces.
Conclusions: This study revealed that Flavobacteria are present in rhizosphere of wheat in Iran and could improve growth characteristics and yield of wheat in field experiments. Finally, strain F40 was the superior strain which increased seed yield by 15 % compared to control treatment.
M.A. Khalaj; F. Moshiri; Hadi Asadi Rahmani
Abstract
This study was conducted to evaluate N2-fixing ability of rhizobia strains inoculated with common bean. The experiment was carried out in a randomized complete block design (RCBD) with 13 treatments including 10 Rhizobium isolates, two nitrogen fertilizer levels (200 and 400 kg urea per ha) and one control ...
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This study was conducted to evaluate N2-fixing ability of rhizobia strains inoculated with common bean. The experiment was carried out in a randomized complete block design (RCBD) with 13 treatments including 10 Rhizobium isolates, two nitrogen fertilizer levels (200 and 400 kg urea per ha) and one control (without Nitrogen and rhizobium) in four replications at Esmaeel Abad research station of Qazvin. Plant sampling was done in two growth stages and nodule number and dry weight, shoot dry weight and total N-uptake and seed yield were evaluated. Results of two years field experiment showed that isolate L-75 showed the superior N2-fixing performance and had the highest positive effect on growth factors and could be recommended to use as inoculants strain in Qazvin region. This isolate, produced highest nodule number and dry weight in both years of study. Seed yield production was promoted by 26 and 40% more than control by application of isolate L-75 in the first and second year, respectively. This study showed that inoculation of common bean with effective rhizobia strains resulted in promoting of plant growth and seed yield production and have the benefit to reduce nitrogen fertilizer application in bean cropping.
A. Biari; A. Gholami; H. Asadi Rahmani
Abstract
Abstract
Soil beneficial microorganisms such as plant growth promoting rhizobacteria (PGPR) are able to enhance plant growth. The research was conducted using a randomized complete block design as split plot factorial with three factors (nutrient management, Azospirillum and Azotobacter strains) .In ...
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Abstract
Soil beneficial microorganisms such as plant growth promoting rhizobacteria (PGPR) are able to enhance plant growth. The research was conducted using a randomized complete block design as split plot factorial with three factors (nutrient management, Azospirillum and Azotobacter strains) .In this experiment the effect of Azospirillum and Azotobacter inoculation on yield and yield components of maize was evaluated in a field trial. Azotobacter levels were A0: no inoculation, A1: Azotobacter sp.Strain5 ، A2: Azotobacter chroococcum DSM2286 and Azospirillum levels were Z0: no inoculation, Z1: Azospirillum sp.Strain21, Z2: Azospirillum lipoferum DSM 1691, Z3:Azospirillum brasilense DSM1690. Results of this study showed inoculation with Azotobacter had significant effect on grain yield, stem and total dry weight, plant height and leaf area as well as 100 seed weight. The same results were obtained with Azospirillum inoculation. The interaction between two bacteria on grain yield, 100 seed weight, number of seed per ear, ear diameter, stem, leaf and total dry weight, plant height, leaf area and nitrogen uptake in seed were significant.
Keywords: Maize, PGPR, Azotobacter, Azospirillum, Yield
S. Rafiee; H. Asadi Rahmani
Abstract
Abstract
Among rhizospheric bacteria, great attention has been paid to the group of plant growth promoting rhizobacteria (PGPR), and their role in increasing the growth and health of plants. Therefore, it is used largely as inoculum all over the world. The rhizospheric bacterium, which has the genus ...
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Abstract
Among rhizospheric bacteria, great attention has been paid to the group of plant growth promoting rhizobacteria (PGPR), and their role in increasing the growth and health of plants. Therefore, it is used largely as inoculum all over the world. The rhizospheric bacterium, which has the genus of Flavobacterium, and promotes plants growth, has been studied in recent years. In the present research, 65 samples of rhizospheric soil were taken to isolate Flavobacterium from the rhizosphere of wheat cultivated in different regions of Iran. To isolate, cultivate, and preserve Flavobacterium, different and diverse formulations such as ATCC 647, 65 ATTC M1, and M1 Medium, which were recommended for this bacterium, were used for the development of a new specified culture media (FIM). Sixty-one isolates attributed to Flavobacterium were isolated and purified using specified culture medium (FIM). The genus and species were identified through microscopic, physiological and biochemical tests. The results obtained from our tests indicated that there are 5 species F. multivorum, F.odoratum, F. thalpophilum, F.balastinum and F. indoltheticum in the rhizosphere of wheat. Among the isolated species, F.odoratum showed the most frequency (72 percent), and the F. thalpophilum and F. balastinum and F. indoltheticum possessed the least frequency (1.6 percent).
Keywords: Flavobacterium, PGPR (Plant Growth Promoting Rhizobacteria), Rhizosphere, Wheat
