Soil science
B. Rezaei; A. Amirinejad; M. Ghobadi
Abstract
IntroductionIndustrial development has resulted in higher soils pollution with heavy metals. Plants which are subjected to heavy metals may severely lose their yield capabilities. Applying improving compounds in the soil is a new method to reduce effects of heavy metals on plants growth. Biochar as a ...
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IntroductionIndustrial development has resulted in higher soils pollution with heavy metals. Plants which are subjected to heavy metals may severely lose their yield capabilities. Applying improving compounds in the soil is a new method to reduce effects of heavy metals on plants growth. Biochar as a carbon rich source and salicylic acid as an important plant hormone, are two main compounds to alleviate heavy metals stresses in plants. These are the cost-effective and environmentally friendly substances for increasing the resistance of plants. Lead (Pb), as a common and extremely poisonous element in polluted soils, can be accumulated due to its non-biodegradability nature. When Pb content in plants reaches a toxic level, it can inhibit plant growth by reducing enzyme activities and photosynthesis and changing mineral nutrients balance. However, with regard to the program of expanding the area under cultivation of medicinal plants, including thyme, there is a possibility of contamination of soils in the vicinity of industrial centers and roads with lead. Therefore, the present study was conducted to evaluate the effects of salicylic acid as a plant growth stimulant and biochar made of rapeseed wastes, as a stable organic compound, on alleviation of Pb-induced stress in thyme (Thymus vulgaris L.). Materials and MethodsIn order to investigate the effects of salicylic acid (SA) and biochar (BC) on reducing Pb stress in thyme (Thymus vulgaris L.), a factorial experiment was conducted based on a completely randomized design with three replications in the greenhouse of Razi University. The factors included Pb at three levels (0, 150, and 300 mg/kg as Pb(NO3)2), SA at three levels (0, 150, and 300 μM) and BC at three levels (0, 1 and 3% by weight). To apply the Pb treatments, the soil samples of each pot (8 kg) were sprayed with Pb(NO3)2 solutions, 4 weeks before planting, according to the contamination levels. Then, BC treatments were performed by mixing it with the soil samples. In each pot, four thyme seedlings were planted. At four-leaf stage, SA solutions were sprayed three times on foliage of the thyme plants , until the beginning of flowering. After harvesting, some characteristics of aerial and root parts of thyme, including soluble sugars and proline contents, plant height, dry weights of shoots and roots, root volume and root length were determined. All plant parameters were then averaged for each pot. Also, Pb concentrations in extracts obtained from digestion of leaf tissues, were measured by Varian AA220 atomic absorption spectrophotometer. The analysis of variance (ANOVA) and comparison of means (Duncan's multiple range test) were performed using SPSS-16 software.Results and DiscussionThe results revealed that Pb stress reduced all plant characteristics, such as plant height, root volume and root length, as well as, dry weights of shoots and roots, and elevated leaf Pb concentration, proline content and soluble sugars in thyme. However, BC application resulted in improvements in growth parameters. The positive effect of BC was further enhanced when SA was sprayed onto the foliage of the thyme plants. The interaction effects of SA, BC and Pb treatments on the growth parameters of thyme, i.e, shoot dry weight, root volume, Pb concentration, soluble sugars and proline contents were significant (P < 0.01). In other words, SA and BC treatments moderated the negative effects of Pb on the growth traits. The highest Pb concentration (4.83 mg) and proline content (37.8 μmol/g) were obtained in 300 μg/kg of Pb, and SA and BC controls. Also, the highest concentration of soluble sugars (0.46 mg/kg) was found at 300 mg/kg of Pb, 300 μM SA and BC control.ConclusionOur results indicated the positive effects of SA and BC treatments on the growth parameters, such as; shoot and root dry weights in thyme plants, especially under Pb stress. In other words, Pb stress, while reducing all growth characteristics, increased proline content and soluble sugars in thyme. In general, it seems that under Pb stress, treatment of thyme with SA (as a plant growth regulator) and BC (as an organic matter with high viability in the soil) is a simple and appropriate method in order to increase the plant's resistance and reduce the effects of Pb toxicity on the overall growth of thyme.
M. Esmaeili; Bahman Farhadi Bansouleh; M. Ghobadi
Abstract
Introduction: Expansion of the area of oilseed crops such as soybean is one of the policies of Iranian agricultural policy makers as Iran is one of the major oilseed importers in the world. However, the area of this crop in Kermanshah province is negligible, but it could be cultivated in most parts ...
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Introduction: Expansion of the area of oilseed crops such as soybean is one of the policies of Iranian agricultural policy makers as Iran is one of the major oilseed importers in the world. However, the area of this crop in Kermanshah province is negligible, but it could be cultivated in most parts of this province. The quantity and quality of the produced grain could be affected by environmental factors such as weather parameters and water availability. The aim of the current study was to investigate the effects of levels of deficit irrigation on the quantity and quality of soybean crop yield in Kermanshah, Iran.
Materials and Methods: For this purpose, a field study was conducted as randomized complete block design with four replications and four irrigation treatments at the research farm of Razi University, Kermanshah in 2012. The size of each plot was 4 * 4 m. Irrigation treatments consisted of four irrigation levels: 20% over irrigation (T4), full irrigation (T3 as control), 20% less irrigation (T2) and 40% less irrigation (T1). The reason to choose T4 treatment was the lack of confidence in estimated crop evapotranspiration as there was no local calibration of crop coefficient (Kc) for this crop. The required water for T3 treatment was calculated based on daily weather data using FAO-Penman-Montith equation. Daily weather data was recorded in a weather station which was located in the research farm and is available in the www.fieldclimate.com. As there was no rainfall during the crop season, all of the required water was supplied through irrigation. The required water for treatments of T1, T2 and T4 was considered as 60%, 80% and 120% of T3 treatment. The required water was applied using a hose connected to a volumetric flow meter with a liter precision. Total amount of applied water during the crop season was 4399, 5865, 7331 and 8797 m3.ha-1 in the treatments. Fertilizers were applied based on the recommendations of soil fertility experts. Weeds were controlled manually. Finally, the area of two square meters in the middle of each plot was harvested in order to determine crop yield in terms of grain, biomass, stem, pod, seed protein content and fat percentage and also water productivity index. Dry weights of the samples were measured after drying samples in the oven for48hours at 70° C. The percentage of fat and protein in the grains are also measured in the laboratory. Water productivity index was calculated for each treatment by dividing crop yield (in terms of grain, biomass, protein and fat) over seasonal water use. Statistical analysis of the results is also done using MSTATC software.
Results and Discussion: The highest and lowest crop yields were measured respectively in the treatments T4 and T1.The mean value of grain yield was 1084, 1367, 1716 and 1940 kg.ha-1,respectively in the treatments T1, T2, T3 and T4. These results showed a 36% decrease in the grain yield by decreasing 40% in the amount of supplied water. However, biological yield was decreasedby the level of irrigation, but the rate of reduction was lower than that of grain yield. By reducing irrigation application, thepercentage of grain protein content increased while the percentage of fat in the grain decreased. Considering simultaneous reduction in grain yield and fat content in the grain, severe reductions in fat yield (oil content) were observed under water stress conditions. Crop yield in terms of fat was reduced by 26.2 and 50.1 %, respectively in treatments T2 and T1 in comparison with T3 (control treatment). The maximum and minimum percentages of protein in the treatments were 31% and 27%, respectively in the treatments T1 and T4. Maximum water productivity in terms of grain, biomass and protein was achieved in T1 treatment respectively with the amounts of 0.24, 0.81 and 0.077 kg.m-3. Maximum and minimum fat percentage was 0.052 and 0.040 kg.m-3, respectively in the T4 and T1 treatments. In addition,the results indicated that water productivity index in terms of grain, biomass and protein increased while they decreased in terms of fat yield.The results of statistical analysis indicated that water productivity index in all terms except protein had significant differences (at 5%) with T3 treatment.
Conclusion: Crop yield and water productivity (except in terms of fat) was increased by increasing applied water. Considering all indices of treatment T2 (20% deficit irrigation), itwas suggested as the best treatment.
