Soil science
Naghshineh Yari Nilavareh; Ali Beheshti Ale Agha; Mahin Karami; Marzieh Sadeghi
Abstract
IntroductionCrude oil is a complex combination of many hydrocarbon and non-hydrocarbon compounds, including heavy metals, which affect the physical and chemical properties of the soil, cause the soil particles to stick and connect and then cause the soil to become stiff and impenetrable. Contamination ...
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IntroductionCrude oil is a complex combination of many hydrocarbon and non-hydrocarbon compounds, including heavy metals, which affect the physical and chemical properties of the soil, cause the soil particles to stick and connect and then cause the soil to become stiff and impenetrable. Contamination of soil with petroleum hydrocarbons is a significant environmental problem, which has received remarkable attention in recent decades. Petroleum hydrocarbons are resistant and hazardous pollutants. Some petroleum hydrocarbons such as benzene are mutagenic and carcinogenic materials for humans. There are many physical and chemical methods to remediate oil-contaminated soils. Phytoremediation is a relatively new technology for refining contaminated soils in which resistant plants are used to remove or reduce the concentration of inorganic, radioactive, and organic pollutants, especially petroleum compounds, from the environment.Materials and MethodsSufficient amounts of about 50 kg of soil contaminated with petroleum hydrocarbons were collected from regions (0-30 cm soil depth) adjacent to the oil wells west of Kermanshah province. Uncontaminated soil samples were also taken from sites at the lowest distance to the contaminated sites. The aim of this study was to compare the efficiency of different plants to remove total petroleum hydrocarbons from oilfield soils. In this study, after determining the total amount of petroleum hydrocarbons, the contaminated and uncontaminated soils were mixed in 4 treatments with different weight ratios (0, 10, 25, and 35%). This experiment was established as completely randomized design with 3 replications for 6 different plants (Barley, Grass, Alfalfa, Hemp, Camelina, and Vicia ervilia). One treatment without plant was considered to remove soil matrix effects on petroleum hydrocarbon concentrations. Plants were harvested at the end of their growing season (90-120 days). Soils and plant samples from the experimental pots were analyzed for their important properties (including some physiological characteristics of the plants, as well as the percentage of reduced petroleum hydrocarbons in the soils). The gravimetric method was used to determine the concentration of petroleum hydrocarbons in the soil. After measuring the properties of the soil and plant, the normality of the data was checked by the Anderson–Darling test, and the homogeneity of the variance of the treatments was checked by using Levene's test. Analysis of data variance was done using ANOVA and average data comparison was done using LSD test at 5 and 1 percent probability levels (SAS 9.4 and SPSS 26).Results and DiscussionIn general, the growth of most plants showed a decreasing trend in proportion to the increase in soil pollution levels. However, the growth decline rates of different plants were not similar. Camelina was very sensitive to oil pollution and the plant could not tolerate pollution even at 10% level. After camelina, alfalfa was highly sensitive to oil pollution. The highest dry weight of the aerial parts of the hemp plant in the soil without oil contamination was observed at the rate of 111.22 grams in the pot. The leaf area of all studied plants in contaminated soils decreased compared to the control treatment (without contamination) so with the increase in the percentage of contamination, the leaf area of the plants was significantly reduced. The highest amount of leaf surface was observed in unpolluted soil and in the hemp plant. Except for the Camelina plant, which was completely destroyed at different levels of pollution, the rest of the plants showed a noticeable decrease in growth. The total petroleum hydrocarbons in soil were measured again 120 days after the start of cultivation, and its difference with the total amount of petroleum hydrocarbons at the beginning of cultivation was determined as the reduction of petroleum hydrocarbons and reported as a percentage. According to the mean comparison results, the percentage of reduced petroleum hydrocarbons was not significantly different among cultivated and non-cultivated treatments, although, it was significantly affected by soil pollution levels. Since all the studied soils contained natural bacteria and were not sterilized, the eliminated part of petroleum hydrocarbons is probably decomposed and removed by native bacteria in the soils. Therefore, the strengthening of native bacteria in these soils may increase the decomposition and degradation of petroleum hydrocarbons.ConclusionThe results of this research show that the presence of petroleum hydrocarbons in the soil caused a decrease in growth and other physiological characteristics in all studied plants. Although the Camelina was able to germinate in soils contaminated with petroleum hydrocarbons, the presence of these pollutants in the soil prevented the optimum growth of the plant, so its use in subsequent studies of phytoremediation of oil-contaminated soils, was not recommended. The results showed that there is no statistically significant difference between cultivated and non-cultivated treatments at different pollution levels, and the reduction of the total petroleum hydrocarbons in the soil was probably done by native microorganisms in the soil. It is recommended to take into consideration the efficiency of the plant species used, the type of polluting hydrocarbons, and the duration of contamination in future research to obtain better results.
Marjan Nekokhoo; Seyfollah Fallah; Rahim Barzegar
Abstract
Introduction: Water resources are very limited for agricultural production. Therefore, optimal use of available water resources and increased water use efficiency in agriculture are necessary. Application of poly ethylene mulch is one of the approaches that can be effective in increasing water use efficiency. ...
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Introduction: Water resources are very limited for agricultural production. Therefore, optimal use of available water resources and increased water use efficiency in agriculture are necessary. Application of poly ethylene mulch is one of the approaches that can be effective in increasing water use efficiency. The water deficient trend is increasing in agricultural lands of the Iran and, on the other hand, the yield components of hull-less seed pumpkin are sensitive to drought stress. Therefore, the aim of this study was to determine the effect of transparent polyethylene mulch on the performance and water use efficiency of hull-less seed pumpkin under different irrigation rates.
Materials and Methods: This experiment was carried out in the central part of Isfahan, Northern Baraan (320 and 32/N, 510 and 52/ E, and 1534 m above sea level) in a randomized complete block design with three replications, during 2016. Treatments consisted of full irrigation+ poly ethylene mulch (M+FW), 0.75% full irrigation+ poly ethylene mulch (M+0.75FW), 0.50% full irrigation+ poly ethylene mulch (M+0.5 FW), and full irrigation without mulch (FW). In April, the cultivation operations include mechanical planting, mulch were done. The spacing of the rows was 150 cm and the spacing between plants was 70 cm. The irrigation was applied until the plant was fully established and then drought stress was begun based on above irrigation treatments. The studied traits were number of fruits per plant, average fruit weight (kg), fruit yield (ton ha-1), number of seeds per fruit, fruit diameter (cm), 1000 grains weight (g), grain yield (kg ha-1), water use efficiency (kg m-3), oil content (%) and oil yield (kg ha-1). Statistical analysis was performed using SAS software and comparisons of the means were made using the least significant difference (LSD) test at the 5% probability level.
Results and Discussion: The highest number of fruits per plant belonged to M+FW treatment (with an average of 3.22) and the lowest was recorded in M+0.5FW treatment (with an average of 2.44). This difference between treatment FW and M+0.75FW can be due to the high moisture under the poly ethylene mulch. The highest fruit weight (3.60 kg) was obtained in M+FW treatments, which had a significant difference with other treatments. The difference weight of fruit in M + FW treatment was 14% compared to FW irrigation treatments. The highest fruit yield (95.72 ton ha-1) belonged to M+FW treatment and the lowest one (79.78) belonged to M+0.5FW treatment. The difference in fruit yield in M+0.75FW compared to FW treatment was 6%, but it was not significant. The number of seeds per fruit in M+0.75FW compared to FW and M+0.5FW treatments showed a difference of 13% and 17%, respectively which they were significant only with M+0.5FW treatment. With increasing drought stress, the amount of photosynthetic assimilate decreased, which reduced the number of seeds per fruit. The highest 1000 grains weight (173.13 g) belonged to M+FW treatment and the lowest one belonged to M+0.5FW (156.18 g). 1000 seeds weight in FW treatment was not significant compared to M+0.75FW treatment. Drought stress during plant development decreased the leaf area index in the plant. Application of plastic mulch reduces the effect of drought stress on leaf growth and its photosynthesis by decreasing water loss by evapotranspiration and transpiration. The difference grain yield between two treatments M+0.75FW with FW was 7% and this difference was not significant. Only significant difference was observed among M+0.5FW treatment with other treatments. The effect of different levels of moisture on water use efficiency was significant at 1% probability level. The highest water use efficiency was recorded in M+0.5FW treatment and the lowest was recorded in FW treatment. The difference in water use efficiency between M+0.75FW with full irrigation (FW) was 0.99 kg m-3, which was significant. Difference in water use efficiency between M+FW and FW was not significant for water use efficiency. The use of plastic mulch reduced water loss throughout the plant growth period and significantly increased water use efficiency. There was a significant difference among M+FW, M+0.75FW and FW for oil content. The highest and lowest oil contents belonged to M+FW and M+0.5FW, respectively. The maximum oil yield (558 kg ha-1) belonged to M+FW and the lowest one (412 kg ha-1) was obtained in M+0.5FW.
Conclusion: Transparent plastic mulch under water stress conditions can reduce the effect of drought stress on hull-less seed pumpkin by preserving water and other beneficial effects, including weeds reduction. Therefore, the use of this type of mulch is recommended for the cultivation of hull-less seed pumpkin in the central areas of the country facing the water crisis.
