جداسازی و ارزیابی ریزوباکتر‌های متحمل به سربِ محرک رشد گیاه از یک خاک حاوی سرب با سابقه بلند مدت

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه فردوسی مشهد

2 دانشگاه شهرکرد

چکیده

مطالعه‌ی حاضر به منظور جداسازی و ارزیابی ریزوباکتر‌های متحمل به سرب که قادر به تولید مواد محرک رشد گیاه تحت شرایط تنش فلزات سنگین بوده و توانایی افزایش زیست‌فراهمی فلز سنگین سرب را نیز دارند، به اجرا در آمد. ریزوباکتر‌های متحمل به سرب جدا شده از خاک آلوده به صورت Rhodococcus sp.، Bacillus stearothermophilus strain A، Corynebacterium sp.، Bacillus pumilus، Mycobacterium sp.، Bacillus stearothermophilus strain B، Bacillus licheniformis و Bacillus sp. جدا و شناسایی گردیدند. نتایج نشان داد که تمامی باکتری‌های جدا شده قادر به تحمل غلظت‌های بالای فلز سنگین سرب بودند. حداقل غلظت بازدارندگی سرب (MIC) این باکتری‌ها در دامنه‌ی 1100 تا 1720 میلی‌گرم بر لیتر (3/3 تا 19/5 میلی‌مولار) متغیر بود. به علاوه، تمامی سویه‌های باکتریایی جدا شده، از توانایی تولید ایندول استیک اسید (IAA) (از 5/3 تا 6/43 میلی‌گرم بر لیتر) و سیدروفور (از 7/57 تا 2/86 درصد) برخوردار بودند. با این وجود، تنها دو باکتری Mycobacterium sp. و Bacillus licheniformis توانایی تولید آنزیم ACC-دی‌آمیناز را داشتند. تلقیح محیط کشت حاوی کربنات سرب کم‌محلول با سویه‌ی باکتریایی Corynebacterium sp. به دلیل کاهش محسوس اسیدیته نیز سبب افزایش معنی‌دار غلظت سرب قابل‌جذب گردید.

کلیدواژه‌ها


عنوان مقاله [English]

Isolation and Evaluation of Lead (Pb)-Tolerant Plant Growth Promoting Rhizobacteria from a Soil Containing Pb for Long Term

نویسندگان [English]

  • M.R. Naderi 1
  • A. Danesh Shahraki 2
  • F. Raiesi 2
  • F. Nikookhah 2
1 Ferdowsi University of Mashhad
2 Shahrekord University
چکیده [English]

This study was performed in order to isolate lead (Pb)-tolerant plant growth promoting rhizobacteria in Pb-contaminated soils and to evaluate their potential for production of plant promoting substances. The isolated Pb-tolerant rhizobacteria were identified as Rhodococcus sp., Bacillus stearothermophilus strain A, Corynebacterium sp., Bacillus pumilus, Mycobacterium sp., Bacillus stearothermophilus strain B, Bacillus licheniformis and Bacillus sp. The results showed that all isolates were able to tolerate high concentrations of Pb. The minimum inhibitory concentration (MIC) of these bacteria was in the range of 1100-1720 mg l-1 (3.3-5.19 mM). In addition, all isolates produced IAA (ranging from 3.53 to 43.64 mg l-1) and siderophore (ranging from 57.74 to 86.24%). However, only two isolates (i.e., Bacillus licheniformis and Mycobacterium sp.) had the ability to produce bacterial enzyme ACC-deaminase. Inoculation of medium containing poorly soluble PbCO3 with bacterial strain Corynebacterium sp. significantly increased the available concentration of Pb.

کلیدواژه‌ها [English]

  • Pb-tolerant rhizobacteria
  • Minimum inhibitory concentration (MIC)
  • Indole acetic acid
  • Siderophore
  • ACC-deaminase
  • Lead carbonate solubilization
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