اثر برخی تنظیم کننده‌های رشد و سورفکتانت بر گیاه گلرنگ در خاک آلوده به کروم

نوع مقاله : مقالات پژوهشی

نویسندگان

1 شیراز

2 دانشگاه شیراز

چکیده

کاربرد تنظیم کننده­های رشد و سورفکتانت می­تواند روش مؤثری برای مقابله با تنش­هایی مانند آلودگی فلزات سنگین باشد. به منظور بررسی اثر تنظیم کننده­های رشد و سورفکتانت بر رشد و غلظت عناصر و شاخص­های گیاه­پالایی در گیاه گلرنگ، آزمایشی گلخانه­ای در قالب طرح کاملا تصادفی و به صورت فاکتوریل 3×4 شامل سه سطح سورفکتانت (شاهد، 5/2 و 5 میلی­مول در کیلوگرم) و چهار سطح تنظیم کننده رشد گیاه (شاهد، جیبرلیک اسید، ایندول استیک اسید و بنزیل آمینوپورین) با سه تکرار انجام شد. نتایج نشان داد اضافه کردن سورفکتانت و تنظیم کننده­های رشد باعث افزایش معنی­دار وزن خشک اندام هوایی، ضریب تجمع زیستی، شاخص جذب، غلظت و جذب کروم در اندام هوایی شدند. افزدون سورفکتانت باعث کاهش غلظت و جذب آهن در غیاب تنظیم کننده‌های رشد شد، اما در حضور تنظیم کننده‌های رشد گیاهی غلظت آهن افزایش می‌یابد. کاربرد 5 میلی­مول بر کیلوگرم سورفکتانت میانگین غلظت منگنز، مس و روی را کاهش داد. درحالی‌که اضافه کردن 5/2 میلی­مول بر کیلوگرم سورفکتانت غلظت فلزات را افزایش داد. اگرچه افزودن 5 میلی­مول بر کیلوگرم سورفکتانت وزن خشک را افزایش می­دهد اما اثر مطلوبی روی افزایش غلظت عناصر در گیاه ندارد. بنظر می­رسد که استفاده از تنظیم کننده­های رشد گیاه مقاومت گیاه را به سمیت کروم افزایش می­دهد که احتمالا از طریق افزایش جذب عناصر است. با توجه به نتایج استفاده از سورفکتانت به همراه تنظیم کننده­های رشد گیاه می­تواند علاوه بر افزایش توانایی گلرنگ در مقابله با سمیت کروم، گیاه­پالایی را نیز افزایش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of some Plant Growth Regulators and Surfactant on Safflower in Chromium Contaminated Soil

نویسنده [English]

  • samira keshavarz 1
1 shiraz
چکیده [English]

Introduction: Chromium (Cr) is one of the toxic metals widely used in leather tanning, alloy preparation, electroplating, drilling mud, refractory steel and catalytic manufacture. Besides the toxicity of chromium to human, it also disturbs the soil ecology and plant growth due to its toxic nature even at low concentration. Phytoremediation is effective and can be viewed as a relatively low cost, solar energy driven process for the management of contaminated soils. Hiwever the heavy metal toxicity adversely affects the plant growth and development. We can use some chemical compounds to increase plant resistance to heavy metal and increase the efficiency of phytoremediation. These days, foliar application of plant growth regulators such as Gibberelic acid Indole acetic acid and Benzyl amino purine are considered for various purposes such as enhancing plant growth and resistance to salinity, drought and heavy metals. Exogenous application of phytohormones can modulate the toxicity of Cr on plants most probably by maintaining hormonal balance of plant under metal stress. surfactants effectively enhance metal ion transfer to aqueous and hence increase their availability. Assessing surfactant assisted phytoremediation is important in order to ascertain the extent of its effectiveness under different conditions and to find its optimum level for metal phytoremediation. The application of plant growth regulators and surfactants can be an effective way to cope with stresses such as heavy metal contamination. The objectives of this study were to determine the effects of the growth hormones Gibberelic acid, Benzyl amino purine and Indole acetic acid alone and combined with surfactant on plant growth, concentration and uptake of Cr, Fe, Mn, Cu and Zn and some phytoremediation factor for Cr.
Material and Methods: The soil was air-dried and grounded to pass through a 2-mm sieve then was analyzed to determine various soil physic-chemical properties using standard methods. A greenhouse experiment was conducted in a completely randomized design with a factorial arrangement of 3 × 4 including three levels of surfactant (control, 2.5 and 5 mmol kg-1 soil) and four levels of plant growth regulators (control, Gibberellic acid, Indole acetic acid and Benzyl amino purine). All soils were contaminated by 5 mg/kg chromium and incubated for 1 month. During incubation, the soil samples were maintained at field capacity by distilled water. Safflower (Cartamus tinctorius L.) seeds were disinfested with 10% sodium hypochlorite, washed three time with distilled water and planted in the pots. Growth regulators were sprayed at three stage of 30, 20 and 40 days after planting at concentration of 1 mM. The plants were kept in the standard condition of greenhouse and the soil moisture content was maintained at field capacity by distilled water. Sixty days after planting, the plants were harvested and washed with distilled water, and then dried in oven at 65 Celsius until they reached a constant weight. Afterwards, the over-dried plant samples were grounded. Then dry ashing and extracting with 2 normal hydrochloric acid, the concentration of Cr, Fe, Zn, Cu and Mn in shoot was determined by atomic absorption (Shimadzu AA-670). The analysis of variance (ANOVA) was performed using a completely randomized design. Significantly different treatment means were separated using Duncan test (P < 0.05). Biological accumulation coefficient (BAC) and uptake index (UI) were calculated with a specific formula.
Results and Discussion: The results showed that addition of surfactant and growth regulators caused a significant increase in shoot dry weight, biological accumulation coefficient, uptake index and chromium concentration and uptake. Addition of surfactant reduced the concentration and uptake of iron in the absence of growth regulators, but in the presence of plant growth regulators, application of tween 80 increased iron concentrations. Application of 5 mmol kg-1of surfactant decreased mean concentration of manganese, copper and zinc. While addition of 2.5 mmol kg-1 of surfactant increased metals concentrations. Although addition of 5 mmol kg-1 surfactant increased dry weight, it did not have a satisfied effect on increasing the concentration of the elements in the plant. Plant growth regulators increased uptake of elements which is a protective mechanism against stresses.
Conclusion: It appears that using plant growth regulators increased the resistance of the plant to chromium toxicity probably through increasing absorption of the elements such as Fe, Mn, Cu and Zn. According to the results, application of tween 80 along with plant growth regulators could increase safflower capability to cope with chromium toxicity.

کلیدواژه‌ها [English]

  • Indole acetic acid
  • Benzyl amino purine
  • Tween 80
  • Gibberellic acid
  • Micro nutrient
- Adriano D.C. 2001. Trace elements in terrestrial environments: Biogeochemistry, bioavailability, and risks of metals, 2nd. In.: Springer-Verlag, New York.
- Afshan S., Ali S., Bharwana S.A., Rizwan M., Farid M., Abbas F., Ibrahim M., Mehmood M.A., and Abbasi G.H. 2015. Citric acid enhances the phytoextraction of chromium, plant growth, and photosynthesis by alleviating the oxidative damages in Brassica napus L. Environmental Science and Pollution Research 22: 11679-116789.
- Agnello A.C., Huguenot D., Van Hullebusch E.D., and Esposito G. 2016. Citric acid-and Tween80-assisted phytoremediation of a co-contaminated soil: alfalfa (Medicago sativa L.) performance and remediation potential. Environmental Science and Pollution Research International 23: 9215-9226.
- Akram N.A., Ashraf M., and Al-Qurainy F. 2012. Aminolevulinic acid-induced changes in some key physiological attributes and activities of antioxidant enzymes in sunflower (Helianthus annuus L.) plants under saline regimes. Scientia Horticulturae 142: 143-148.
- Almeida C.M.R., Dias A.C., Mucha A.P., Bordalo A.A., and Vasconcelos M.T.S. 2009. Influence of surfactants on the Cu phytoremediation potential of a salt marsh plant. Chemosphere 75: 135-140.
- Alvarez R., Nissen S.J., and Sutter E.G. 1989. Relationship between indole-3-acetic acid levels in apple (Malus pumila Mill) rootstocks cultured in vitro and adventitious root formation in the presence of indole-3-butyric acid. Plant Physiology 89: 439-443.
- Asilian E., Ghasemi-Fasaei R., Ronaghi A., Sepehri M., and Niazi A. 2018. Effects of microbial inoculations and surfactant levels on biologically-and chemically-assisted phytoremediation of lead-contaminated soil by maize (Zea Mays L.). Chemistry and Ecology 34: 964-977.
- Babaeian E., Homaee M., and Rahnemaie R. 2016. Chelate-enhanced phytoextraction and phytostabilization of lead-contaminated soils by carrot (Daucus carota). Archives of Agronomy and Soil Science 62: 339-358.
- Bohidar S., Thirunavoukkarasu M., and Rao T.V. 2008. Effect of Plant Growth Regulators on in vitro micropropagation of “Garden Rue”(Ruta graveolens L.). International Journal of Integrative Biology 3: 36-43.
- Boonyapookana B., Upatham E.S., Kruatrachue M., Pokethitiyook P., and Singhakaew S. 2002. Phytoaccumulation and phytotoxicity of cadmium and chromium in duckweed Wolffia globosa. International Journal of Phytoremediation 4: 87-100.
- Bouyoucos G.J. 1962. Hydrometer method improved for making particle size analyses of soils. Agronomy Journal 54: 464-465.
- Cabello-Conejo M.I., Centofanti T., Kidd P.S., Prieto-Fernandez Á., and Chaney R.L. 2013. Evaluation of plant growth regulators to increase nickel phytoextraction by Alyssum species. International Journal of Phytoremediation 15: 365-375.
- Cheng M., Zeng G., Huang D., Yang C., Lai C., Zhang C., and Liu Y. 2017. Advantages and challenges of Tween 80 surfactant-enhanced technologies for the remediation of soils contaminated with hydrophobic organic compounds. Chemical Engineering Journal 314: 98-113.
- Cserhati T. 1995. Alkyl ethoxylated and alkylphenol ethoxylated nonionic surfactants: interaction with bioactive compounds and biological effects. Environmental Health Perspectives 103: 358-364.
- Farid M., Ali S., Rizwan M., Ali Q., Abbas F., Bukhari S.A.H., Saeed R., and Wu L. 2017. Citric acid assisted phytoextraction of chromium by sunflower; morpho-physiological and biochemical alterations in plants. Ecotoxicology and Environmental Safety 145: 90-102.
- Farid M., Ali S., Rizwan M., Ali Q., Saeed R., Nasir T., Abbasi G.H., Rehmani M.I.A., Ata-Ul-Karim S.T., Bukhari S.A.H., and Ahmad T. 2018. Phyto-management of chromium contaminated soils through sunflower under exogenously applied 5-aminolevulinic acid. Ecotoxicology and Environmental Safety 151: 255-265.
- Farooq M.A., Ali S., Hameed A., Bharwana S.A., Rizwan M., Ishaque W., Farid M., Mahmood K., and Iqbal Z. 2016. Cadmium stress in cotton seedlings: physiological, photosynthesis and oxidative damages alleviated by glycinebetaine. South African Journal of Botany 104: 61-68.
- Fozia A., Muhammad A.Z., Muhammad A., and Zafar M.K. 2008. Effect of chromium on growth attributes in sunflower (Helianthus annuus L.). Journal of Environmental Sciences 20: 1475-1480.
- Gangwar S., Singh V.P., Srivastava P.K., and Maurya J.N. 2011. Modification of chromium (VI) phytotoxicity by exogenous gibberellic acid application in Pisum sativum (L.) seedlings. Acta Physiologiae Plantarum 33: 1385-1397.
- Halter L., Habegger R., and Schnitzler W.H. 2000. Gibberellic acid on artichokes (Cynara scolymus L.) cultivated in Germany to promote earliness and to increase productivity. In IV International Congress on Artichoke 681: 75-82.
- Houshm A., and Moraghebi F. 2011. Effect of mixed cadmium, copper, nickel and zinc on seed germination and seedling growth of safflower. African Journal of Agricultural Research 6: 1463-1468.
- Kaszycki P., Gabryś H., Appenroth K‐J., Jaglarz A., Sedziwy S., Walczak T., and Koloczek H. 2005. Exogenously applied sulphate as a tool to investigate transport and reduction of chromate in the duckweed Spirodela polyrhiza. Plant, Cell and Environment 28(2): 260-268.
- Kotb M.S. 2017. Effect of surfactant on adsorption and mobility of lead and cadmium in soils. Egypt Journal of Soil Science 57: 155-165.
- Krantev A., Yordanova R., Janda T., Szalai G., and Popova L. 2008. Treatment with salicylic acid decreases the effect of cadmium on photosynthesis in maize plants. Journal of Plant Physiology 165: 920-931.
- Lindsay W.L., and Norvell W.A. 1978. Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America Journal 42(3): 421-428.
- Liu D., Zou J., Wang M., and Jiang W. 2008. Hexavalent chromium uptake and its effects on mineral uptake, antioxidant defence system and photosynthesis in Amaranthus viridis L. Bioresource Technology 99: 2628-2636.
- Mouton J., Mercier G., and Blais J.F. 2009. Amphoteric surfactants for PAH and lead polluted-soil treatment using flotation. Water, Air, and Soil Pollution 197: 381-393.
- Naqvi S.S.M. 1999. Plant hormones and stress phenomena. Handbook of plant and crop stress, pp.709-730.
- Ramamurthy A.S., Vo D., Li X.J., and Qu J. 2008. Surfactant-enhanced removal of Cu (II) and Zn (II) from a contaminated sandy soil. Water, Air, and Soil Pollution 190: 197-207.
- Nelson D., and Sommers L.E. 1982. Total carbon, organic carbon, and organic matter. Methods of soil analysis. Part 2. Chemical and microbiological properties (methodsofsoilan2): 539-579.
- Rhoades J. 1996. Salinity: Electrical conductivity and total dissolved solids. Methods of Soil Analysis Part 3-Chemical Methods (methodsofsoilan3): 417-435.
- Saleem M., Asghar H.N., Khan M.Y., and Zahir Z.A. 2015. Gibberellic acid in combination with pressmud enhances the growth of sunflower and stabilizes chromium (VI)-contaminated soil. Environmental Science and Pollution Research 22: 10610-10617.
- Shafigh M., Ghasemi-Fasaei R., and Ronaghi A. 2016. Influence of plant growth regulators and humic acid on the phytoremediation of lead by maize in a Pb-polluted calcareous soil. Archives of Agronomy and Soil Science 62: 1733-1740.
- Solhi M., and Hajabbasi M.A. 2005. Heavy metals extraction potential of sunflower (Helianthus annuus) and canola (Brassica napus). Caspian Journal of Environmental Sciences 3: 35-42.
- Sujatha M., Geetha A., Sivakumar P., and Palanisamy N. 2008, November. Biotechnological interventions for genetic improvement of safflower. In Proceedings of the 7th international safflower conference (pp. 3-6).
- Swarnkar V., Agrawal N., and Tomar R. 2012. Sorption of chromate and arsenate by surfactant modified erionite (E-SMZ). Journal of Dispersion Science and Technology 33: 919-927.
- Tassi E., Pouget J., Petruzzelli G., and Barbafieri M. 2008. The effects of exogenous plant growth regulators in the phytoextraction of heavy metals. Chemosphere 71: 66-73.