Comparison of Soil Biological Activity in Forest with Coniferous and Broadleaf Trees in Biston Region of Kermanshah

Document Type : Research Article

Authors

• Sahar Mehrnoosh ¹
• Ali Beheshti Ale Agha ²
• Fatemeh Rakhsh ³
• Morteza Pourreza ⁴
• Ali Akbar Safari Sinegani ⁵

¹ MSc in Soil Biology, Department of Soil Science, Razi University, Kermanshah, Iran.

² Associate Professor, Department of Soil Science, Razi University, Kermanshah, Iran

³ Research Assistant, Department of Soil Science, Razi University, Kermanshah, Iran

⁴ Assistant Professor, Department of Natural Resources Engineering, Razi University, Kermanshah, Iran.

⁵ Professor, Department of Soil Science, Bu-Ali Sina University, Hamedan, Iran.

10.22067/jsw.2022.78164.1189

Abstract

Introduction
The maintenance of planted forests in arid and semi-arid lands is important. Soil formation in forest ecosystems is different with different tree species. Tree species have a direct and indirect effect on soil organisms. Forest ecosystems change their species composition and abundance of microorganisms, and consequently their biogeochemical cycles. The accumulation of vegetation biomass and the improvement of soil fertility can play a significant role in soil restoration.
Materials and Methods
In order to investigate the biological characteristics of the soil from 5 treatments, including agricultural (dry farming and relatively poor lands that are usually cultivated barley and wheat and have low productivity), pasture (pastures with minimal vegetation and high slopes that are affected by overgrazing have been changed to barren lands), forest with Acacia type (under and outside the crown), forest with the Cupressus arizonica type (under and outside the crown) and forest with the Pinus brutia type (under and outside the crown) randomly. Sampling was done in 3 repetitions from the 0 to 5 cm layer. The statistical sampling design of this research was completely random, in which, according to the type of afforested species, two types of coniferous forest stands (including Cupressus arizonica and Pinus brutia) and one broadleaf stand (Acacia species) were selected. Also, the area under the crown trees and outside the crown trees was also investigated. Soil samples were sampled with sterile equipment and crushed through a 4-mm sieve. Fresh and moist soil was kept at 4 °C temperature for soil biological tests. Microbial biomass carbon, soil basal respiration (197 days), substrate-induced respiration, and metabolic quotient were measured. Streptomycin sulfate was used to measure fungal respiration and cycloheximide was used to measure bacterial respiration. The activities of urease, acid, and alkaline phosphatase enzymes were determined. After measuring the biological properties of the soil, the normality of the data was checked by the Anderson–Darling test, and the homogeneity of the variance of the treatments was checked by using Levene's test. Analysis of data variance was done using One-Way ANOVA and average data comparison was done using Duncan's test at 5 and 1% probability levels (SAS 9.4 and SPSS 26).
Results and Discussion
The results of soil biological characteristics analysis showed that the highest values of soil respiration and amount of consumed organic matter, substrate-induced respiration, microbial biomass carbon, enzyme activities, and fungal respiration were measured in conifers. Although the amount of these features was also significant in broadleaf trees, they had significant differences. In this study, the high soil respiration rate in coniferous covers compared to broadleaf can be due to the high organic carbon content of the soil in this cover. According to the results of substrate-induced respiration in different coatings, likely the activity of microorganisms involved in the decomposition of organic matter in the studied habitats had a significant difference; Therefore, different coatings can affect the population of soil microorganisms as the main source of decomposition and emission of carbon dioxide by changing the quantity and quality of organic matter and other factors. Also, the highest values of metabolic quotient and bacterial respiration were observed in agricultural and pasture covers. A higher metabolic quotient in these covers indicates a decrease in the efficiency of the use of leaf litter by the soil microbial community. In general, the metabolic quotient in the bacterial community is higher than the fungal community; Therefore, it seems that the predominance of the bacterial population in agricultural and pasture cover has caused this index to increase, although plowing and cultivation, and disturbance of these covers have caused stress to this bacterial community and as a result increased the metabolic quotient deficit in these covers.
Conclusion
The results of this research showed that the type of planted tree species causes significant changes in the biological characteristics of the soil. The current research shows that the forest, whether coniferous or broadleaf, had the highest values of enzyme activities, basal respiration, substrate-induced respiration, microbial biomass carbon, and the lowest values of metabolic quotient compared to agricultural and pasture covers. Afforestation increases biological activity and possibly the number and diversity of microorganisms, and improves soil characteristics in the long term. In agriculture and pasture land, due to the destruction of soil and aggregates by agricultural activities such as plowing or excessive livestock grazing, the amount of organic carbon and the activity of microorganisms decreases, and with the decrease of other soil characteristics, the quality of the soil decreases over time. From this research, it can be concluded that the planting of forest species in the soils of degraded areas in the long term can increase soil organic carbon due to high-quality leaf litter, and as a result, increase permeability and soil moisture. Increasing soil organic carbon increases the activity of microorganisms, and in the long term, it will improve various soil characteristics. Planting forest plants in the natural areas of the country, which were destroyed due to the change of use to agriculture and indiscriminate cultivation and finally abandoned, can improve the characteristics of the soil and, as a result, establish the native vegetation of the region, and increase the permeability of water in the soil, the risk of soil erosion, floods, etc. reduce.

Keywords

• Bacterial respiration
• Enzyme activity
• Fungal respiration
• Microbial biomass
• Organic matter

20.1001.1.20084757.1401.36.5.5.5

Main Subjects

• Soil science

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References

1. Agusto, L., Jacqu, R., Binkely, D., & Rothe, A. (2002). Impact of European temperate forests on soil fertility. Annals of Forest Science 59: 233-253. https://doi.org/10.1051/forest:2002020.
2. Anderson, J.P.E., & Domsch, K.H. (1975). Measurement of bacterial and fungal contribution to the respiration of selected agricultural soils. Canadian Journal of Microbiology 21: 314–322.
3. Anderson, T.H., & Domsch, K.H. (1985). Maintenance carbon requirements of actively-metabolizing microbial populations under in situ conditions. Soil Biology and Biochemistry 17(2): 197-203. https://doi.org/10.1016/0038-0717(85)90115-4.
4. Avellaneda-Torres, L.M., Melgarejo, L.M., Narváez-Cuenca, C.E., & Sánchez, J. (2013). Enzymatic activities of potato crop soils subjected to conventional management and grassland soils. Journal of Soil Science and Plant Nutrition 13(2): 301-312. http://dx.doi.org/10.4067/S0718-95162013005000025.
5. Ayres, E., Steltzer, H., Berg, S., Wallenstein, M.D., Simmons, B.L., & Wall, D.H.) 2009). Tree species traits influence soil physical, chemical, and biological properties in high elevation forests. PLOS One 4(6): e5964.‏ https://doi.org/10.1371/journal.pone.0005964.
6. Bailey, V.L., Smith, J.L., & Bolton Jr, H. (2002). Fungal-to-bacterial ratios in soils investigated for enhanced C sequestration. Soil Biology and Biochemistry 34(7): 997-1007.‏ https://doi.org/10.1016/S0038-0717(02)00033-0.
7. Bakhshipour, R., Ramezanpour, H., & Lashkarboluki, E. (2012). Studying the effect of Pinus taeda and Populus plantation on some forest soils properties (Case study: Fidareh of Lahidjan). Iranian Journal of Forest 4(4): 321-332. (In Persian with English abstract)
8. Behera, N., & Sahani, U. (2003). Soil microbial biomass and activity in response to Eucalyptus plantation and natural regeneration on tropical soil. Forest Ecology and Management 174(1-3): 1-11. https://doi.org/10.1016/S0378-1127(02)00057-9.
9. Binkley, D. (2010). The influence of tree species on forest soils, processes and patterns. In: Proceedings of the trees and soil workshop. Mead, D.J., & Cornforth, I.S. (eds.). Agronomy Society of New Zealand Special Publication. Lincoln University Press, Canterbury.
10. Błońska, E., Lasota, J., & Zwydak, M. (2017). The relationship between soil properties, enzyme activity and land use. Forest Research Papers 78(1): 39-44.‏ https://depot.ceon.pl/handle/123456789/15059.
11. Cai, D., Yang, X., Wang, S., Chao, Y., Morel, J.L., & Qiu, R. (2017). Effects of dissolved organic matter derived from forest leaf litter on biodegradation of phenanthrene in aqueous phase. Journal of Hazardous Materials 324: 516-525. https://doi.org/10.1016/j.jhazmat.2016.11.020.
12. Chen, J., Wang, Q., Li, M., Liu, F., & Li, W. (2016). Does the different photosynthetic pathway of plants affect soil respiration in a subtropical wetland. Ecology and Evolution6(22): 8010-8017.‏ https://doi.org/10.1002/ece3.2523.
13. Deharveng, L. (2011). Soil collembola diversity, endemism and reforestation: A case study in the Pyrenees (France). Conservation Biology 10: 74-84. https://doi.org/10.1046/j.1523-1739.1996.10010074.x.
14. Ghorbanzadeh, N., Pourbabaei, H., Salehi, A., Soltani Tolarood, A.A., & Alavi, S.J. (2018). Investigation of the microbial and soil invertebrates biodiversity indices of hard wood and soft wood plantations in west of Guilan province. Applied Soil Research 6(3): 1-12. (In Persian with English abstract)
15. Gianfreda, L., & Bollag, M.J. (1996). Influence of Natural and Anthropogenic Factors on Enzyme Activity in Soil. Soil Biochemistry 9: 123-193.
16. Hölscher, D., Hertel, D., Leuschner, C., & Hottkowitz, M. (2002). Tree species diversity and soil patchiness in a temperate broad-leaved forest with limited rooting space. Flora-Morphology. Distribution, Functional Ecology of Plants 197(2): 118-125. https://doi.org/10.1078/0367-2530-00021.
17. Hur, J., Park, M.H., & Schlautman, M.A. (2009). Microbial transformation of dissolved leaf litter organic matter and its effects on selected organic matter operational descriptors. Environmental Science and Technology 43(7): 2315-2321. https://doi.org/10.1021/es802773b.
18. Iovieno, P., Alfani, A., & Bååth, E. (2010). Soil microbial community structure and biomass as affected by Pinus pinea plantation in two Mediterranean areas. Applied Soil Ecology 45(1): 56-63. 1016/j.apsoil.2010.02.001.
19. Islam, K.R., & Weil, R.R. (2000). Land use effects on soil quality in a tropical forest ecosystem of Bangladesh. Agriculture, Ecosystems and Environment 79: 9–16. https://doi.org/10.1016/S0167-8809(99)00145-0.
20. Jones, D.L., Cooledge, E.C., Hoyle, F.C., Griffiths, R.I., & Murphy, D.V. (2019). PH and exchangeable aluminum are major regulators of microbial energy flow and carbon use efficiency in soil microbial communities. Soil Biology and Biochemistry 138: 107584. https://doi.org/10.1016/j.soilbio.2019.107584.
21. Kandeler, E., & Gerber, H. (1988). Short-term assay of soil urease activity using colorimetric determination of ammonium. Biology and Fertility of Soils 6(1): 68-72. https://doi.org/10.1007/BF00257924.
22. Khormali, F., & Shamsi, S. (2009). Micromorphology and quality attributes of the loess derived soils affected by land use change: a case study in Ghapan watershed, northern Iran. Journal of Mountain Science 6: 197-204. https://doi.org/10.1007/s11629-009-1037-z.
23. Kiani, F., Jalalian, A., Pashaee, A., & Khademi, H. (2004). Effect of deforestation on selected soil quality attributes in loess-derived land forms of Golestan Province, northern Iran. Proceedings of the Fourth International Iran and Russia Conference: 546-550.
24. Kooch, Y. (2012). Soil variability related to pit and mound, canopy cover and individual trees in a Hyrcanian Oriental Beech stand. Ph.D. Thesis, Tarbiat Modares University, 203p. (In Persian with English abstract)
25. Kooch, Y., & Parsapoor, M.K. (2016). The effects of broad and needle-leaved forest covers on soil microbial indices. Journal of Water and Soil Conservation 23(2): 195-210. https://doi.org/22069/jwfst.2016.3063.
26. Kooch, Y., & Zoghi, Z. (2014). Comparison of soil fertility of Acer insigne, Quercus castaneifolia and Pinus brutia stands in the Hyrcanian forests of Iran. Chine. Journal of Applied Environmental and Biological Sciences 20: 899-905. https://doi.org/3724/SP.J.1145.2014.02011.
27. Leirós, C., Trasar-Cepeda, C., Seoane, S., & Gil-Sotres, F. (2000). Biochemical properties of acid soils under climax vegetation (Atlantic Oakwood) in an area of the European temperate–humid zone (Galicia, NW Spain): general parameters. Soil Biology and Biochemistry 32: 733-745. https://doi.org/10.1016/S0038-0717(99)00195-9.
28. Mallik, A.U., & Hu, D. (1997). Soil respiration following site preparation treatments in boreal mixedwood forest. Forest Ecology and Management 97: 265–275. https://doi.org/10.1016/S0378-1127(97)00067-4.
29. Mu, Z., Huang, A., Ni, J., & Xie, D. (2014). Linking annual N₂O emission in organic soils to mineral nitrogen input as estimated by heterotrophic respiration and soil C/N ratio. PlOs One 9(5): e96572. https://doi.org/10.1371/journal.pone.0096572.
30. Nannipieri, P., & Alef, K. (1995). Methods in Applied Soil Microbiology and Biochemistry. Netherlands: Elsevier Science.
31. Neatrour, M.A., Jones, R.H., & Golladay, S.W. (2005). Correlations between soil nutrients availability and fine- root biomass at two spatial scales in forested wetlands with contrasting hydrological regimes. Canadian Journal of Forest Research 35(12): 2934-2941. https://doi.org/10.1139/x05-217.
32. Polyak, Y.M., & Sukcharevich, V.I. (2019). Allelopathic interactions between plants and microorganisms in soil ecosystems. Biology Bulletin Reviews 9(6): 562-574. https://doi.org/1134/S2079086419060033.
33. Rasouli-Sadaghiani, M.H., Barin, M., Siavash Moghaddam, S., Damalas, C.A., & Ghodrat, K. (2018). Soil quality of an Iranian forest ecosystem after conversion to various types of land use. Environmental Monitoring and Assessment8: 447. https://doi.org/10.1007/s10661-018-6815-z.
34. Salehi, A., Matinizadeh, M., & Tamjidi, J. (2012). Investigation on effect of forest plantation of Alnus ghutinosa (Gaertn.) and Pinus taeda L. on soil microbial activity and biomass (case study: Geisom site, west of Guilan province, Iran). Iranian Journal of Forest and Poplar Research 20(2): 345-334. (In Persian with English abstract). https://doi.org/10.22092/ijfpr.2012.107304.
35. Sheikhloo, F., & Rasouli Sadaghiani, M. (2016). Effects of different agronomic and forest land uses on soil enzyme activity. Iranian Journal of Soil and Water Research 47(1): 205-216. (In Persian with English abstract). https://doi.org/22059/ijswr.2016.57992.
36. Silva, E.D., de Medeiros, E.V., Duda, P., Lira, M.A., de Oliveira, J.B., dos Santos, U.J., & Hammecker, C. (2019). Seasonal effect of land use type on soil absolute and specific enzyme activities in a Brazilian semi-arid region. Catena 172: 397-407. https://doi.org/10.1016/j.catena.2018.09.007.
37. Singh, R., Bhardwaj, D.R., Pala, N.A., Kaushal, R., & Rajput, B.S. (2018). Soil microbial characteristics in sub-tropical agro-ecosystems of North Western Himalaya. Current Science 115: 1956-1959. https://www.jstor.org/stable/26978529.
38. Soleimani, A., Hosseini, S.M., Bavani, A.R.M., Jafari, M., & Francaviglia, R. (2019). Influence of land use and land cover change on soil organic carbon and microbial activity in the forests of northern Iran. Catena 177: 227-237. https://doi.org/10.1016/j.catena.2019.02.018.
39. Susyan, E.A., Ananyeva, N.D., & Blagodatskaya, E.V. (2005). The antibiotic-aided distinguishing of fungal and bacterial substrate-induced respiration in various soil ecosystems. Microbiology74(3): 336-342.‏ https://doi.org/10.1007/s11021-005-0072-1.
40. Tabatabai, M.A., & Bremner, J.M. (1969). Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry 1(4): 301-307. https://doi.org/10.1016/0038-0717(69)90012-1.
41. Ushio, M., Kitayama, K., & Balser, C. (2010). Tree species effects on soil enzyme activities through effects on soil physicochemical and microbial properties in a tropical montane forest on Mt. Kinabalu, Borneo. Pedobiologia 53: 227-233. https://doi.org/10.1016/j.pedobi.2009.12.003.
42. Wani, F.S., Akhter, F., Mir, S., Baba, Z.A., Maqbool, S., Zargar, M.Y., & Nabi, S.U. (2018). Assessment of soil microbial status under different land use systems in North Western Zone of Kashmir. International Journal of Current Microbiology and Applied Sciences 7: 266-279. https://doi.org/10.20546/ijcmas.2018.708.032.
43. Wardle, D., Bonner, K., & Barker, G. (2002). Linkages between plant litter decomposition, litter quality, and vegetation responses to herbivores. Functional Ecology 16: 585–595. https://doi.org/10.1046/j.1365-2435.2002.00659.x.
44. Yadava, R. (2012). Soil organic carbon and soil microbial biomass as affected by restoration measures after 26 years of restoration in mined areas of Doon Valley. International Journal of Environmental Sciences 2: 1380-1385.
45. Yang, N., Ji, L., Yang, Y., & Yang, L. (2018). The influence of tree species on soil properties and microbial communities following afforestation of abandoned land in northeast China. European Journal of Soil Biology 85: 73-78. https://doi.org/1016/j.ejsobi.2018.01.003.
46. Yao, H., He, Z.L., Wilson, M., & Campbell, C.D. (2000). Microbial biomass and community structure in a sequence of soils with increasing fertility and changing land use. Microbial Ecology 40(3): 223-237. https://doi.org/10.1007/s002480000053.
47. Zavaleta, E., & Hulvey, K. (2007). Realistic variation in species composition affects grassland production, resource use and invasion resistance. Plant Ecology 188: 39–51. https://doi.org/10.1007/s11258-006-9146-z.
48. Zeng, D.H., Hu, Y.L., Chang, S.X., & Fan, Z.P. (2009). Land cover change effects on soil chemical and biological properties after planting Mongolian pine (Pinus sylvestris mongolica) in sandy lands in Kerning, northeastern China. Plant and Soil 317: 121-133. https://doi.org/10.1007/s11104-008-9793-z.
49. Zeng, Z., Wang, S., Zhang, C., Tang, H., Li, X., Wu, Z., & Luo, J. (2015). Soil microbial activity and nutrients of evergreen broad-leaf forests in mid-subtropical region of China. Journal of Forestry Research 26(3): 673-678.‏ https://doi.org/10.1007/s11676-015-0060-x.