J. Khallizadeh; E. Dordipour; M. Baranimotlgh; A. Gharanjiki
Abstract
Introduction: Iron deficiency is one of the most important nutritional disorders in plants, particularly in calcareous soils and deeply affects the yield and quality of the product. Due to the major role of iron in the synthesis of chlorophyll, chlorosis occurs in young leaves in deficiency conditions. ...
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Introduction: Iron deficiency is one of the most important nutritional disorders in plants, particularly in calcareous soils and deeply affects the yield and quality of the product. Due to the major role of iron in the synthesis of chlorophyll, chlorosis occurs in young leaves in deficiency conditions. In such condition, biochar can help to increase OM, soil fertility level, and iron use efficiency and, to reduce iron chlorosis. The aim of this study was to investigate the effect of iron- impregnated biochar on the availability of iron and the elimination of soybean iron chlorosis in a calcareous soil.
Materials and Methods: Calcareous soil with iron deficiency (0-30 cm) was collected from the east of Golestan province and prepared for cultivation. Two types of biochar were produced from wheat straw and particleboard through slow pyrolysis (increasing 5 °C/min) at 300 °C for 2 hours under restricted oxygen conditions in an electric furnace, and then impregnated with iron sulfate solution. FTIR spectra and SEM images of biochars surfaces were also provided. A pot experiment was conducted as a factorial based on a completely randomized design with four replications. Factors were biochars (wheat straw biochar (WB) and particleboard biochars (PB) each one with 2.5% w/w), iron impregnated biochars (Fe impregnated wheat straw biochar 2.5% w/w (Fe- IWB1) and 5% w/w (Fe-IWB2), 2.5% w/w (Fe-IPB1) and 5% w/w (Fe-IPB2) Fe impregnated particleboards, Fe- Sequestrene (S) and control without Fe and biochar (C), and two soybean cultivars (Williams and Saman). The sown pots were maintained near the field capacity for 12 weeks. Then, SPAD numbers, concentration and uptake of active iron in young and senile leaves and active iron content in soil were determined after harvest.
Results and Discussion: With increasing application of iron impregnated biochar, active iron content increased in the soil. SPAD numbers of the upper leaves of both soybean cultivars in Fe impregnated biochars were significantly higher than those of non-impregnated biochars and control treatments (P ≤ 0.05). Iron chlorosis symptoms in soybeans decreased following the increased application of Fe impregnated biochars, consequently, there were no iron chlorosis symptoms in 5% Fe impregnated biochar treatments. Also, the active iron concentration of the upper leaves and the amount of leaf active iron uptake significantly increased as a result of Fe impregnated biochars application in both soybean cultivars compared to control and non-impregnated biochars (P ≤ 0.05). The highest concentration of active iron in upper leaves was observed in 5% w/w Fe impregnated biochars treatments, but its value for cultivar Williams in Fe impregnated wheat biochar was higher than that in Fe impregnated particleboard biochar. The results of the SEM images indicated that wheat biochar had more quantity of and fine pores (also CEC) than that of the particleboard biochar, and the surface areas of both biochars were rough and dark after impregnation with iron, indicating the adsorption or accumulation of iron at their surfaces. Also, there was a significant positive correlation between the active iron concentration with SPAD numbers in the upper leaves (r = 0.88 **) and dry weight of soybean shoots (r = 0.87 **). Cultivars responses to Fe impregnated biochars showed that iron uptake and active iron concentration in the upper leaves of Williams variety were significantly less than those of Saman variety at both levels of Fe impregnated biochars (P ≤ 0.05), which indicates that cultivar Williams is more susceptible to the iron chlorosis. The results of this experiment and reports from other studies show that the application of impregnated biochars from nutrients besides increasing SOM, permeability and soil moisture, CEC and soil fertility level, also increases the acquisition and use efficiency of iron in the plant.
Conclusion: The results of this study showed that due to the strong adsorption of soil iron, non-impregnated biochar application in the level of 2.5% had no significant effect on the concentration and uptake of active iron and spad numbers of the plant. However, using Fe impregnated biochar and increasing their application in calcareous soils with iron chlorosis resulted in a significant increase of active soil iron content, concentration and uptake of active iron and SPAD numbers of the plant, and, conversely, a decrease of leaf chlorosis. Therefore, besides improving the physical, chemical and biological properties of the soil, the application of Fe impregnated biochar can also be a promising approach to eliminate iron chlorosis in sensitive plants, particularly soybeans in calcareous soils.
A. Jahandideh; M. Barani; E. Dordipour; R. Ghorbani Nasrabadi
Abstract
Introduction: One of the most important needs in the farm planning is the evaluation of different systems of plant nutrition. By supplying the correct way of plant nutrition, one can preserve the environment and increase the efficiency of agricultural inputs. Humic acid contains many nutrients that increase ...
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Introduction: One of the most important needs in the farm planning is the evaluation of different systems of plant nutrition. By supplying the correct way of plant nutrition, one can preserve the environment and increase the efficiency of agricultural inputs. Humic acid contains many nutrients that increase soil fertility, soil organic matter content, and access to macro- and micro-nutrients by preventing the formation of insoluble salts and chelating properties. Phosphorus and humic acid stimulate vegetative growth, improve reproductive growth, and increase the quantitative and qualitative yield of plants. In this regard, the positive effects depend on the amount and how they are applied. The present study was conducted with the aim of investigating the effects of different levels of humic acid and phosphorus fertilizer on phosphorus availability and photosynthetic pigments (a, b and carotenoids) in canola (cv. Hyola 50).
Methods and Materials: The soil used in this study was collected from 0-30 cm layer of a soil profile passed through a 2-mm sieve after air-drying. The soil chemical and physical properties were then determined. The pot experiment was conducted as factorial based on completely randomized design with three replications. Treatments include phosphorous fertilizer as super phosphate in three levels (0, 50 and 100 mg/kg) and humic acid in three levels (0. 0.5 and 1 gr/kg soil), phosphorous and humic acid application ways. Humic acid and phosphorous treatments were mixed in various forms including simultaneous mixing of humic acid and phosphorous fertilizer in the soil matrix, application of humic acid and phosphorous via irrigation water and coting of phosphorous fertilizer via solid humic acid before soil application. Then 10 canola seeds were planted in each pot at 2-cm depth which were declined to 4 plants in each pot after emerging and greening phases. At the end of the growth period (158 days), the plants were harvested. Determination of phosphorus concentrations of plant extracts by molybdenum vanadate or yellow method and chlorophyll content (a, b and ab) and carotenoids were measured precisely before harvesting using Barnes method. After harvesting the plants, the soil was immediately air-dried and passed through a 2mm sieve. Then, the amount of phosphorus was determined by sodium-DTPA and sodium bicarbonate. The statistical results of the data were analyzed using SAS software and LSD test (at 5% level) was used for comparing the mean values.
Results and Discussion: The interactions of humic acid and phosphorus and its application methods were significant for all measured traits at the 5% level. The results of the triple effects of humic acid levels and its application at the presence of phosphorus treatments showed that the highest chlorophyll (a, b and ab) and carotenoid content was obtained at 100 mg/kg phosphorus and 1 g/kg humic acid along with irrigation water. The highest concentration of plant shoot phosphorus with an average of 0.30% was observed in 1 g/kg humic acid with irrigation water at the level of 100 mg/kg phosphorus, although had no significant difference with 0.5 g/kg of humic acid with irrigation water. Maximum amount of P was extracted by Olsen method with the mean of 16.14 mg/kg and Soltanpour and Schwab method with the mean of 5.24 mg/kg obtained in 100 mg/kg phosphorus and 1 g/kg soil humic acid application. There was a significant correlation between the phosphorus extracted by Olsen method and Soltanpour and Schwab method (r = 0.95), which was significantly correlated with concentration of phosphorus (r = 0.84) and (r = 0.85) (P<0.05). There was also a significant correlation between fresh and dry above-ground biomass, types of chlorophyll (a, b and ab) and carotenoids with phosphorus extracted by Olsen and Soltanpour and Schwab methods at 5% significance level.
Conclusion: P adsorption capacity is a function of many factors. Application of phosphorous fertilizers in calcareous soils, due to the presence of calcium with high activity, results in the formation of calcium phosphates, which becomes insoluble, over time. Humic material in interaction with phosphorus in the soil can reduce phosphorus stabilization and increase plant available phosphorus. The results of this study showed that the use of phosphorus with humic acid, rather than the use of phosphorus alone, could increase the available phosphorus in the soil and also the phosphorus concentration within the plant.
Taleb Nazari; mojtaba barani; Esmaeil dordipour; Reza Ghorbani nasrabadi; Somayeh Sefidgar shahkolaie
Abstract
Introduction: Fe is the first identified micronutrient for crops and required in higher amount than other micronutrients. Fe plays important roles in enzyme metabolism, protein metabolism, chlorophyll construction, chloroplast evolution, photosynthesis, respiration and reduction-oxidation reaction as ...
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Introduction: Fe is the first identified micronutrient for crops and required in higher amount than other micronutrients. Fe plays important roles in enzyme metabolism, protein metabolism, chlorophyll construction, chloroplast evolution, photosynthesis, respiration and reduction-oxidation reaction as well as organic acids metabolism. Iron, as an essential micronutrient, has great contribution in important antioxidant enzymes activity and through which affects plant tolerance against environmental stresses. Plant enzymes including superoxide dismutase, catalase and glutathione peroxidase are among the most important enzymes scavenging the hydrogen peroxide have iron in their structure, so they affected by iron deficiency. In this study, the effect of soil, foliar and fertigation application of humic acid on iron availability, chlorophyll types and superoxide dismutase, catalase and glutathione peroxidase enzymes in canola (Hyola 308) were evaluated.
Results and Discussion: Results showed that highest total iron content in plant leaves was obtained in 0.4 percent foliar application and the lowest was belonged to control treatment. Highest iron content in plant stem and active iron was obtained in humic acid application through irrigation at 2000 mg L-1 by 85 and 44.86 mg kg-1, respectively, and lowest amounts were obtained in control by 54.62 and 20.40 mg kg-1. Also, greatest concentration of chlorophyll a, chlorophyll b and total chlorophyll were recorded under0.4 percent humic acid foliar application by 3.58, 1.79 and 5.37 and the lowest chlorophyll contents were associated to control. Highest activities for plant enzymes superoxide dismutase and glutathione peroxidase were obtained under0.1 percent foliar application of humic acid by 4.20 and 1.95 (Iu/gr. FW) and the highest activity for catalase enzyme by 4.46 Iu/gr FW in 1000 mg L-1 humic acid through was irrigation and the lowest enzyme activity obtained in control treatment. Findings showed that application of various levels of humic acid increased plant enzyme activity compared to control in all of three application method (soil, foliar and application through irrigation water). Increasing humic acid concentration decreased enzyme activities. Also, there was negative correlation between activity of plant enzymes and concentation of chlorophyll types and active iron.
Conclusions: Active iron and antioxidant enzymes represent iron status within cell cytoplasm. Based on the results of this study, active iron concentration and activity of antioxidant enzymes are appropriate indices for evaluating plant tolerance to iron deficiency compared to assessing total iron content in leaves.
Somayeh Sefidgar; Mojtaba Barani Motlagh; farhad khormali; Esmael Dordipour
Abstract
Introduction: Soil pollution with heavy metals have become a global concern because of its damaging effects on the environment, including human health, toxicity in plants and long-term effects on soil fertility. Heavy metals stress in plants is characterized by decrease in photosynthesis, nutrient uptake, ...
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Introduction: Soil pollution with heavy metals have become a global concern because of its damaging effects on the environment, including human health, toxicity in plants and long-term effects on soil fertility. Heavy metals stress in plants is characterized by decrease in photosynthesis, nutrient uptake, damaging of roots and finally plant death. Lead (Pb) is found to be the most dangerous heavy metal, responsible for reduced soil fertility and elevated environmental pollution. Lead toxicity causes the inhibition of seed germination and exerts adverse effects on growth and metabolic processes of plants, which retards plant and crop production. The overproduction of reactive oxygen species (ROS) is the best indicator for secondary stress, which results in a number of toxic effects on biochemical processes in many plant cells. The overproduction of ROS due to Pb stress brings about changes in cellular membrane permeability, which in turn damages organelles such as nuclei, mitochondria, and chloroplasts in plant cells which decreased plant growth and yield. Chemical stabilization is an in situ remediation method that uses inexpensive amendments to reduce contaminant availability in polluted soil. The aim of this study was to investigate the immobilization of lead in a calcareous contaminated soil using two types of biochar as organic and Pumice, Leca, Zeolite and Bentonite as inorganic amendments.
Materials and Methods: In order to investigate the effect of organic amendments (biochar 640°C, and biochar 420°C) and inorganic amendments (Pumice, Leca, Zeolite and Bentonite) on Pb stabilization in a contaminated soil (1500 mg/kg), a greenhouse experiment using maize plant was carried out. This experiment was conducted in a completely randomized design consisting of 6 types of amendments (Pumice, Leca, Zeolite, Bentonite, Biochar 420°C, and Biochar 640°C) and at 1% and 5% levels of each amendment (12 amendments plus 1 control). The experimental treatments were incubated for 3 months. At the end of incubation time, the potential bioavailability of Pb in non-amended and amended soils was assessed by chemical extractions, as: extraction with DTPA, with ammonium acetate and with ethylenediaminetetraacetic acid (EDTA). After the end of incubation time, the pots were transferred to a greenhouse and in each pot five maize seeds were planted and then reduced to three seedlings in each pot after germination. After 3 months, all the plants were harvested. The Pb concentration in each plant, its biomass, its chlorophyll and its antioxidant enzyme activities levels were analyzed. All statistical analyses were performed using SAS software. Means of different treatments were compared using LSD (P ≤0.05) test.
Results and Discussion: The results indicated that the addition of amendments to soils reduced the concentration of Pb extracted with DTPA and EDTA. The 5% biochar 640 had the greatest reduction effect on DTPA-extractable Pb. The smallest concentration of Pb in the leaves and root of maize plant was observed in treated soil with organic amendments (biochar 640°C, and biochar 420°C) and treated with 5% zeolite, respectively. The highest increase in plant growth parameters like SPAD value, leaf area, plant height, number of leaves per plant, dry biomass yield and dry matter of roots were observed in organic amendments compared to the control. The application of 5% amendments in soil caused a significant increase in plant height and number of leaves as compared to control. The increase in growth and biomass of zea mays L. under various amendments might be due to decreased bioavailable Pb concentrations in soil amended which may be attributed to reduced Pb toxicity through improvement of soil fertility. Also, the application of amendments resulted in a significant increase in antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), peroxidase (PX), and ascorbate peroxidase (ASP) in maize plants compared to the control. The increase of leaves enzyme activities with addition amendments may be due to a lower Pb accumulation in leaves because excess Pb generates free radicals and reactive oxygen species (ROS) those causes oxidative stress in plants.
Conclusions: The results indicated that the application of amendments were successful in lowering the potential bioavailability of Pb in the soils. The 5% biochar 640 treatment had the greatest reduction effect on extractable Pb. The application of amendments decreased the uptake and accumulation of Pb in maize plants, via the reduction of DTPA- extractable Pb. The amendments also significantly increased leaves antioxidant enzyme activities and photosynthetic pigments compared to the control.
Z. Naghizadeh Asl; E. Dordipour; A.L. Gholizadeh; F. Kiani; H. Emami
Abstract
Abstract
In order to assess the availability of phosphorous (P), different methods have been proposed in the last decades. The results of these methods in soils with various pH, calcium carbonate, organic matter, Al and Fe oxides and hydroxides and other properties especially in Iranian soils can not ...
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Abstract
In order to assess the availability of phosphorous (P), different methods have been proposed in the last decades. The results of these methods in soils with various pH, calcium carbonate, organic matter, Al and Fe oxides and hydroxides and other properties especially in Iranian soils can not be used. In addition, knowledge about transformation of P forms is necessary for perception of P behavior. The objective of this research was to study the relationship between P extracted by some extract ants and soil properties and, different forms of inorganic P. Therefore 20 soil samples were collected from 0-30 cm depth of Glosetan province soils. Soil P was extracted and measured by sodium bicarbonate at pH = 8.5 (NaHCO3), DTPA- Ammonium bicarbonate (NH4HCO3-DTPA), DTPA- Sodium bicarbonate (NaHCO3-DTPA), and 0.01 M CaCl2. Also, different forms of inorganic P in the studied soils were measured by Jiang and Gu sequential fractionation method. The results showed that the amount of P extracted by different extract ants was in the order NaHCO3 > NaHCO3-DTPA > NH4HCO3-DTPA > CaCl2. There was a significant correlation between P extracted by different extract ants and determination coefficient between P extracted by NaHCO3 and NaHCO3-DTPA, NaHCO3 and NH4HCO3-DTPA, and NaHCO3-DTPA and NH4HCO3-DTPA were 0.91**, 0.85**, and 0.82** respectively. P extracted by NaHCO3, NH4HCO3-DTPA, and NaHCO3-DTPA with pH, EC, clay and Calcium carbonate percentage had a negative and significant correlation, but with Organic matter it was significant and positive. Also among the P forms only di, octa and Al oxides P had a significant correlation with P extracted by studied extract ants. In general the distribution of inorganic P forms in these soils was as below: Ca10-P > Al-P > Ca8-P > Fe-P > Ca2-P > Fe- Occluded-P. According to significant correlation between P extracted by studied extract ants with some P forms it seems that, some P forms can be transferred to available form of plants.
Keywords: P Extract ants, Sequential fractionation, Inorganic P forms
