Soil science
H. Asgari; M. Barani Motlagh; S.A. Movahedi Naeini; A. Babaei
Abstract
Introduction
Wheat is considered the most important grain and one of the vital food products in Iran. After nitrogen, phosphorus is the most important nutrient required by plants and holds a high priority for the growth, yield and quality of plants. However, due to the introduction of phosphorus in ...
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Introduction
Wheat is considered the most important grain and one of the vital food products in Iran. After nitrogen, phosphorus is the most important nutrient required by plants and holds a high priority for the growth, yield and quality of plants. However, due to the introduction of phosphorus in various reactions in the soil, a small amount of consumed phosphorus fertilizer is removed by the plant and the rest of it is left in a non-absorbable form in the soil. The efficiency of using phosphorus fertilizers and the availability of this nutrient is considered as a limiting factor for the production of agricultural products in calcareous soils with alkaline reaction of Iran. Since graphene and its oxidized form, with large amounts of active oxygen groups and high specific surface area, have been proposed by many studies as non-toxic and biocompatible materials in the production of compounds with improved efficiency of using nutrient, therefore to increase the efficiency of phosphorus consumption in soil, in this study, phosphorus was loaded on graphene oxide (GO-P). The present study aims to assess the influence of this compound as a source of phosphorus and its mixing with triple superphosphate fertilizer (GO-P-TSP) compared to triple superphosphate soluble fertilizer (TSP) on the amount of water retention of fertilizers in soil and phosphorus concentration in aerial parts of wheat plant.
Methods and Materials
Graphene oxide was prepared based on the modified Hamers method. Then graphene oxide was adjusted to certain pH and iron sulfate as a source of iron ions was added to the graphene oxide suspension with vigorous stirring. The mixture was stirred for one hour and then centrifuged for 30 minutes. Then the supernatant was removed and the residue of the compound was dry frozen. In the next step, pH was adjusted with sodium hydroxide (NaOH) solution. Then a certain weight of potassium dihydrogen phosphate salt (KH2PO4) was added to the above suspension. The mixture was stirred for one hour and centrifuged for 30 minutes. After centrifugation, the supernatant was removed and the remains of the phosphorus composition based on graphene oxide were dry frozen. Loading tests were performed in three replicates. pH, EC, bulk density, total concentration of phosphorus and iron and X-ray diffraction spectroscopy (EDS) analysis were measured in the sample of phosphorus composition based on graphene oxide. Then three fertilizer formulations were selected, which included (1) triple superphosphate fertilizer, (2) synthesized phosphorus fertilizer based on graphene oxide, and (3) mixing graphene oxide-phosphorus compound with triple superphosphate fertilizer in a ratio of 50:50% phosphorus.
To investigate the water retention behavior of fertilizers in the soil, dried samples of the three studied fertilizer formulations was added into a sandy soil completely and weighed. At the same time, dried sandy soil without fertilizer was placed in another beaker as a control. Then each beaker was added distilled water and weighed. The beakers were weighed once every three days at room temperature until they reached constant mass. The water-retention behavior of the soil was calculated.
In order to investigate the effect of three fertilizer formulations on phosphorus availability, soil with low amount of phosphorus was selected and physical and chemical properties of the soil sample were measured at a depth of 0-30 cm. A greenhouse experiment on wheat planting was conducted using a randomized complete design with 3 replications. The treatments included three fertilizer formulations at three fertilization levels (10, 15, and 20 mg kg-1) with 3 replications. The control treatment was performed without phosphorus fertilizer. Plants were harvested 72 days after planting, washed with distilled water and dry with tissue paper. The samples were air-dried and then oven dried at 70˚C to a constant weight in a forced air-driven oven. After harvesting, the weight of fresh and dry matter and phosphorus concentration in the soil and aerial parts of the plant were measured. Statistical data were analysed using SAS software (9.4) and the mean values were compared using LSD tests (at 1 and 5% level).
Results and Discussion
The composition of phosphorus based on graphene oxide (GO-P) in powder form had 35.5% of total P2O5, 31.1% of soluble in water P2O5, 19.6 of total iron and 15.28% of total potassium. The result of EDS analysis confirmed the loading of phosphorus on graphene oxide. The pH of the phosphorus composition based on graphene oxide was 5.8, approximately 2.5 units higher than triple superphosphate fertilizer. The bulk density of the compound (GO-P) was significantly lower than triple superphosphate fertilizer. The EC of the compound (GO-P) was similar to the EC of the triple superphosphate fertilizer. Soil water retention with synthesized phosphorus fertilizer based on graphene oxide (GO-P) was higher than soil (control) and other compounds added to soil. Experimental results showed that the addition of prepared fertilizer formulas (GO-P and GO-P-TSP) increased water retention in the soil for a longer period of time, while in the soil without adding fertilizer and triple superphosphate treatment, respectively, from 10 and 11 days, the absorbed water completely evaporated. Therefore, the combination of soil with GO-P and GO-P-TSP compared to the soil without fertilizer and the combination of soil with triple super phosphate (TSP) fertilizer had better water retention behavior. The greenhouse experiment results of wheat planting showed that all treatments were significant (P<0.01). Among all the treatments and measured levels, the control treatment showed the lowest value. The highest concentration of phosphorus in aerial parts of wheat (0.31%) and in soil after harvesting (9.5 mg kg-1), fresh (10.6 g per pot) and dry weight (2.03 g per pot) of aerial wheat plants were related to the treatment of phosphorus compounds based on graphene oxide at the level of 20 mg kg-1.
Conclusion
The highest concentration of phosphorus in aerial parts of wheat was related to the treatment of phosphorus compound based on graphene oxide at the level of 20 mg kg-1. Therefore, with more research in the future to produce "nutritious plants" in sustainable, efficient and flexible agricultural systems, we can benefit from technologies based on carbon materials.
Soil science
T. Nazari; M. Barani Motlagh; S.O. Rastegar; M.H. Sedri
Abstract
IntroductionPhosphorus is an essential element for all living organisms, and it cannot be replaced by any other element. Phosphorus has however a limited resource, and it is estimated that the extracted phosphorus resources (Apatite) will last for another 50 to 100 years. One of the most widely used ...
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IntroductionPhosphorus is an essential element for all living organisms, and it cannot be replaced by any other element. Phosphorus has however a limited resource, and it is estimated that the extracted phosphorus resources (Apatite) will last for another 50 to 100 years. One of the most widely used technologies for recycling phosphorus is the precipitation of phosphorus from sewage sludge and leachate. Phosphorus Recovery as struvite (NH4MgPO4.6H2O) from sewage sludge has attracted special attention due to its potential for use as an ecological and slow release fertilizer. Struvite is a white, grain-like solid, odor-free and sludge–free ingredient, composed of magnesium, ammonium and phosphate at equal molar concentrations. Therefore, this study is designed to examine the effect of struvite replacement with triple superphosphate fertilizer on some physiological parameters and phosphorus availability in wheat plants in calcareous soils deficient in phosphorus. Methods and MaterialsSoil with phosphorus deficiency was collected from 0-30 cm depth under arable lands of Hajjiabad-e Seyyedeh located in Ghorveh township, Kurdistan Province, Iran. The soil was air-dried and ground to pass through a 2-mm sieve, followed by laboratory analysis to determine its physico-chemical properties. The struvite used in the research was obtained by optimizing the three main factors of sulfuric acid concentration, solid-to-liquid ratio, and time for the leaching process, and the three key factors of Mg:P ratio, N:P ratio and pH for the precipitation process by Response Surface Methodology. To achieve the aim of this study a factorial experiment was carried based on completely randomized design with 4 replications. The factors included the application of different proportions of struvite replaced with triple superphosphate (S0:P100, S25:P75, S50:P50, S75:P25 and S100:P0) and 4 levels of phosphorus (0, 50, 100 and 150 kg TSP ha-1) and a total of 54 pots. The application rate for struvite was calculated based on total phosphorus (P2O5). Then 10 wheat seeds were planted in each pot at 2-cm depth which after plant emerging and greening, declined to 4 plants in each pot. The pots were randomly moved twice a week during the growth period to eliminate environmental effects. Irrigation and weeding operations were applied by hand. Plants were harvested 60 days after planting (beginning of flowering), washed with distilled water and dried with tissue paper. The samples were air-dried and then oven dried at 70˚C to a constant weight in a forced air-driven oven. Phosphorus concentrations in plant extracts were measured by the molybdenum vanadate or yellow method and chlorophyll content (a, b and ab) and carotenoids using the Arnon method. The statistical results of the data were analyzed using SAS software and LSD test (at 5% level) was used for comparing the mean values. Results and DiscussionBased on the obtained results, all the investigated treatments and their interactions were significant at p<0.01. However, the interaction effect of fresh weight shoots and height was significant at p<0.05. The comparison of the average data showed that the highest amount of fresh weight shoots (7.79 g pot-1), dry weight shoots (1.130 g pot-1) and height (29.66 cm) was obtained from the application of S75:P25 150 kgTSP ha-1. By use of struvite instead of triple superphosphate fertilizer, the phosphorus concentration and uptake of wheat increased at all three fertilizer levels, so that the highest phosphorus concentration (0.174%) was obtained from S75:P25 150 kg TSP ha-1. However, there was no statistically significant difference for S100:P0 (0.169%) treatment. The highest amount of phosphorus uptake in wheat with an average of 0.197 g pot-1 was obtained from the S75:P25 treatment (150 kg TSP ha-1), compared to the treatment of 100% struvite (S100:P0) and 100% triple superphosphate fertilizer (S0:P100) with the averages of 0.158 and 0.109 g pot-1, respectively, showing 19.79 and 44.67 percent increase. Also, the results showed that the treatment of 150 kg TSP ha-1 100% struvite (S100:P0) compared to 100% triple superphosphate fertilizer (S0:P100) increased the amounts of chlorophyll a, b, ab and carotenoids by 7.78, 3.82, 6.44 and 6.84 percent, respectively. ConclusionDespite struvite's low solubility, it is a highly soluble phosphorus fertilizer for plants . However, the reasons for this apparent contradiction and also the specific mechanisms of struvite dissolution are still unclear. Hence, further accurate measurements at different pH and EC conditions with different physical and chemical properties of soil studying phosphorus fractionation in soil will help to better understand the use of struvite. Therefore, it is recommended to optimize the timing and application rate of struvite in relation to the demand for different agricultural and garden crops.
Soil science
K. Asadi; M. Barani Motlagh; S.A. Movahedi Naein; T. Nazari
Abstract
Introduction Methods and MaterialsThis experiment was carried out in a field near the village of Takhshi Mahalle, located 5 km northwest of Gorgan city with geographical coordinates (54° 17´ 56 ʺ N) (52° 51´ 36 ʺ E) in 2022. The physical and chemical properties of the soil ...
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Introduction Methods and MaterialsThis experiment was carried out in a field near the village of Takhshi Mahalle, located 5 km northwest of Gorgan city with geographical coordinates (54° 17´ 56 ʺ N) (52° 51´ 36 ʺ E) in 2022. The physical and chemical properties of the soil were measured at a depth of 0-30 cm in different parts of the farm and the final composite soil was analyzed in the laboratory. Water was measured using conventional methods of sampling and testing water and wastewater. The experiment was conducted as a randomized complete block design with 3 replications. The treatments included control (with distilled water), foliar spraying of iron sulfate micronutrient elements [FeSO4.7H2O (20%Fe)], zinc sulfate [ZnSO4.7H2O (22% Zn)], and iron sulfate + zinc sulfate at a concentration of 5 per thousand at the 4-leaf stage, the 8-leaf stage and both stages (4-leaf and 8-leaf). Foliar spraying was done in the early morning and drip irrigation was used. Plants were harvested 120 days after planting, washed with distilled water and dried with tissue paper. The samples were air-dried and then oven dried at 70˚C to a constant weight in a forced air-driven oven. Iron and zinc concentrations were determined by an atomic absorption device. In order to determine the protein percentage and yield in different treatments, total nitrogen was measured by the Kjeldahl method. The protein percentage and yield were calculated using the following formula: Statistical data were analysed using SAS software (9.4) and the mean values were compared using LSD tests (at 5% level). Results and DiscussionThe obtained results showed that all treatments effects were significant (P<0.01) (fresh forage P<0.05). Among all the treatments and measured traits, the control treatment showed the lowest value. The highest iron concentration with an average of 175.14 mg kg-1 was obtained using iron foliar spraying in both 8 and 4 leaf stages, which increased 22.73 and 34.39% in comparison with only using iron foliar application in 4 and 8 leaf stages, respectively. Zinc foliar spraying at both the 4 and 8 leaf stages resulted in the highest zinc concentration of 71.02 mg kg-1 in forage corn, increasing zinc concentration by 89.86% over the control. In both 4 and 8 leaf stages, an iron and zinc foliar application had the highest chlorophyll index with an average of 57.63. The highest nitrogen content, averaging 2.80%, was observed following foliar spraying of iron and zinc during both the 4 and 8 leaf stages. This represents an increase of 5% and 23.92% compared to iron and zinc foliar application treatments during the respective stages. Consequently, the highest yield and protein percentage were also attained, averaging 310.75 grams per square meter and 17.50%, respectively, with simultaneous foliar application of iron and zinc during both the 4 and 8 leaf stages. ConclusionThe optimal outcomes for measured traits were observed when iron and zinc were concurrently applied at both the 4 and 8 leaf stages. Therefore, it is advisable to administer iron and zinc simultaneously during these growth stages to ensure the attainment of forage with desirable quantitative and qualitative characteristics.
Soil science
M. Bazi Abdoli; M. Barani Motlagh; A. Bostani; T. Nazari
Abstract
IntroductionOrganic matter and alkaline pH are the main causes of nutrient deficiencies in calcareous soils of arid and semi-arid regions. The availability of some nutritional elements, including the micronutrients such as iron, zinc, copper, and manganese is very low in calcareous soils, although the ...
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IntroductionOrganic matter and alkaline pH are the main causes of nutrient deficiencies in calcareous soils of arid and semi-arid regions. The availability of some nutritional elements, including the micronutrients such as iron, zinc, copper, and manganese is very low in calcareous soils, although the total concentration of these elements may be relatively high. Burning crop residues results in substantial loss of nutrients, and may lead to air pollution and human health problems. An alternative approach is to apply crop residues to soil in the form of biochar. The biochar modification with acid may increase the solubility of nutrients (P, Fe, Zn, Cu, Mn) present in biochar, thereby significant improvement in mineral nutrition of plants grown in calcareous soils. Therefore, the object of this study is to investigate the effect of acid-modified biochar from rice residues on the amount of chlorophyll and the micronutrient concentration of quinoa plant (Chenopodium quinoa) in a calcareous soil. Methods and MaterialsThe soil was air-dried and ground to pass through a 2-mm sieve then was analyzed to determine various soil physico-chemical properties using standard methods. To achieve the aim of this study the factorial experiment was carried out based on a completely randomized design in 4 replications. Factors include 3 types of biochar (unmodified, modified by pre-acidic method and modified by post-acidic method) and different levels of biochar (0, 2, and 5% by weight). Then 10 quinoa seeds were planted in each pot at 2-cm depth which after emergence, declined to 3 plants in each pot. The pots were randomly moved twice a week during the growth period to eliminate environmental effects. Irrigation and weeding operations were performed by hand. Determination of chlorophyll content (a, b, and ab) and carotenoids were measured precisely before harvesting in fresh plants using Arnon method. Plants were harvested at 187 days after planting, washed with distilled water and dry with tissue paper. The samples were air-dried and then oven dried at 65˚C to a constant weight in a forced air-driven oven. Then the total micronutrient content of the plant was determined after dry ashing. The statistical results of the data were analyzed using SAS software (9.4) and LSD test (at 5% level) was used for comparing the mean values. Results and DiscussionBased on the variance analysis, all attributes responded positively to different types and levels of biochar and modified biochar (p<0.01). The comparison of the average effect of the studied treatments showed that with the increase in the levels of all three types of biochar, the amount of chlorophyll a, b, total, and carotenoid increased so the highest amount of chlorophyll a, b, total, and carotenoid respectively, with an average of 2.58 and 1.54, 4.13 and 1.36 mg g-1 were obtained from the treatment of 5% post-acidic biochar. The results showed that the highest amount of Fe concentration in shoots with an average of 229.48 mg kg-1 was obtained from the treatment of 5% post-acidic biochar, although there was no statistically significant difference with the treatment of 5% pre-acidic biochar with an average of 220.48 mg kg-1 and its lowest value with an average of 95.95 mg kg-1 was related to unmodified biochar. The highest amount of Zn concentration in shoots with an average of 13.42 mg kg-1 was related to the treatment of 5% post-acidic biochar which showed an increase of 13.24 and 33.26% compared to the treatment of 5% pre-acidic and unmodified biochar, respectively. Also, the highest concentrations of Cu and Mn in shoots were obtained with an average of 3.85 and 23.37 mg kg-1 respectively, from the treatment of 5% post-acidic biochar. ConclusionPost-acidic biochar had better results in terms of physiological indices and the concentration of micronutrients (Fe, Zn, Cu, and Mn) than unmodified biochar in quinoa. The increase of nutrients in quinoa can be attributed to the dissolution of biochar nutrients after being modified with acid and the reduction of pH and the availability of these elements in the soil. Therefore, biochar modified with acid or biochar produced from sources that have acidic properties can be recommended as a suitable method for improving fertility and increasing micronutrients in calcareous soils affected by salt.
Soil science
E. Mirparizi; M. Barani Motlagh; S.A. Movahedi Naeini; R. Ghorbani Nasrabadi; S. Bakhtiary
Abstract
Introduction: Iron deficiency is one of the most common nutritional problems of plants in arid and semi-arid soils especially in calcareous soils. Iron is essential to many cellular activities, required for optimum growth and development, however it is insoluble in aerated soils at neutral or basic pH, ...
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Introduction: Iron deficiency is one of the most common nutritional problems of plants in arid and semi-arid soils especially in calcareous soils. Iron is essential to many cellular activities, required for optimum growth and development, however it is insoluble in aerated soils at neutral or basic pH, therefore, iron deficiency is common in these soils. The problem is usually solved by using iron synthetic chelates which is a very expensive option. There is, therefore, a need for cheaper and more effective alternatives to traditional Fe fertilizers. Several reports have shown that application of Fe factory by-product to soil tends to raise the availability of Fe and reduce Fe deficiency in plants. Application of organic compounds to soil may improve the solubility of the minerals containing micronutrients and correct their deficiencies in alkaline and calcareous soils. A large amount of slag is produced annually at the Sarcheshmeh Copper Complex, Kerman Province, Iran. So far, the copper slag, however, has not been tested as an Fe fertilizer in calcareous soils. Since about 53.8% of slag obtained from copper concentrate melting is composed of iron oxides, we, thus, examined the effect of copper slag along with organic compounds on the level of upper leaf iron, photosynthetic pigments, SPAD index, the activity of plant enzymes and the level of active iron in the upper leaves of sorghum by performing a factorial experiment in a completely randomized design.Materials and Methods: In order to study the effect of copper slag (one of by-products of melting copper concentrate in Sarcheshmeh Copper Complex, Kerman Province) and organic compounds (cow manure and pistachio skin) on total leaf iron content, photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll), antioxidant enzymes activity (Guiacol Peroxidase, Glutathione peroxidase) and concentration of active iron of young leaves developed of sorghum, a pot experiment was conducted in the greenhouse with three replicates per treatment. We applied experimental treatments including 5 levels of organic matter (pistachio skin, cow manure at 2 and 4 wt. % and control sample), and 11 levels of iron (copper slag, copper slag with sulfur, copper slag with sulfur and thiobacillus, acidic slag (each 2 levels each), sequesterine, foliar application of EDTA, and control sample) to a soil sample with low iron content. At the end of the incubation period, sorghum bicolor was cultured in the above treatments. Ten seeds were sown in each pot. Seedlings were thinned to 4 when they were about 10 cm high. During the growth period, pots were irrigated with distilled water as needed. Before harvesting, SPAD, the concentration of photosynthetic pigments, level of active iron and activity of plant enzymes were also measured in fresh plant samples. Furthermore, the concentration of Fe in the leaves was measured. Analysis of variance was performed using software SAS and significant differences were determined based on LSD (Least Significant Difference Test) at p < 0.05 level.Results and Discussion: The interaction between slag treatments and organic compounds showed that treatments of 4 wt. % of cow manure with slag of 4 times of recommended soil test value (C4S4, and 4 wt. % of cow manure with slag of 4 times of recommended soil test value with sulfur and thiobacillus (C4S4S°T), had significant effects on increasing photosynthetic pigments pigments (chlorophyll a, chlorophyll b, total chlorophyll), SPAD index, activity of plant enzymes (Guiacol peroxidase, Glutathione peroxidase) and active iron. The highest active iron level in the young leaves developed (54.06 mg / kg) was observed in (C4S4S°T) treatment which showed a significant increase compared to the control treatment (17.14). Increased concentration of active iron was also observed due to application of treatments (slag, organic compounds and the interaction between treatments) in sorghum young leaves. The photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll), SPAD index and activity of plant enzymes (Guiacol peroxidase, Glutathione peroxidase) were more correlated with active iron in young leaves developed compared to total iron concentration in these leaves. This indicates that active iron can be used as an index to detect iron deficiency.Conclusion: The higher level of active iron in the young leaves developed was more associated with physiological indices of sorghum as compared with total Fe concentration in these leaves. Therefore, this parameter can be used as an index to detect iron deficiency. In this study, increasing the level of slag consumed and consequently increasing Fe concentration in the leaf resulted in a significant increase in chlorophyll a, chlorophyll b, total chlorophyll, carotenoids and activation of the plant enzymes.
J. Khallizadeh; E. Dordipour; M. Baranimotlgh; A. Gharanjiki
Abstract
Introduction: Iron deficiency is one of the most important nutritional disorders in plants, particularly in calcareous soils and deeply affects the yield and quality of the product. Due to the major role of iron in the synthesis of chlorophyll, chlorosis occurs in young leaves in deficiency conditions. ...
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Introduction: Iron deficiency is one of the most important nutritional disorders in plants, particularly in calcareous soils and deeply affects the yield and quality of the product. Due to the major role of iron in the synthesis of chlorophyll, chlorosis occurs in young leaves in deficiency conditions. In such condition, biochar can help to increase OM, soil fertility level, and iron use efficiency and, to reduce iron chlorosis. The aim of this study was to investigate the effect of iron- impregnated biochar on the availability of iron and the elimination of soybean iron chlorosis in a calcareous soil.
Materials and Methods: Calcareous soil with iron deficiency (0-30 cm) was collected from the east of Golestan province and prepared for cultivation. Two types of biochar were produced from wheat straw and particleboard through slow pyrolysis (increasing 5 °C/min) at 300 °C for 2 hours under restricted oxygen conditions in an electric furnace, and then impregnated with iron sulfate solution. FTIR spectra and SEM images of biochars surfaces were also provided. A pot experiment was conducted as a factorial based on a completely randomized design with four replications. Factors were biochars (wheat straw biochar (WB) and particleboard biochars (PB) each one with 2.5% w/w), iron impregnated biochars (Fe impregnated wheat straw biochar 2.5% w/w (Fe- IWB1) and 5% w/w (Fe-IWB2), 2.5% w/w (Fe-IPB1) and 5% w/w (Fe-IPB2) Fe impregnated particleboards, Fe- Sequestrene (S) and control without Fe and biochar (C), and two soybean cultivars (Williams and Saman). The sown pots were maintained near the field capacity for 12 weeks. Then, SPAD numbers, concentration and uptake of active iron in young and senile leaves and active iron content in soil were determined after harvest.
Results and Discussion: With increasing application of iron impregnated biochar, active iron content increased in the soil. SPAD numbers of the upper leaves of both soybean cultivars in Fe impregnated biochars were significantly higher than those of non-impregnated biochars and control treatments (P ≤ 0.05). Iron chlorosis symptoms in soybeans decreased following the increased application of Fe impregnated biochars, consequently, there were no iron chlorosis symptoms in 5% Fe impregnated biochar treatments. Also, the active iron concentration of the upper leaves and the amount of leaf active iron uptake significantly increased as a result of Fe impregnated biochars application in both soybean cultivars compared to control and non-impregnated biochars (P ≤ 0.05). The highest concentration of active iron in upper leaves was observed in 5% w/w Fe impregnated biochars treatments, but its value for cultivar Williams in Fe impregnated wheat biochar was higher than that in Fe impregnated particleboard biochar. The results of the SEM images indicated that wheat biochar had more quantity of and fine pores (also CEC) than that of the particleboard biochar, and the surface areas of both biochars were rough and dark after impregnation with iron, indicating the adsorption or accumulation of iron at their surfaces. Also, there was a significant positive correlation between the active iron concentration with SPAD numbers in the upper leaves (r = 0.88 **) and dry weight of soybean shoots (r = 0.87 **). Cultivars responses to Fe impregnated biochars showed that iron uptake and active iron concentration in the upper leaves of Williams variety were significantly less than those of Saman variety at both levels of Fe impregnated biochars (P ≤ 0.05), which indicates that cultivar Williams is more susceptible to the iron chlorosis. The results of this experiment and reports from other studies show that the application of impregnated biochars from nutrients besides increasing SOM, permeability and soil moisture, CEC and soil fertility level, also increases the acquisition and use efficiency of iron in the plant.
Conclusion: The results of this study showed that due to the strong adsorption of soil iron, non-impregnated biochar application in the level of 2.5% had no significant effect on the concentration and uptake of active iron and spad numbers of the plant. However, using Fe impregnated biochar and increasing their application in calcareous soils with iron chlorosis resulted in a significant increase of active soil iron content, concentration and uptake of active iron and SPAD numbers of the plant, and, conversely, a decrease of leaf chlorosis. Therefore, besides improving the physical, chemical and biological properties of the soil, the application of Fe impregnated biochar can also be a promising approach to eliminate iron chlorosis in sensitive plants, particularly soybeans in calcareous soils.
A. Jahandideh; M. Barani; E. Dordipour; R. Ghorbani Nasrabadi
Abstract
Introduction: One of the most important needs in the farm planning is the evaluation of different systems of plant nutrition. By supplying the correct way of plant nutrition, one can preserve the environment and increase the efficiency of agricultural inputs. Humic acid contains many nutrients that increase ...
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Introduction: One of the most important needs in the farm planning is the evaluation of different systems of plant nutrition. By supplying the correct way of plant nutrition, one can preserve the environment and increase the efficiency of agricultural inputs. Humic acid contains many nutrients that increase soil fertility, soil organic matter content, and access to macro- and micro-nutrients by preventing the formation of insoluble salts and chelating properties. Phosphorus and humic acid stimulate vegetative growth, improve reproductive growth, and increase the quantitative and qualitative yield of plants. In this regard, the positive effects depend on the amount and how they are applied. The present study was conducted with the aim of investigating the effects of different levels of humic acid and phosphorus fertilizer on phosphorus availability and photosynthetic pigments (a, b and carotenoids) in canola (cv. Hyola 50).
Methods and Materials: The soil used in this study was collected from 0-30 cm layer of a soil profile passed through a 2-mm sieve after air-drying. The soil chemical and physical properties were then determined. The pot experiment was conducted as factorial based on completely randomized design with three replications. Treatments include phosphorous fertilizer as super phosphate in three levels (0, 50 and 100 mg/kg) and humic acid in three levels (0. 0.5 and 1 gr/kg soil), phosphorous and humic acid application ways. Humic acid and phosphorous treatments were mixed in various forms including simultaneous mixing of humic acid and phosphorous fertilizer in the soil matrix, application of humic acid and phosphorous via irrigation water and coting of phosphorous fertilizer via solid humic acid before soil application. Then 10 canola seeds were planted in each pot at 2-cm depth which were declined to 4 plants in each pot after emerging and greening phases. At the end of the growth period (158 days), the plants were harvested. Determination of phosphorus concentrations of plant extracts by molybdenum vanadate or yellow method and chlorophyll content (a, b and ab) and carotenoids were measured precisely before harvesting using Barnes method. After harvesting the plants, the soil was immediately air-dried and passed through a 2mm sieve. Then, the amount of phosphorus was determined by sodium-DTPA and sodium bicarbonate. The statistical results of the data were analyzed using SAS software and LSD test (at 5% level) was used for comparing the mean values.
Results and Discussion: The interactions of humic acid and phosphorus and its application methods were significant for all measured traits at the 5% level. The results of the triple effects of humic acid levels and its application at the presence of phosphorus treatments showed that the highest chlorophyll (a, b and ab) and carotenoid content was obtained at 100 mg/kg phosphorus and 1 g/kg humic acid along with irrigation water. The highest concentration of plant shoot phosphorus with an average of 0.30% was observed in 1 g/kg humic acid with irrigation water at the level of 100 mg/kg phosphorus, although had no significant difference with 0.5 g/kg of humic acid with irrigation water. Maximum amount of P was extracted by Olsen method with the mean of 16.14 mg/kg and Soltanpour and Schwab method with the mean of 5.24 mg/kg obtained in 100 mg/kg phosphorus and 1 g/kg soil humic acid application. There was a significant correlation between the phosphorus extracted by Olsen method and Soltanpour and Schwab method (r = 0.95), which was significantly correlated with concentration of phosphorus (r = 0.84) and (r = 0.85) (P<0.05). There was also a significant correlation between fresh and dry above-ground biomass, types of chlorophyll (a, b and ab) and carotenoids with phosphorus extracted by Olsen and Soltanpour and Schwab methods at 5% significance level.
Conclusion: P adsorption capacity is a function of many factors. Application of phosphorous fertilizers in calcareous soils, due to the presence of calcium with high activity, results in the formation of calcium phosphates, which becomes insoluble, over time. Humic material in interaction with phosphorus in the soil can reduce phosphorus stabilization and increase plant available phosphorus. The results of this study showed that the use of phosphorus with humic acid, rather than the use of phosphorus alone, could increase the available phosphorus in the soil and also the phosphorus concentration within the plant.
Taleb Nazari; mojtaba barani; Esmaeil dordipour; Reza Ghorbani nasrabadi; Somayeh Sefidgar shahkolaie
Abstract
Introduction: Fe is the first identified micronutrient for crops and required in higher amount than other micronutrients. Fe plays important roles in enzyme metabolism, protein metabolism, chlorophyll construction, chloroplast evolution, photosynthesis, respiration and reduction-oxidation reaction as ...
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Introduction: Fe is the first identified micronutrient for crops and required in higher amount than other micronutrients. Fe plays important roles in enzyme metabolism, protein metabolism, chlorophyll construction, chloroplast evolution, photosynthesis, respiration and reduction-oxidation reaction as well as organic acids metabolism. Iron, as an essential micronutrient, has great contribution in important antioxidant enzymes activity and through which affects plant tolerance against environmental stresses. Plant enzymes including superoxide dismutase, catalase and glutathione peroxidase are among the most important enzymes scavenging the hydrogen peroxide have iron in their structure, so they affected by iron deficiency. In this study, the effect of soil, foliar and fertigation application of humic acid on iron availability, chlorophyll types and superoxide dismutase, catalase and glutathione peroxidase enzymes in canola (Hyola 308) were evaluated.
Results and Discussion: Results showed that highest total iron content in plant leaves was obtained in 0.4 percent foliar application and the lowest was belonged to control treatment. Highest iron content in plant stem and active iron was obtained in humic acid application through irrigation at 2000 mg L-1 by 85 and 44.86 mg kg-1, respectively, and lowest amounts were obtained in control by 54.62 and 20.40 mg kg-1. Also, greatest concentration of chlorophyll a, chlorophyll b and total chlorophyll were recorded under0.4 percent humic acid foliar application by 3.58, 1.79 and 5.37 and the lowest chlorophyll contents were associated to control. Highest activities for plant enzymes superoxide dismutase and glutathione peroxidase were obtained under0.1 percent foliar application of humic acid by 4.20 and 1.95 (Iu/gr. FW) and the highest activity for catalase enzyme by 4.46 Iu/gr FW in 1000 mg L-1 humic acid through was irrigation and the lowest enzyme activity obtained in control treatment. Findings showed that application of various levels of humic acid increased plant enzyme activity compared to control in all of three application method (soil, foliar and application through irrigation water). Increasing humic acid concentration decreased enzyme activities. Also, there was negative correlation between activity of plant enzymes and concentation of chlorophyll types and active iron.
Conclusions: Active iron and antioxidant enzymes represent iron status within cell cytoplasm. Based on the results of this study, active iron concentration and activity of antioxidant enzymes are appropriate indices for evaluating plant tolerance to iron deficiency compared to assessing total iron content in leaves.
zahra sharifi; Alireza Astaraei; A Fotovat; mojtaba baranimotlagh; Hojat Emami
Abstract
Introduction: Zinc is one of the essential micronutrients for plants, mining and industrial activities leading to pollution of heavy metals, including zinc metal contamination in soils. In addition to the total concentration, knowledge of the Zinc fractions is necessary to assess the mobility of zinc ...
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Introduction: Zinc is one of the essential micronutrients for plants, mining and industrial activities leading to pollution of heavy metals, including zinc metal contamination in soils. In addition to the total concentration, knowledge of the Zinc fractions is necessary to assess the mobility of zinc in the soils. One of the sequential extraction methods is Tessier method. sequential extraction with plant cultivation simultaneously, is the appropriate approach for assessing the mobility of toxic metals. Therefore this study was conducted to evaluate the chemical forms and determine their relationship to the physical and chemical properties of soils in some fields under cultivation in Khorasan Razavi province.
Materials and Methods: The experiment was conducted in a completely randomized design with factorial arrangement includes 4 levels of contamination (0, 500, 1000 and 1500 (mg/kg)) and 10 soil types from different regions of Khorasan Razavi province of 0-30 cm depth in the range of electrical conductivity 1 up to 15 ds/m, with three replications at Research greenhouses of Ferdowsi University of Mashhad. An example of mining waste was prepared as a source of pollution. The soil samples were kept at field capacity moisture for 6 months. Then air-dried soil samples were used for planting borage and determine the Zinc fractions. Then soil samples were air dried and used for planting borage and determining the Zinc fractions. Texture, cation exchange capacity, organic carbon, electrical conductivity, pH and Available phosphorus and potassium were measured in the saturation extract. DTPA-extractable Zinc was measured by atomic absorption spectrometry. Borage was planted in greenhouses in 3 kg pots with three replications. During flowering, the plants were harvested and dry digestion method was used to measure the concentration of Zinc. Chemical forms and Pseudo total concentration of zinc in the samples were determined using Tessier and digestion by HCl and HNO3 acids (3:1) methods respectively. The concentration of the extracts was measured by atomic absorption spectrometry. Statistical analysis was done using Minitab and Excel softwares.
Results and Discussion: Chemical Forms Average of zinc in soils was as follows:
Exchangeable < iron and manganese oxides < organic < carbonate < residual
Despite the low percentage of organic matter in these soils, in high levels of Zinc contamination a large amount of zinc was saved. Lack of organic Zinc, in addition to the low amount of organic matter soil is related to the dominance of iron oxides. In high levels of soil contamination, increased concentrations of zinc in all fractions, especially organic and carbonate which leads to an increase in the availability of zinc, is a serious threat to environment. The amount of exchangeable zinc was insignificant. Also the exchangeable, forms a small part of total amount of metal in the soils. The correlation between the chemical forms with each other and with the pseudo total, absorbed by plant roots and shoots and extracted with DTPA together, was significant. Absence of correlation between the exchangeable and iron and manganese oxides is probably indicative of the fact that the main supplier of soluble and exchangeable zinc normally after carbonates are iron and manganese oxides, that have little role in these soils. There is a significant positive correlation between different fractions of zinc with each other and this indicate a dynamic relationship between the zinc chemical forms in the soil. Correlation coefficients between plant available and chemical forms of zinc showed that plant available zinc derived from all fractions. A higher correlation coefficient between the plant available with carbonate and organic zinc was obtained, which indicates that carbonate and organic are the major suppliers for available plants zinc.
Conclusion: In this study, the residual, carbonate and organic fractions are dominant form of zinc in soils, respectively. With increasing level of contamination, percentage of residual zinc decreased and percentage of other fractions increased, particularly organic and carbonate. Increasing the availability of zinc, is a threat to the environment. There is a high correlation coefficient between different fractions of Zinc with each other and with the pseudo total, amount of plant and available plant zinc showed that there is a dynamic relationship in the soil systems. There is a higher correlation coefficient between the available zinc and carbonate and organic fractions of soils, which indicate available plant zinc, are mainly derived from carbonate and organic fractions.
Somayeh Sefidgar; Mojtaba Barani Motlagh; farhad khormali; Esmael Dordipour
Abstract
Introduction: Soil pollution with heavy metals have become a global concern because of its damaging effects on the environment, including human health, toxicity in plants and long-term effects on soil fertility. Heavy metals stress in plants is characterized by decrease in photosynthesis, nutrient uptake, ...
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Introduction: Soil pollution with heavy metals have become a global concern because of its damaging effects on the environment, including human health, toxicity in plants and long-term effects on soil fertility. Heavy metals stress in plants is characterized by decrease in photosynthesis, nutrient uptake, damaging of roots and finally plant death. Lead (Pb) is found to be the most dangerous heavy metal, responsible for reduced soil fertility and elevated environmental pollution. Lead toxicity causes the inhibition of seed germination and exerts adverse effects on growth and metabolic processes of plants, which retards plant and crop production. The overproduction of reactive oxygen species (ROS) is the best indicator for secondary stress, which results in a number of toxic effects on biochemical processes in many plant cells. The overproduction of ROS due to Pb stress brings about changes in cellular membrane permeability, which in turn damages organelles such as nuclei, mitochondria, and chloroplasts in plant cells which decreased plant growth and yield. Chemical stabilization is an in situ remediation method that uses inexpensive amendments to reduce contaminant availability in polluted soil. The aim of this study was to investigate the immobilization of lead in a calcareous contaminated soil using two types of biochar as organic and Pumice, Leca, Zeolite and Bentonite as inorganic amendments.
Materials and Methods: In order to investigate the effect of organic amendments (biochar 640°C, and biochar 420°C) and inorganic amendments (Pumice, Leca, Zeolite and Bentonite) on Pb stabilization in a contaminated soil (1500 mg/kg), a greenhouse experiment using maize plant was carried out. This experiment was conducted in a completely randomized design consisting of 6 types of amendments (Pumice, Leca, Zeolite, Bentonite, Biochar 420°C, and Biochar 640°C) and at 1% and 5% levels of each amendment (12 amendments plus 1 control). The experimental treatments were incubated for 3 months. At the end of incubation time, the potential bioavailability of Pb in non-amended and amended soils was assessed by chemical extractions, as: extraction with DTPA, with ammonium acetate and with ethylenediaminetetraacetic acid (EDTA). After the end of incubation time, the pots were transferred to a greenhouse and in each pot five maize seeds were planted and then reduced to three seedlings in each pot after germination. After 3 months, all the plants were harvested. The Pb concentration in each plant, its biomass, its chlorophyll and its antioxidant enzyme activities levels were analyzed. All statistical analyses were performed using SAS software. Means of different treatments were compared using LSD (P ≤0.05) test.
Results and Discussion: The results indicated that the addition of amendments to soils reduced the concentration of Pb extracted with DTPA and EDTA. The 5% biochar 640 had the greatest reduction effect on DTPA-extractable Pb. The smallest concentration of Pb in the leaves and root of maize plant was observed in treated soil with organic amendments (biochar 640°C, and biochar 420°C) and treated with 5% zeolite, respectively. The highest increase in plant growth parameters like SPAD value, leaf area, plant height, number of leaves per plant, dry biomass yield and dry matter of roots were observed in organic amendments compared to the control. The application of 5% amendments in soil caused a significant increase in plant height and number of leaves as compared to control. The increase in growth and biomass of zea mays L. under various amendments might be due to decreased bioavailable Pb concentrations in soil amended which may be attributed to reduced Pb toxicity through improvement of soil fertility. Also, the application of amendments resulted in a significant increase in antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), peroxidase (PX), and ascorbate peroxidase (ASP) in maize plants compared to the control. The increase of leaves enzyme activities with addition amendments may be due to a lower Pb accumulation in leaves because excess Pb generates free radicals and reactive oxygen species (ROS) those causes oxidative stress in plants.
Conclusions: The results indicated that the application of amendments were successful in lowering the potential bioavailability of Pb in the soils. The 5% biochar 640 treatment had the greatest reduction effect on extractable Pb. The application of amendments decreased the uptake and accumulation of Pb in maize plants, via the reduction of DTPA- extractable Pb. The amendments also significantly increased leaves antioxidant enzyme activities and photosynthetic pigments compared to the control.
S. Tajari; mojtaba barani; F. Khormali; F. Kiani
Abstract
Introduction: P in soils exists in many complex chemical forms, which differ markedly in their behavior, mobility and resistance to bioavailability in the soils. The total P content of a soil provides little information regarding the behavior of P in the environment. The various forms of P present to ...
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Introduction: P in soils exists in many complex chemical forms, which differ markedly in their behavior, mobility and resistance to bioavailability in the soils. The total P content of a soil provides little information regarding the behavior of P in the environment. The various forms of P present to a large degree, determine the fate and transport of P in soils. Fractionation schemes using different chemical sequential extractions have been used in order to describe the many different forms in which P can be found in the soil. The reason for fractionating and studying P forms in the soil is usually to allow a more precise description of the potentials for P release from the soil. The forms and dynamics of soil P can be greatly affected by agricultural management practices. Since inorganic P is the preferred source for plant uptake, knowledge of the inorganic form within soils is fundamental to understanding bioavailability of P and sustainability of agricultural practice. The aim of this study was to investigate the effect of land use change on the form and distribution of inorganic P using a sequential extraction procedure.
Materials and Methods: In order to study the impact of land-use change from forestland to cultivated land, composite samples in four replicates from the upper 10 cm of the different land use systems (natural forest, pasture, bower olive, farmland) were collected. We collected five subsamples from each land use in a radial sampling scheme. The five subsamples were then bulked into one sample. The spacing between the subsamples on the radii ranged from 5 to 10 m. The soil samples were transferred to polyethylene bags and transported to the laboratory where they were slightly crushed, passed through a 2 mm sieve prior to fractionation and chemical analysis. Soil texture, cation exchange capacity, organic carbon (OC), electrical conductivity, pH and calcium carbonate equivalent (CCE) were measured with standard methods. Total P and total inorganic P (Pi) contents were measured by the ignition method, for which P in the ignited (550 °C) and unignited soil samples were extracted by 0.5 M H2SO4. A modified version of the sequential extraction of Olsen and Sommers (1982) was used to fractionate inorganic P. Phosphorus was measured in the extracted supernatants by the molybdate–ascorbic acid method.
Results and Discussion: The results showed that clear-cutting of the indigenous forests and their conversion into agricultural fields significantly decreased total P and total organic P levels. Land-use changes from natural forest to farmland decreased the total P by 23% (from 644 to 495 mg per kg). Clearing and subsequent cultivation of the native woodland resulted in a marked depletion of total organic P. In addition, the land-use conversion from the natural forestland to an agroecosystem (cultivated land) led to increases in total inorganic P and inorganic P forms levels (labile P, P non-occluded, occluded in oxides of iron and aluminum, soluble calcium phosphate and sparingly soluble calcium phosphate). Labile inorganic P (NaHCO-Pi) showed the greatest changes, such as labile inorganic P in the amount of change from 1.75 in the forest land to 13.01 mg per kg of cultivated land, which represent an increase of approximately 8-fold compared to control (natural forest). The results also revealed that the refractory inorganic P fractions (HCl-Pi) were the major inorganic P pool, comprising 50-70% of the total inorganic P pool, indicating CaCO3 control over phosphorus availability in the studied soils. This study indicated that forestland degradation and cultivation caused chemical changes of P dynamics.
Conclusion: Large-scale conversion of indigenous forests to cultivated land, driven by long-term agricultural development in the Toshan region, has greatly impacted the forms and content of P in the soils. Generally, the conversion of natural ecosystem to agroecosystems, decreased the proportion of organic P (Po) in the top-soils at depth of 0 to 10 cm. The depletion in organic P from the cropped fields could be attributed to the enhanced mineralization of soil organic P caused by cultivation and removal of P in the crops. However, the conversion of natural forest to farmland led to increases in inorganic P (Pi). About 50% to 70% of the TP was bound to CaCO3, and thus this solid phase is critical to P fate in the soils and ecosystem of the Toshan Region, Golestan province
